AB236855

Anti-PKA R2/PKR2 antibody

Name: Anti-PKA R2/PKR2 antibody (ab236855) | Abcam
Brand: Abcam Limited
SKU: AB236855
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal PKA R2/PKR2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PRKAR2A aa 1-250.

View Alternative Names

PKR2, PRKAR2, PRKAR2A, cAMP-dependent protein kinase type II-alpha regulatory subunit

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 antibody (AB236855)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 antibody (AB236855)

Paraffin-embedded human glioma tissue stained for PKA R2/PKR2 using ab236855 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-PKA R2/PKR2 antibody (AB236855)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PKA R2/PKR2 antibody (AB236855)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for PKA R2/PKR2 (green) using ab236855 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor^® 488-congugated Goat Anti-Rabbit IgG (H+L).

Supplier Data

Western blot - Anti-PKA R2/PKR2 antibody (AB236855)

All lanes:

Western blot - Anti-PKA R2/PKR2 antibody (ab236855) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 3:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 45 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 antibody (AB236855)

Paraffin-embedded human lymph node tissue stained for PKA R2/PKR2 using ab236855 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PRKAR2A aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P13861

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein Kinase A R2 (PKR2) also known as PRKAR2A is a regulatory subunit of Protein Kinase A (PKA). PKA is part of a larger complex known as the cAMP-dependent protein kinase which plays an important role in cellular signaling. PKA consists of regulatory and catalytic subunits with PKR2 serving as one of its regulatory forms. PKR2 possesses a molecular mass of approximately 47 kDa and is available in various human tissues with notable expression in the brain and cardiovascular system.

Biological function summary

PKR2 regulates the activity of PKA by binding to the catalytic subunit in the absence of cAMP therefore inhibiting its activity. When cAMP is present it binds to the regulatory subunit PKR2 leading to the release and activation of the catalytic subunit of PKA. PKR2 is an integral part of the PKA holoenzyme complex which plays a critical role in mediating cellular responses to hormonal stimulation.

Pathways

PKR2 is heavily involved in the cAMP-dependent signaling pathway. This pathway is essential for translating extracellular signals into cellular actions. It interacts with various effector proteins including adenylate cyclases and phosphodiesterases to modulate responses such as gene transcription metabolism and memory formation. PKR2-related activity influences pathways shared with proteins like the G-protein subunits which facilitate the effects of neurotransmitters.

PKR2 irregularities can impact conditions like heart failure and neurodegenerative diseases. Disrupted PKA pathway signaling involving PKR2 can contribute to cardiac dysfunction by affecting heart muscle contractility and hypertrophy. In neurodegenerative diseases the improper functioning of PKR2 can exacerbate disruptions in neural signaling. A connection exists between PKR2 and proteins such as CREB (cAMP response element-binding protein) which links PKR2's role to memory deficits observed in neurodegeneration.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

See full target information PRKAR2A

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1171308 PubMed37325657

2023

Involvement of CD40-CD40L and ICOS-ICOSL in the development of chronic rhinosinusitis by targeting eosinophils.

Applications

Unspecified application

Species

Unspecified reactive species

Aina Zhou,Chenxi Shi,Yuhui Fan,Yushuang Zheng,Jue Wang,Zhichen Liu,Huanxia Xie,Jisheng Liu,Qingqing Jiao

Experimental dermatology 31:1253-1263 PubMed35514241

2022

Silencing of Rab23 by siRNA inhibits ultraviolet B-induced melanogenesis via downregulation of PKA/CREB/MITF.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanjin Wei,Min Huang,Yi Yang,Yali Liu,Sumin Chi,Chengxin Li

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PKA R2/PKR2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 antibody (AB236855)

Immunocytochemistry/ Immunofluorescence - Anti-PKA R2/PKR2 antibody (AB236855)

Western blot - Anti-PKA R2/PKR2 antibody (AB236855)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 antibody (AB236855)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise