AB38949

Anti-PKA R2/PKR2 antibody

Name: Anti-PKA R2/PKR2 antibody (ab38949) | Abcam
Brand: Abcam Limited
SKU: AB38949
Price: 535 USD
Availability: InStock
Rating: 4 (7 reviews)

(7 Reviews)

(19 Publications)

Rabbit Polyclonal PKA R2/PKR2 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 19 publications.

View Alternative Names

PKR2, PRKAR2, PRKAR2A, cAMP-dependent protein kinase type II-alpha regulatory subunit

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-PKA R2/PKR2 antibody (AB38949)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PKA R2/PKR2 antibody (AB38949)

ICC/IF image of ab38949 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38949, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PKA R2/PKR2 antibody (AB38949)

ICC/IF image of ab38949 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38949, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab38949 binds to PKARIIa, but does not cross react with the other PKA regulatory subunits (PKARIa, PKARIb, PKARIIb) and can recognize native or reduced PKARIIa.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Store diluted antibody at 4°C with 0.05% Sodium Azide as preservative.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab38949 has been peptide-affinity purified.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein Kinase A R2 (PKR2) also known as PRKAR2A is a regulatory subunit of Protein Kinase A (PKA). PKA is part of a larger complex known as the cAMP-dependent protein kinase which plays an important role in cellular signaling. PKA consists of regulatory and catalytic subunits with PKR2 serving as one of its regulatory forms. PKR2 possesses a molecular mass of approximately 47 kDa and is available in various human tissues with notable expression in the brain and cardiovascular system.

Biological function summary

PKR2 regulates the activity of PKA by binding to the catalytic subunit in the absence of cAMP therefore inhibiting its activity. When cAMP is present it binds to the regulatory subunit PKR2 leading to the release and activation of the catalytic subunit of PKA. PKR2 is an integral part of the PKA holoenzyme complex which plays a critical role in mediating cellular responses to hormonal stimulation.

Pathways

PKR2 is heavily involved in the cAMP-dependent signaling pathway. This pathway is essential for translating extracellular signals into cellular actions. It interacts with various effector proteins including adenylate cyclases and phosphodiesterases to modulate responses such as gene transcription metabolism and memory formation. PKR2-related activity influences pathways shared with proteins like the G-protein subunits which facilitate the effects of neurotransmitters.

PKR2 irregularities can impact conditions like heart failure and neurodegenerative diseases. Disrupted PKA pathway signaling involving PKR2 can contribute to cardiac dysfunction by affecting heart muscle contractility and hypertrophy. In neurodegenerative diseases the improper functioning of PKR2 can exacerbate disruptions in neural signaling. A connection exists between PKR2 and proteins such as CREB (cAMP response element-binding protein) which links PKR2's role to memory deficits observed in neurodegeneration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

See full target information PRKAR2A

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 30:e14362 PubMed37469037

2023

MPP2 interacts with SK2 to rescue the excitability of glutamatergic neurons in the BLA and facilitate the extinction of conditioned fear in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohan Peng,Panpan Chen,Yang Zhang,Ke Wu,Ningning Ji,Jinghua Gao,Hui Wang,Yong-Mei Zhang,Tie Xu,Rong Hua

Journal of molecular neuroscience : MN 72:1797-1808 PubMed35727524

2022

Propofol Protects Against Erastin-Induced Ferroptosis in HT-22 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Xuan,Xinyi Lu,Zeyong Yang,Jun Li,Weilin Jin,Yuanhai Li

eLife 11: PubMed35199643

2022

Complex effects of kinase localization revealed by compartment-specific regulation of protein kinase A activity.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca LaCroix,Benjamin Lin,Tae-Yun Kang,Andre Levchenko

BioMed research international 2020:7169348 PubMed33102590

2020

Protective Effects of Adiponectin against Cobalt Chloride-Induced Apoptosis of Smooth Muscle Cells via cAMP/PKA Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjie Xiao,Yingying Zhang,Wei Zhang,Liang Zhang,Li Li,Junqiang Si,Xinzhi Li,Ketao Ma

Evidence-based complementary and alternative medic 2020:9837384 PubMed32774435

2020

Maren Pills Improve Constipation via Regulating AQP3 and NF-B Signaling Pathway in Slow Transit Constipation In Vitro and In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhan,Xuegui Tang,Hong Xu,Shiyu Tang

Experimental and therapeutic medicine 17:748-758 PubMed30651859

2019

Effect of different charges of modified electroconvulsive seizure on the cognitive behavior in stressed rats: Effects of GluR1 phosphorylation and CaMKIIα activity.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Zhang,Guihua Huang,Xianlin Zhu

Cellular physiology and biochemistry : internation 51:1600-1615 PubMed30497067

2018

Inhibition of microRNA-200a Upregulates the Expression of Striatal Dopamine Receptor D2 to Repress Apoptosis of Striatum via the cAMP/PKA Signaling Pathway in Rats with Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Mei Wu,Shan Wang,Xin Wen,Xin-Rui Han,Yong-Jian Wang,Min Shen,Shao-Hua Fan,Juan Zhuang,Zi-Feng Zhang,Qun Shan,Meng-Qiu Li,Bin Hu,Chun-Hui Sun,Jun Lu,Yuan-Lin Zheng

Oncology reports 39:2171-2177 PubMed29532894

2018

Subcellular localization of aquaporin 3 in prostate cancer is regulated by RalA.

Applications

Unspecified application

Species

Unspecified reactive species

Qiwei Chen,Liang Zhu,Huafeng Zong,Xishuang Song,Lina Wang,Xuejian Wang,Deyong Yang,Jianbo Wang

Molecular metabolism 6:125-137 PubMed28123943

2017

Systemic insulin sensitivity is regulated by GPS2 inhibition of AKT ubiquitination and activation in adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Carly T Cederquist,Claudia Lentucci,Camila Martinez-Calejman,Vanessa Hayashi,Joseph Orofino,David Guertin,Susan K Fried,Mi-Jeong Lee,M Dafne Cardamone,Valentina Perissi

eLife 6: PubMed28079521

2017

Isoform-specific subcellular localization and function of protein kinase A identified by mosaic imaging of mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Ronit Ilouz,Varda Lev-Ram,Eric A Bushong,Travis L Stiles,Dinorah Friedmann-Morvinski,Christopher Douglas,Jeffrey L Goldberg,Mark H Ellisman,Susan S Taylor

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PKA R2/PKR2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PKA R2/PKR2 antibody (AB38949)

Immunocytochemistry/ Immunofluorescence - Anti-PKA R2/PKR2 antibody (AB38949)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

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