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AB326622

Anti-PKA R2/PKR2 (phospho S99) antibody [SC54-04]

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Rabbit Recombinant Monoclonal PKA R2/PKR2 phospho S99 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples.

View Alternative Names

PKR2, PRKAR2, PRKAR2A, cAMP-dependent protein kinase type II-alpha regulatory subunit

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 (phospho S99) antibody [SC54-04] (AB326622)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 (phospho S99) antibody [SC54-04] (AB326622)

Formalin-fixed, paraffin-embedded human spleen tissue stained for PKA R2/PKR2 (phospho S99) using antibody at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-PKA R2/PKR2 (phospho S99) antibody [SC54-04] (AB326622)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PKA R2/PKR2 (phospho S99) antibody [SC54-04] (AB326622)

ICC/IF staining of PKA R2/PKR2 (phospho S99) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with antibody at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 (phospho S99) antibody [SC54-04] (AB326622)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 (phospho S99) antibody [SC54-04] (AB326622)

Formalin-fixed, paraffin-embedded mouse testis tissue stained for PKA R2/PKR2 (phospho S99) using antibody at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 (phospho S99) antibody [SC54-04] (AB326622)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 (phospho S99) antibody [SC54-04] (AB326622)

Formalin-fixed, paraffin-embedded mouse heart tissue stained for PKA R2/PKR2 (phospho S99) using antibody at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-PKA R2/PKR2 (phospho S99) antibody [SC54-04] (AB326622)
  • WB

Supplier Data

Western blot - Anti-PKA R2/PKR2 (phospho S99) antibody [SC54-04] (AB326622)

All lanes : Primary antibody at 1/1000 dilution
Lane 1 : Mouse Brain lysates
Lane 2 : Rat Brain lysates
Lane 3 : K562 treated with Lambda Protein Phosphatase for 30min whole cell
Lane 4 : K562 whole cell lysates
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size : 46 kDa
Observed band size : 51 kDa

Exposure time : 7 seconds

All lanes:

Western blot - Anti-PKA R2/PKR2 (phospho S99) antibody [SC54-04] (ab326622) at 1/1000 dilution

Lane 1:

Mouse Brain lysates

Lane 2:

Rat Brain lysates

Lane 3:

K562 treated with Lambda Protein Phosphatase for 30min whole cell

Lane 4:

K562 whole cell lysates

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 46 kDa

Observed band size: 51 kDa

false

Exposure time: 7s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SC54-04

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
See full target information PRKAR2A pS99
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com