Anti-PKC alpha antibody [133] - BSA and Azide free (ab284403)

Mouse Recombinant Monoclonal KPCA antibody. Carrier free. Suitable for WB, IHC-P and reacts with Recombinant full length protein - Human, Mouse, Rat, Human samples.

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Images

Western blot - Anti-PKC alpha antibody [133] - BSA and Azide free (AB284403), expandable thumbnail

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF
Human	Tested	Tested	Not recommended
Mouse	Tested	Tested	Not recommended
Rat	Tested	Tested	Not recommended
Recombinant full length protein - Human	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Recombinant full length protein - Human, Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant full length protein - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat, Mouse, Recombinant full length protein - Human	Dilution info -	Notes -

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

See full target information PRKCA

Function

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Exhibits anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, and in leukemia cells mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimulating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modulation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. During chemokine-induced CD4(+) T cell migration, phosphorylates CDC42-guanine exchange factor DOCK8 resulting in its dissociation from LRCH1 and the activation of GTPase CDC42 (PubMed:28028151). Is highly expressed in a number of cancer cells where it can act as a tumor promoter and is implicated in malignant phenotypes of several tumors such as gliomas and breast cancers. Negatively regulates myocardial contractility and positively regulates angiogenesis, platelet aggregation and thrombus formation in arteries. Mediates hypertrophic growth of neonatal cardiomyocytes, in part through a MAPK1/3 (ERK1/2)-dependent signaling pathway, and upon PMA treatment, is required to induce cardiomyocyte hypertrophy up to heart failure and death, by increasing protein synthesis, protein-DNA ratio and cell surface area. Regulates cardiomyocyte function by phosphorylating cardiac troponin T (TNNT2/CTNT), which induces significant reduction in actomyosin ATPase activity, myofilament calcium sensitivity and myocardial contractility. In angiogenesis, is required for full endothelial cell migration, adhesion to vitronectin (VTN), and vascular endothelial growth factor A (VEGFA)-dependent regulation of kinase activation and vascular tube formation. Involved in the stabilization of VEGFA mRNA at post-transcriptional level and mediates VEGFA-induced cell proliferation. In the regulation of calcium-induced platelet aggregation, mediates signals from the CD36/GP4 receptor for granule release, and activates the integrin heterodimer ITGA2B-ITGB3 through the RAP1GAP pathway for adhesion. During response to lipopolysaccharides (LPS), may regulate selective LPS-induced macrophage functions involved in host defense and inflammation. But in some inflammatory responses, may negatively regulate NF-kappa-B-induced genes, through IL1A-dependent induction of NF-kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1, which modulates EIF4G1 binding to MKNK1 and may be involved in the regulation of EIF4E phosphorylation. Phosphorylates KIT, leading to inhibition of KIT activity. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription. Phosphorylates SOCS2 at 'Ser-52' facilitating its ubiquitination and proteasomal degradation (By similarity). Phosphorylates KLHL3 in response to angiotensin II signaling, decreasing the interaction between KLHL3 and WNK4 (PubMed:25313067). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed:36040231).

Alternative names

PKCA, PRKACA, PRKCA, Protein kinase C alpha type, PKC-A, PKC-alpha

Recommended products

Mouse Recombinant Monoclonal KPCA antibody. Carrier free. Suitable for WB, IHC-P and reacts with Recombinant full length protein - Human, Mouse, Rat, Human samples.

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: 133
Purification technique: Affinity purification Protein A
Specificity: ab11723 recognises the alpha isoform of PKC.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

This product has switched from a hybridoma to recombinant production method on 25^th July 2021.

ab284403 is the carrier-free version of Anti-PKC alpha antibody [133] ab11723.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PKC alpha known as Protein Kinase C alpha is a serine/threonine-specific protein kinase involved in various cellular processes. The molecular mass of PKC alpha is approximately 80 kDa. Alternately called PKCA or PRKC alpha it is expressed in many tissues with high expression in the brain heart and lungs. PKC alpha plays a role in signal transduction by phosphorylating serine and threonine residues on target proteins.

Biological function summary

PKC alpha regulates cellular functions including proliferation differentiation and apoptosis. It often acts as part of a larger protein complex modulating various biological responses by interacting with substrates and co-factors. Its activity is dependent on calcium ions and diacylglycerol emphasizing its role in calcium and lipid signaling pathways.

Pathways

PKC alpha plays a vital role in the MAPK/ERK pathway and the PI3K/AKT pathway which are critical for the cellular stress response and growth regulation. PKC alpha interacts with several proteins in these pathways including RAF1 and AKT1 facilitating signal transmission that influences cell fate decisions.

Associated diseases and disorders

PKC alpha has been implicated in cancer and cardiac hypertrophy. In cancers aberrant PKC alpha signaling is linked to increased cell proliferation and survival. In cardiac hypertrophy it is associated with abnormal heart growth due to impaired signaling involving AKT1 and GSK3B. These connections highlight PKC alpha's influence in pathological as well as physiological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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9 product images

Western blot - Anti-PKC alpha antibody [133] - BSA and Azide free (ab284403)
This data was developed using Anti-PKC alpha antibody [133] ab11723 the same antibody clone in a different buffer formulation.
This image was generated from the hybridoma version of the product.
Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 μg)
Lanes 2, 6 and 10: PKC alpha knockout HAP1 cell lysate (20 μg)
Lanes 3, 7 and 11: K562 cell lysate (20 μg)
Lanes 4, 8 and 12: HEK293 cell lysate (20 μg)
Lanes 1, 2, 3 and 4: Green signal from target – Anti-PKC alpha antibody [133] ab11723 observed at 77 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control – Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signal
Anti-PKC alpha antibody [133] ab11723 was shown to specifically react with PKC alpha in wild-type HAP1 cells. No band was observed when PKC alpha knockout samples were examined. Wild-type and PKC alpha knockout samples were subjected to SDS-PAGE. Anti-PKC alpha antibody [133] ab11723 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/500 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PKC alpha antibody [133] - BSA and Azide free (ab284403)
Predicted band size: 76 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [133] - BSA and Azide free (ab284403)
This data was developed using Anti-PKC alpha antibody [133] ab11723 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of Paraffin-embedded human retina tissue labelling PKC alpha with Anti-PKC alpha antibody [133] ab11723 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on human retina is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) Goat Anti-Mouse IgG H&L (HRP polymer) ab214879.
Western blot - Anti-PKC alpha antibody [133] - BSA and Azide free (ab284403)
This data was developed using Anti-PKC alpha antibody [133] ab11723 the same antibody clone in a different buffer formulation.
This image was generated from the hybridoma version of the product.
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: PKC alpha knockout HAP1 cell lysate (20 μg)
Lane 3: K562 cell lysate (20 μg)
Lane 4: HEK293 cell lysate (20 μg)

Lanes 1 - 4: Merged signal (red and green). Green - Anti-PKC alpha antibody [133] ab11723 observed at 77 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
This western blot image is a comparison between Anti-PKC alpha antibody [133] ab11723 and a competitor's top cited rabbit polyclonal antibody.
All lanes: Western blot - Anti-PKC alpha antibody [133] - BSA and Azide free (ab284403)
Predicted band size: 76 kDa
Western blot - Anti-PKC alpha antibody [133] - BSA and Azide free (ab284403)
This data was developed using Anti-PKC alpha antibody [133] ab11723 the same antibody clone in a different buffer formulation.
5% NFDM/TBST
All positive controls 10 ng
All lanes: Western blot - Anti-PKC alpha antibody [133] (Anti-PKC alpha antibody [133] ab11723) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (Recombinant human PKC alpha protein (Active) ab55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (Recombinant human PKC beta 1 protein ab60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (Recombinant human PKC beta 2 protein ab60841)
Lane 4: Western blot - Recombinant human PKC eta protein (Recombinant human PKC eta protein ab60849)
Lane 5: Western blot - Recombinant human PKC epsilon protein (Recombinant human PKC epsilon protein ab60847)
Lane 6: Western blot - Recombinant human PKC theta/PRKCQ protein (Recombinant human PKC theta/PRKCQ protein ab56641)
Lane 7: Western blot - Recombinant human PKC mu/PKD protein (Recombinant human PKC mu/PKD protein ab60873)
Lane 8: Western blot - Recombinant human PKC zeta protein (Recombinant human PKC zeta protein ab60848)
Lane 9: Western blot - Recombinant human PKC iota protein (Recombinant human PKC iota protein ab60850)
Lane 10: Western blot - Recombinant human PKC gamma protein (Recombinant human PKC gamma protein ab60842)
Secondary
All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 76 kDa
Western blot - Anti-PKC alpha antibody [133] - BSA and Azide free (ab284403)
This data was developed using Anti-PKC alpha antibody [133] ab11723 the same antibody clone in a different buffer formulation.
5% NFDM/TBST
All lanes: Western blot - Anti-PKC alpha antibody [133] - BSA and Azide free (ab284403) at 1/1000 dilution
Lane 1: Recombinant human PKC alpha protein (Active) ab55672, 5 ng
Lane 2: Recombinant human PKC delta protein ab60844, 60 ng
Secondary
All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 76 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [133] - BSA and Azide free (ab284403)
This data was developed using Anti-PKC alpha antibody [133] ab11723 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse retina tissue labelling PKC alpha with Anti-PKC alpha antibody [133] ab11723 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on mouse retina is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) Goat Anti-Mouse IgG H&L (HRP polymer) ab214879.
Western blot - Anti-PKC alpha antibody [133] - BSA and Azide free (ab284403)
This data was developed using Anti-PKC alpha antibody [133] ab11723 the same antibody clone in a different buffer formulation.
5% NFDM/TBST
All lanes: Western blot - Anti-PKC alpha antibody [133] - BSA and Azide free (ab284403) at 1/1000 dilution
Lane 1: Human kidney tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: Rat spleen tissue lysate at 20 µg
Secondary
All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 76 kDa
Western blot - Anti-PKC alpha antibody [133] - BSA and Azide free (ab284403)
This data was developed using Anti-PKC alpha antibody [133] ab11723 the same antibody clone in a different buffer formulation.
Exposure time
Lane 1: 136 seconds
Lane 2 and 3: 15 seconds
Fresh lysates were used in lane 2 and 3.
All lanes: Western blot - Anti-PKC alpha antibody [133] - BSA and Azide free (ab284403) at 1/1000 dilution
Lane 1: C6 (rat glial tumor glial cell), whole cell lysate at 20 µg
Lane 2: HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
Secondary
All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 76 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [133] - BSA and Azide free (ab284403)
This data was developed using Anti-PKC alpha antibody [133] ab11723 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat retina tissue labelling PKC alpha with Anti-PKC alpha antibody [133] ab11723 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on rat retina is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) Goat Anti-Mouse IgG H&L (HRP polymer) ab214879.