Anti-PKA beta (catalytic subunit) (phospho S338) antibody

• WB

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Western blot - Anti-PKA beta (catalytic subunit) (phospho S338) antibody (AB5816)

Peptide Competition : Lysates prepared from 3T3-L1 cells were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either treated with lambda phosphatase (1) or left untreated (2-5), blocked with a 5% BSA-TBSTbuffer for two hours at room temperature, and incubated with ab5816 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with : no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP-conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the peptide corresponding to PKA cat beta [pS338] blocks the antibody signal, thereby verifying the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-

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Western blot - Anti-PKA beta (catalytic subunit) (phospho S338) antibody (ab5816)

Predicted band size: 41 kDa

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