Anti-PKC antibody [EPR16898]
- RabMAb
- Recombinant
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(10 Publications)
Rabbit Recombinant Monoclonal KPCB antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Mouse, Human, Recombinant full length protein - Human samples. Cited in 10 publications.
View Alternative Names
PKCB, PRKCB1, PRKCB, Protein kinase C beta type, PKC-B, PKC-beta
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-PKC antibody [EPR16898] (AB179522)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling PKC alpha + beta + gamma with ab179522 at 1/200 dilution, followed by Goat anti-rabbit IAlexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image shows cytoplasmic staining on K562 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution (red).
The negative controls are as follows : -ve control 1 : ab179522 at 1/200 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-PKC antibody [EPR16898] (AB179522)
Intracellular flow cytometric analysis of2% paraformaldehyde-fixed NIH/3T3 (Mouse embyro fibroblast cells) cells labeling PKC alpha + beta + gamma with ab179522 at 1/360 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
- IP
Supplier Data
Immunoprecipitation - Anti-PKC antibody [EPR16898] (AB179522)
PKC alpha + beta + gamma was immunoprecipitated from 1mg of Rat brain extract with ab179522 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab179522 at 1/1000 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody was used as secondary antibody at 1/1000 dilution.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-PKC antibody [EPR16898] (ab179522)
Predicted band size: 77 kDa
false
- WB
Lab
Western blot - Anti-PKC antibody [EPR16898] (AB179522)
Active human PKC zeta full length protein (Catalog# ab60848) contains aa1-592 with GST-tag; Active human PKC iota full length protein (Catalog#ab60850) contains aa1-596 with GST-tag.
Blocking/Diluting buffer 5% NFDM/TBST
All lanes:
Western blot - Anti-PKC antibody [EPR16898] (ab179522) at 1/2000 dilution
Lane 1:
Active human PKC zeta full length protein at 0.02 µg
Lane 2:
Active human PKC iota full length protein at 0.02 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
false
Exposure time: 3min
- WB
Supplier Data
Western blot - Anti-PKC antibody [EPR16898] (AB179522)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-PKC antibody [EPR16898] (ab179522) at 1/1000 dilution
Lane 1:
Human fetal brain tissue lysate at 10 µg
Lane 2:
Human fetal heart tissue lysate at 10 µg
Lane 3:
Human fetal kidney tissue lysate at 10 µg
Secondary
All lanes:
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDa
false
- WB
Supplier Data
Western blot - Anti-PKC antibody [EPR16898] (AB179522)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-PKC antibody [EPR16898] (ab179522) at 1/10000 dilution
Lane 1:
HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2:
293T (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg
Lane 3:
SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDa
false
- WB
Lab
Western blot - Anti-PKC antibody [EPR16898] (AB179522)
Active human PKC alpha full length protein (Catalog#ab55672) contains aa1-672 with GST-tag; Active human PKC beta 1 full length protein(Catalog#ab60840) contains aa1-671 with GST-tag; Active human PKC beta 2 full length protein(Catalog#ab60841) contains aa1-673 with GST-tag; Active human PKC gamma full length protein(Catalog#ab60842) contains aa1-677 with GST-tag; Active human PKC delta full length protein(Catalog#ab60844) contains aa1-676 with GST-tag; Active human PKC eta full length protein(Catalog#ab60849) contains aa1-683 with GST-tag; Active human PKC epsilon full length protein(Catalog#ab60847) contains aa1-737 with GST-tag; Active human PKC theta full length protein(Catalog#ab56641) contains aa1-706 with GST-tag; Active human PKC mu full length protein(Catalog#ab60873) contains aa1-912 with GST-tag.
Blocking/Diluting buffer 5% NFDM/TBST
All lanes:
Western blot - Anti-PKC antibody [EPR16898] (ab179522) at 1/2000 dilution
Lane 1:
Active human PKC alpha full length protein at 0.02 µg
Lane 2:
Active human PKC beta 1 full length protein at 0.02 µg
Lane 3:
Active human PKC beta 2 full length protein at 0.02 µg
Lane 4:
Active human PKC gamma full length protein at 0.02 µg
Lane 5:
Active human PKC delta full length protein at 0.02 µg
Lane 6:
Active human PKC eta full length protein at 0.02 µg
Lane 7:
Active human PKC epsilon full length protein at 0.02 µg
Lane 8:
Active human PKC theta full length protein at 0.02 µg
Lane 9:
Active human PKC mu full length protein at 0.02 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Predicted band size: 77 kDa
false
Exposure time: 2s
- WB
Supplier Data
Western blot - Anti-PKC antibody [EPR16898] (AB179522)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-PKC antibody [EPR16898] (ab179522) at 1/1000 dilution
Lane 1:
K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg
Lane 2:
A431 (Human epidermoid carcinoma) whole cell lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDa
false
- WB
Supplier Data
Western blot - Anti-PKC antibody [EPR16898] (AB179522)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-PKC antibody [EPR16898] (ab179522) at 1/5000 dilution
Lane 1:
L6 (Rat skeletal muscle cell line) whole cell lysate at 10 µg
Lane 2:
Neuro-2a (Mouse neuroblastoma cells) whole cell lysate at 10 µg
Lane 3:
L-929 (Mouse connective tissue fibroblast cells) whole cell lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDa
false
- WB
Supplier Data
Western blot - Anti-PKC antibody [EPR16898] (AB179522)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-PKC antibody [EPR16898] (ab179522) at 1/1000 dilution
All lanes:
Rat brain whole tissue lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDa
false
- WB
Unknown
Western blot - Anti-PKC antibody [EPR16898] (AB179522)
Recombinant PKC isoforms were tested for reactivity by western blot, and are marked as positive (+) or negative (-).
All lanes:
Western blot - Anti-PKC antibody [EPR16898] (ab179522)
false
Related conjugates and formulations (1)
-
Anti-PKC antibody [EPR16898] - BSA and Azide free
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PKC influences various cellular processes such as cell proliferation differentiation apoptosis and immune responses. It acts within larger protein complexes serving as a modulator of cellular functions. The activation of PKC leads to its association with different scaffolding proteins impacting a wide array of cell activities. PKC notably affects the function of other kinases and transcription factors demonstrating its integral role in managing cellular behavior and homeostasis.
Pathways
PKC participates in the phosphoinositide signaling pathway and the mitogen-activated protein kinase (MAPK) pathway. In these contexts PKC activation impacts proteins like Ras and Raf kinases which play roles in cellular growth and differentiation. By modulating these pathways PKC becomes an important component in signal transduction cascades influencing cell fate decisions. Understanding the interaction between PKC and these pathways helps in elucidating its role in maintaining normal cellular activities and responses to external stimuli.
Product protocols
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Target data
Additional targets
Publications (10)
Recent publications for all applications. Explore the full list and refine your search
Nature communications 13:3301 PubMed35676256
2022
Applications
Unspecified application
Species
Unspecified reactive species
Theranostics 11:9089-9106 PubMed34522228
2021
Applications
Unspecified application
Species
Unspecified reactive species
Journal of biochemical and molecular toxicology 34:e22493 PubMed32319158
2020
Applications
Unspecified application
Species
Unspecified reactive species
General and comparative endocrinology 288:113371 PubMed31857076
2019
Applications
Unspecified application
Species
Unspecified reactive species
American journal of physiology. Renal physiology 318:F457-F467 PubMed31760768
2019
Applications
Unspecified application
Species
Unspecified reactive species
Diabetes, metabolic syndrome and obesity : targets 12:2289-2302 PubMed31807042
2019
Applications
Unspecified application
Species
Unspecified reactive species
Biochemistry and biophysics reports 13:99-108 PubMed29556564
2018
Applications
Unspecified application
Species
Unspecified reactive species
Oncotarget 8:88630-88644 PubMed29179463
2017
Applications
WB
Species
Mouse
Cell death & disease 7:e2487 PubMed27882945
2016
Applications
WB
Species
Mouse
The Journal of neuroscience : the official journal of the Society for Neuroscience 36:6393-402 PubMed27307229
2016
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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