Anti-PKC beta 2 antibody [Y125]

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-PKC beta 2 antibody [Y125] (AB32026)

Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling PKC beta 2 with purified ab32026 at 1/20 dilution (5 ug/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as a isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC beta 2 antibody [Y125] (AB32026)

Immunohistochemical analysis of Paraffin-embedded sections human gastric carcinoma tissue labelling PKC beta 2 with ab32026 at 1/300 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on human gastric carcinoma tissue is observed. Counter stained with Haematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PKC beta 2 antibody [Y125] (AB32026)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PKC beta 2 using ab32026. The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% Triton X-100. The cells were then incubated with ab32026 at 1 : 50 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1 : 200 dilution (shown in red). Secondary antibody only control : PBS instead of the primary antibody.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC beta 2 antibody [Y125] (AB32026)

Immunohistochemical analysis of Paraffin-embedded sections mouse cerebrum tissue labelling PKC beta 2 with ab32026 at 1/1000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on mouse cerebrum tissue is observed. Counter stained with Haematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC beta 2 antibody [Y125] (AB32026)

Immunohistochemical analysis of Paraffin-embedded sections rat cerebrum tissue labelling PKC beta 2 with ab32026 at 1/1000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on rat cerebrum tissue is observed. Counter stained with Haematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• WB

Lab

Western blot - Anti-PKC beta 2 antibody [Y125] (AB32026)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as GAPDH loading control. We recommend not to boil samples when loading lysate.

All lanes:

Western blot - Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/1000 dilution

Lane 1:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate boiled at 20 µg

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate unboiled at 20 µg

Lane 3:

Mouse brain lysate boiled at 20 µg

Lane 4:

Mouse brain lysate unboiled at 20 µg

Lane 5:

Rat brain lysate boiled at 20 µg

Lane 6:

Rat brain lysate unboiled at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 77 kDa

Observed band size: 77 kDa

false

• WB

Lab

Western blot - Anti-PKC beta 2 antibody [Y125] (AB32026)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab235384 was used to detect GST tag.

All lanes:

Western blot - Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC beta 2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>) at 10 ng

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>) at 10 ng

Lane 3:

Western blot - Recombinant human PKC delta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>) at 10 ng

Lane 4:

Western blot - Recombinant human PKC eta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>) at 10 ng

Lane 5:

Western blot - Recombinant human PKC epsilon protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>) at 10 ng

Lane 6:

Western blot - Recombinant human PKC theta/PRKCQ protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>) at 10 ng

Lane 7:

Western blot - Recombinant human PKC mu/PKD protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-mu-pkd-protein-ab60873'>ab60873</a>) at 10 ng

Lane 8:

Western blot - Recombinant human PKC alpha protein (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>) at 10 ng

Lane 9:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 77 kDa

Observed band size: 100 kDa

false