Rabbit Recombinant Monoclonal KPCA phospho S657 antibody. Suitable for IHC-P, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide, Recombinant full length protein - Human samples. Cited in 21 publications.
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IHC-P | Dot | WB | |
---|---|---|---|
Human | Tested | Expected | Tested |
Mouse | Expected | Expected | Tested |
Rat | Expected | Expected | Tested |
Recombinant full length protein - Human | Not recommended | Not recommended | Tested |
Synthetic peptide | Not recommended | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/5000 | Notes The use of an HRP/AP polymerized secondary antibody is recommended. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified use at 1/1500. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/5000 | Notes The use of an HRP/AP polymerized secondary antibody is recommended. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified use at 1/1500. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/5000 | Notes The use of an HRP/AP polymerized secondary antibody is recommended. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. For unpurified use at 1/1500. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide, Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species Recombinant full length protein - Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
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Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Exhibits anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, and in leukemia cells mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimulating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modulation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. During chemokine-induced CD4(+) T cell migration, phosphorylates CDC42-guanine exchange factor DOCK8 resulting in its dissociation from LRCH1 and the activation of GTPase CDC42 (PubMed:28028151). Is highly expressed in a number of cancer cells where it can act as a tumor promoter and is implicated in malignant phenotypes of several tumors such as gliomas and breast cancers. Negatively regulates myocardial contractility and positively regulates angiogenesis, platelet aggregation and thrombus formation in arteries. Mediates hypertrophic growth of neonatal cardiomyocytes, in part through a MAPK1/3 (ERK1/2)-dependent signaling pathway, and upon PMA treatment, is required to induce cardiomyocyte hypertrophy up to heart failure and death, by increasing protein synthesis, protein-DNA ratio and cell surface area. Regulates cardiomyocyte function by phosphorylating cardiac troponin T (TNNT2/CTNT), which induces significant reduction in actomyosin ATPase activity, myofilament calcium sensitivity and myocardial contractility. In angiogenesis, is required for full endothelial cell migration, adhesion to vitronectin (VTN), and vascular endothelial growth factor A (VEGFA)-dependent regulation of kinase activation and vascular tube formation. Involved in the stabilization of VEGFA mRNA at post-transcriptional level and mediates VEGFA-induced cell proliferation. In the regulation of calcium-induced platelet aggregation, mediates signals from the CD36/GP4 receptor for granule release, and activates the integrin heterodimer ITGA2B-ITGB3 through the RAP1GAP pathway for adhesion. During response to lipopolysaccharides (LPS), may regulate selective LPS-induced macrophage functions involved in host defense and inflammation. But in some inflammatory responses, may negatively regulate NF-kappa-B-induced genes, through IL1A-dependent induction of NF-kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1, which modulates EIF4G1 binding to MKNK1 and may be involved in the regulation of EIF4E phosphorylation. Phosphorylates KIT, leading to inhibition of KIT activity. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription. Phosphorylates SOCS2 at 'Ser-52' facilitating its ubiquitination and proteasomal degradation (By similarity). Phosphorylates KLHL3 in response to angiotensin II signaling, decreasing the interaction between KLHL3 and WNK4 (PubMed:25313067). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed:36040231).
PKCA, PRKACA, PRKACA, PKCA, PRKCA, Protein kinase C alpha type, PKC-A, PKC-alpha
Rabbit Recombinant Monoclonal KPCA phospho S657 antibody. Suitable for IHC-P, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide, Recombinant full length protein - Human samples. Cited in 21 publications.
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR1901(2)
Affinity purification Protein A
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
ab180848 detects various PKC phosphorylated isoforms
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
PKC alpha known as Protein Kinase C alpha is a serine/threonine-specific protein kinase involved in various cellular processes. The molecular mass of PKC alpha is approximately 80 kDa. Alternately called PKCA or PRKC alpha it is expressed in many tissues with high expression in the brain heart and lungs. PKC alpha plays a role in signal transduction by phosphorylating serine and threonine residues on target proteins.
PKC alpha regulates cellular functions including proliferation differentiation and apoptosis. It often acts as part of a larger protein complex modulating various biological responses by interacting with substrates and co-factors. Its activity is dependent on calcium ions and diacylglycerol emphasizing its role in calcium and lipid signaling pathways.
PKC alpha plays a vital role in the MAPK/ERK pathway and the PI3K/AKT pathway which are critical for the cellular stress response and growth regulation. PKC alpha interacts with several proteins in these pathways including RAF1 and AKT1 facilitating signal transmission that influences cell fate decisions.
PKC alpha has been implicated in cancer and cardiac hypertrophy. In cancers aberrant PKC alpha signaling is linked to increased cell proliferation and survival. In cardiac hypertrophy it is associated with abnormal heart growth due to impaired signaling involving AKT1 and GSK3B. These connections highlight PKC alpha's influence in pathological as well as physiological conditions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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All lanes: Western blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848) at 1/1000 dilution
Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg
Lane 2: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 77 kDa
This data was developed using ab180848, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue sections labeling PKC alpha with purified ab180848 at 1:5000 (0.23 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. Postive staining on human prostatic hyperplasia without alkaline phosphatase treatment (image A). No staining on human prostatic hyperplasia with alkaline phosphatase treatment (image B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Dot blot analysis of PKC alpha (pS657) phospho peptide (Lane 1) and PKC alpha non-phospho peptide (Lane 2) using ab180848 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution.
Blocking and Diluting buffer and concentration: 5% NFDM /TBST.
Exposure time: 10 seconds.
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848) at 1/10000 dilution
Lane 1: Untreated C6 (rat glioma) whole cell lysate at 15 µg
Lane 2: C6 (rat glioma) treated with 80nM Phorbol-12-myristate-13-acetate, whole cell lysate at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 77 kDa
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848) at 1/10000 dilution
Lane 1: Untreated Jurkat (human acute T cell leukemia) whole cell lysate at 15 µg
Lane 2: Jurkat (human acute T cell leukemia) treated with 200ng/ml phorbol-12,13-dibutyrate for 45 minutes, whole cell lysate at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 77 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (Recombinant human PKC alpha protein (Active) ab55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (Recombinant human PKC beta 1 protein ab60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (Recombinant human PKC beta 2 protein ab60841)
Lane 4: Western blot - Recombinant human PKC gamma protein (Recombinant human PKC gamma protein ab60842)
Lane 5: Western blot - Recombinant human PKC delta protein (Recombinant human PKC delta protein ab60844)
Lane 6: Western blot - Recombinant human PKC epsilon protein (Recombinant human PKC epsilon protein ab60847)
Lane 7: Western blot - Recombinant human PKC zeta protein (Recombinant human PKC zeta protein ab60848)
Lane 8: Western blot - Recombinant human PKC eta protein (Recombinant human PKC eta protein ab60849)
Lane 9: Western blot - Recombinant human PKC theta/PRKCQ protein (Recombinant human PKC theta/PRKCQ protein ab56641)
Lane 10: Western blot - Recombinant human PKC iota protein (Recombinant human PKC iota protein ab60850)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 105 kDa
Exposure time: 60s
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