Anti-PKC alpha antibody [133]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [133] (AB11723)

Immunohistochemical analysis of Paraffin-embedded human retina tissue labelling PKC alpha with ab11723 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human retina is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) ab214879.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [133] (AB11723)

Immunohistochemical analysis of paraffin-embedded rat retina tissue labelling PKC alpha with ab11723 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat retina is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) ab214879.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [133] (AB11723)

Immunohistochemical analysis of paraffin-embedded mouse retina tissue labelling PKC alpha with ab11723 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse retina is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) ab214879.

• WB

Lab

Western blot - Anti-PKC alpha antibody [133] (AB11723)

This image was generated from the hybridoma version of the product.

Lanes 1, 5 and 9 : Wild-type HAP1 cell lysate (20 μg)
Lanes 2, 6 and 10 : PKC alpha knockout HAP1 cell lysate (20 μg)
Lanes 3, 7 and 11 : K562 cell lysate (20 μg)
Lanes 4, 8 and 12 : HEK293 cell lysate (20 μg)
Lanes 1, 2, 3 and 4 : Green signal from target – ab11723 observed at 77 kDa
Lanes 5, 6, 7 and 8 : Red signal from loading control – ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12 : Merged (red and green) signal

ab11723 was shown to specifically react with PKC alpha in wild-type HAP1 cells. No band was observed when PKC alpha knockout samples were examined. Wild-type and PKC alpha knockout samples were subjected to SDS-PAGE. ab11723 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PKC alpha antibody [133] (ab11723)

Predicted band size: 76 kDa

false

• WB

Lab

Western blot - Anti-PKC alpha antibody [133] (AB11723)

Exposure time

Lane 1 : 136 seconds
Lane 2 and 3 : 15 seconds

Fresh lysates were used in lane 2 and 3.

All lanes:

Western blot - Anti-PKC alpha antibody [133] (ab11723) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor glial cell), whole cell lysate 20 μg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate 20 μg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 20 μg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 76 kDa

false

• WB

Lab

Western blot - Anti-PKC alpha antibody [133] (AB11723)

5% NFDM/TBST

All lanes:

Western blot - Anti-PKC alpha antibody [133] (ab11723) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 76 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-PKC alpha antibody [133] (AB11723)

This image was generated from the hybridoma version of the product.

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : PKC alpha knockout HAP1 cell lysate (20 μg)
Lane 3 : K562 cell lysate (20 μg)
Lane 4 : HEK293 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab11723 observed at 77 kDa. Red - loading control, ab8245, observed at 37 kDa.

This western blot image is a comparison between ab11723 and a competitor's top cited rabbit polyclonal antibody.

All lanes:

Western blot - Anti-PKC alpha antibody [133] (ab11723)

Predicted band size: 76 kDa

false

• WB

Lab

Western blot - Anti-PKC alpha antibody [133] (AB11723)

5% NFDM/TBST

All lanes:

Western blot - Anti-PKC alpha antibody [133] (ab11723) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC delta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 76 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-PKC alpha antibody [133] (AB11723)

5% NFDM/TBST

All positive controls 10 ng

All lanes:

Western blot - Anti-PKC alpha antibody [133] (ab11723) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC eta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 5:

Western blot - Recombinant human PKC epsilon protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 6:

Western blot - Recombinant human PKC theta/PRKCQ protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 7:

Western blot - Recombinant human PKC mu/PKD protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-mu-pkd-protein-ab60873'>ab60873</a>)

Lane 8:

Western blot - Recombinant human PKC zeta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-zeta-protein-ab60848'>ab60848</a>)

Lane 9:

Western blot - Recombinant human PKC iota protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-iota-protein-ab60850'>ab60850</a>)

Lane 10:

Western blot - Recombinant human PKC gamma protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 76 kDa

false