Rabbit Recombinant Monoclonal antibody. Suitable for IHC-P, Dot, WB and reacts with Rat, Mouse, Human, Synthetic peptide samples. Cited in 59 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IHC-P | Dot | WB | |
---|---|---|---|
Human | Tested | Expected | Tested |
Mouse | Tested | Expected | Tested |
Rat | Tested | Expected | Tested |
Synthetic peptide | Not recommended | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/5000 | Notes Use of HRP-conjugated or polymerized HRP secondary antibodies recommended, stronger signals have been found using the polymerized HRP secondary. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/5000 | Notes Use of HRP-conjugated or polymerized HRP secondary antibodies recommended, stronger signals have been found using the polymerized HRP secondary. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1/5000 | Notes Use of HRP-conjugated or polymerized HRP secondary antibodies recommended, stronger signals have been found using the polymerized HRP secondary. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Select an associated product type
Rabbit Recombinant Monoclonal antibody. Suitable for IHC-P, Dot, WB and reacts with Rat, Mouse, Human, Synthetic peptide samples. Cited in 59 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EP2606Y
Affinity purification Protein A
Detects PKA alpha/beta/gamma (catalytic subunit) phosphorylated on threonine 197. Moreover, the antibody will cross-react with PRKX/Y (pT203).
Blue Ice
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
The protein kinase A (PKA) alpha/beta/gamma also known as the PKA catalytic subunit plays a significant role in the regulation of cellular functions through phosphorylation. It has alternate names including phospho PKA and PKA C alpha with an approximate molecular weight of 40 kDa. The PKA catalytic subunit is expressed in various tissues throughout the body. This distribution highlights its broad physiological impact given PKA's involvement in numerous cellular processes.
PKA alpha/beta/gamma acts as an important regulatory molecule in signal transduction pathways. It functions by phosphorylating serine and threonine residues on target proteins which alters the activity protein interactions or localization of these substrates. PKA functions as part of the cAMP-dependent PKA holoenzyme complex which includes two regulatory (R) and two catalytic (C) subunits. Activation requires the binding of cAMP to the R subunits causing dissociation and releasing the active C subunits to exert their effects.
PKA alpha/beta/gamma integrates into the cAMP signaling pathway which modulates numerous physiological processes such as metabolism gene expression and cell growth. In the cAMP pathway PKA interacts closely with proteins like adenylyl cyclase and phosphodiesterases which respectively increase and decrease cAMP levels affecting PKA activation state and subsequent cellular responses. PKA also plays roles within the MAPK/ERK pathway where it intersects with other kinases to influence cell cycle progression and differentiation.
PKA alpha/beta/gamma has connections to cancer and cardiovascular diseases. Mutations or dysregulation of PKA activity can lead to abnormal cell proliferation and survival contributing to tumorigenesis. The catalytic subunit is also linked to cardiovascular disorders through its impact on heart rate and contractility. Interaction with proteins such as AKAPs (A-kinase anchoring proteins) can localize PKA activity affecting local signaling cascades that influence disease pathology.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991) at 1/1000 dilution
Lane 1: Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 100nM Calyculin A for 30 minutes, whole cell lysate at 15 µg
Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with Lambda Phosphatase for 1 hour at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
This data was developed using ab75991, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, then the membrane treated with Lambda Phosphatase for 1 hour at 15 µg
Lane 3: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg
Lane 4: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, then the membrane treated with Lambda Phosphatase for 1 hour at 15 µg
Lane 5: C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg
Lane 6: C6 (Rat glial tumor glial cell) whole cell lysate, then the membrane treated with Lambda Phosphatase for 1 hour at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
This data was developed using ab75991, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling PKA alpha/beta/gamma (catalytic subunit) with Purified ab75991 at 1/5000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling PKA alpha/beta/gamma (catalytic subunit) with Purified ab75991 at 1/5000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling PKA alpha/beta/gamma (catalytic subunit) with Purified ab75991 at 1/5000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991) at 1/1000 dilution
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) 10% FBS all the time. Whole cell lysates at 15 µg
Lane 2: Jurkat (Human T cell leukemia T lymphocyte) serum starve for 24h then 20% FBS 20h. Whole cell lysates at 15 µg
Lane 3: Jurkat (Human T cell leukemia T lymphocyte) serum starve for 24h then 20% FBS 20h.Whole cell lysates. Then the membrane was incubated with phosphatase. at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 1s
Dot blot analysis of various peptides labelling PKA gamma (catalytic subunit) (phospho T197) with unpurified ab75991 at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/100000).
Lane 1 - PKA alpha/beta/gamma (catalytic subunit) (pT197) phospho peptide.
Lane 2 - PKA alpha/beta/gamma (catalytic subunit) non-phospho peptide.
Lane 3 - PRKX/Y (pT203) phospho peptide.
Lane 4 - PRKX/Y non-phospho peptide.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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