AB187515

Anti-PKA beta (catalytic subunit) antibody

Name: Anti-PKA beta (catalytic subunit) antibody (ab187515) | Abcam
Brand: Abcam Limited
SKU: AB187515
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(11 Publications)

Rabbit Polyclonal PKA beta (catalytic subunit) antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRKACB.

View Alternative Names

cAMP-dependent protein kinase catalytic subunit beta, PKA C-beta, PRKACB

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-PKA beta (catalytic subunit) antibody (AB187515)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PKA beta (catalytic subunit) antibody (AB187515)

Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab187515. Blue DAPI for nuclear staining.

Lab

Western blot - Anti-PKA beta (catalytic subunit) antibody (AB187515)

False colour image of Western blot : Anti-PKA beta (catalytic subunit) antibody staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab187515 was shown to bind specifically to PKA beta (catalytic subunit). A band was observed at 41 kDa in wild-type HeLa cell lysates with no signal observed at this size in PRKACB knockout cell line ab264673 (knockout cell lysate ab258603). To generate this image, wild-type and PRKACB knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tweenï ¿½ 20 (TBS-T) before incubation with primary antibodies overnight at 4 ï ¿½C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-PKA beta (catalytic subunit) antibody (ab187515) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PRKACB (PKA beta catalytic subunit) knockout HeLa cell lysate (<a href='/en-us/products/cell-lysates/human-prkacb-pka-beta-catalytic-subunit-knockout-hela-cell-lysate-ab258603'>ab258603</a>) at 20 µg

Lane 3:

Wild-type HAP1 cell lysate at 20 µg

Lane 4:

PRKACB knockout HAP1 cell lysate at 20 µg

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Supplier Data

Western blot - Anti-PKA beta (catalytic subunit) antibody (AB187515)

All lanes:

Western blot - Anti-PKA beta (catalytic subunit) antibody (ab187515) at 1/500 dilution

All lanes:

SW480 cell extract

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PRKACB.

P22694

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PKA beta (catalytic subunit) also known as PRKACB or Protein Kinase A catalytic subunit beta plays a pivotal role in cellular signaling mechanisms. Mechanically it catalyzes the transfer of a phosphate group from ATP to a serine or threonine residue in target proteins impacting various cellular processes. This catalytic subunit has a molecular weight of approximately 40 kDa. It expresses widely in many tissues including the brain heart and skeletal muscle where it contributes to regulating metabolic and transcriptional activities.

Biological function summary

The PKA beta catalytic subunit functions in a larger context as part of the Protein Kinase A (PKA) complex which is a central player in the cyclic AMP (cAMP) signaling pathway. This complex acts as an effector of cAMP translating extracellular signals into intracellular actions by phosphorylating a wide range of target proteins. As a part of this complex it modulates activities related to glucose metabolism cell proliferation and gene expression reflecting its involvement in many cellular processes.

Pathways

PKA beta is integrally involved in the cAMP signaling and insulin signaling pathways. In the cAMP pathway it activates by binding to cAMP leading to dissociation from regulatory subunits and subsequent activation of substrate proteins. In the insulin signaling pathway it interacts with proteins like PI3K and Akt playing an important role in regulating glucose homeostasis and lipid metabolism. These pathways illustrate the PKA beta's importance in maintaining cellular and systemic metabolic balance.

The dysfunction of PKA beta is associated with conditions such as cardiac hypertrophy and certain types of cancer. Elevations or disruptions in PKA beta activity can lead to alterations in cell growth and metabolic regulation. PKA beta's relationship with proteins like CREB and Bcl-2 through these disorders has been observed where aberrant phosphorylation mediated by PKA can drive pathological cell survival proliferation and growth linking malfunction directly to disease progression.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs (PubMed : 12420224, PubMed : 21423175, PubMed : 31112131). PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux (PubMed : 12420224, PubMed : 21423175). Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis (PubMed : 12420224, PubMed : 21423175). Phosphorylates GPKOW which regulates its ability to bind RNA (PubMed : 21880142). Acts as a negative regulator of mTORC1 by mediating phosphorylation of RPTOR (PubMed : 31112131).

See full target information PRKACB

style

left-column

Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101593 PubMed38843842

2024

Ketogenic diet administration later in life improves memory by modifying the synaptic cortical proteome via the PKA signaling pathway in aging mice.

Applications

Unspecified application

Species

Unspecified reactive species

Diego Acuña-Catalán,Samah Shah,Cameron Wehrfritz,Mitsunori Nomura,Alejandro Acevedo,Cristina Olmos,Gabriel Quiroz,Hernán Huerta,Joanna Bons,Estibaliz Ampuero,Ursula Wyneken,Magdalena Sanhueza,Felipe Arancibia,Darwin Contreras,Julio César Cárdenas,Bernardo Morales,Birgit Schilling,John C Newman,Christian González-Billault

Open life sciences 19:20220878 PubMed38840893

2024

Role of Embinin in the reabsorption of nucleus pulposus in lumbar disc herniation: Promotion of nucleus pulposus neovascularization and apoptosis of nucleus pulposus cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Meng,Wei Liu,Haifeng Liu,Chengwei Yu

Biology of reproduction 110:154-168 PubMed37815939

2023

Phoenixin-14 as a novel direct regulator of porcine luteal cell functions†.

Applications

Unspecified application

Species

Unspecified reactive species

Ewa Mlyczyńska,Patrycja Kurowska,Dominika Wachowska,Małgorzata Grzesiak,Joelle Dupont,Agnieszka Rak

The Journal of endocrinology 247:283-294 PubMed33108345

2020

The role of vaspin in porcine corpus luteum.

Applications

Unspecified application

Species

Unspecified reactive species

Patrycja Kurowska,Ewa Mlyczyńska,Monika Dawid,Małgorzata Grzesiak,Joelle Dupont,Agnieszka Rak

The Journal of biological chemistry 295:9433-9444 PubMed32409581

2020

AKT Ser/Thr kinase increases V-ATPase-dependent lysosomal acidification in response to amino acid starvation in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Michael P Collins,Laura A Stransky,Michael Forgac

Journal of inflammation (London, England) 17:18 PubMed32377171

2020

Inhibition of carrageenan-induced dental inflammatory responses owing to decreased TRPV1 activity by Dexmedetomidine.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Lv,Guanhua Zhu,Maohua Xu,Xingping Gao,Qingfeng Xiao

Biology of reproduction 102:1290-1305 PubMed32149334

2020

Role of vaspin in porcine ovary: effect on signaling pathways and steroid synthesis via GRP78 receptor and protein kinase A†.

Applications

Unspecified application

Species

Unspecified reactive species

Patrycja Kurowska,Ewa Mlyczyńska,Monika Dawid,Joelle Dupont,Agnieszka Rak

Aging cell 19:e13055 PubMed31668016

2019

Tau inhibits PKA by nuclear proteasome-dependent PKAR2α elevation with suppressed CREB/GluA1 phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jinwang Ye,Yaling Yin,Huanhuan Liu,Lin Fang,Xiaoqing Tao,Linyu Wei,Yue Zuo,Ying Yin,Dan Ke,Jian-Zhi Wang

Artificial cells, nanomedicine, and biotechnology 47:2048-2057 PubMed31126199

2019

K-Ras-PI3K/AKT pathway regulates H1.4 through PKA to promote the occurrence and development of osteosarcoma cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jingzhe Zhang,Wanguo Liu,Hang Dong,Wenjun Wang

Physiological reports 7:e13972 PubMed30806037

2019

Exercise training reduces ventricular arrhythmias through restoring calcium handling and sympathetic tone in myocardial infarction mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rujie Qin,Nobuyuki Murakoshi,DongZhu Xu,Kazuko Tajiri,Duo Feng,Endin N Stujanna,Saori Yonebayashi,Yoshimi Nakagawa,Hitoshi Shimano,Akihiko Nogami,Akira Koike,Kazutaka Aonuma,Masaki Ieda

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PKA beta (catalytic subunit) antibody

Immunocytochemistry/ Immunofluorescence - Anti-PKA beta (catalytic subunit) antibody (AB187515)

Western blot - Anti-PKA beta (catalytic subunit) antibody (AB187515)

Western blot - Anti-PKA beta (catalytic subunit) antibody (AB187515)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise