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Rabbit Recombinant Monoclonal PKC theta/PRKCQ antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (AB250576), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (AB250576), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (AB250576), expandable thumbnail
  • Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (AB250576), expandable thumbnail
  • Western blot - Anti-PKC delta + PKC theta antibody [EPR17074] - BSA and Azide free (AB250576), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Rat
Tested
Expected
Tested

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that mediates non-redundant functions in T-cell receptor (TCR) signaling, including T-cells activation, proliferation, differentiation and survival, by mediating activation of multiple transcription factors such as NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated T-cells, is required for the activation of NF-kappa-B and JUN, which in turn are essential for IL2 production, and participates in the calcium-dependent NFATC1 and NFATC2 transactivation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11 on several serine residues, inducing CARD11 association with lipid rafts and recruitment of the BCL10-MALT1 complex, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May also play an indirect role in activation of the non-canonical NF-kappa-B (NFKB2) pathway. In the signaling pathway leading to JUN activation, acts by phosphorylating the mediator STK39/SPAK and may not act through MAP kinases signaling. Plays a critical role in TCR/CD28-induced NFATC1 and NFATC2 transactivation by participating in the regulation of reduced inositol 1,4,5-trisphosphate generation and intracellular calcium mobilization. After costimulation of T-cells through CD28 can phosphorylate CBLB and is required for the ubiquitination and subsequent degradation of CBLB, which is a prerequisite for the activation of TCR. During T-cells differentiation, plays an important role in the development of T-helper 2 (Th2) cells following immune and inflammatory responses, and, in the development of inflammatory autoimmune diseases, is necessary for the activation of IL17-producing Th17 cells. May play a minor role in Th1 response. Upon TCR stimulation, mediates T-cell protective survival signal by phosphorylating BAD, thus protecting T-cells from BAD-induced apoptosis, and by up-regulating BCL-X(L)/BCL2L1 levels through NF-kappa-B and JUN pathways. In platelets, regulates signal transduction downstream of the ITGA2B, CD36/GP4, F2R/PAR1 and F2RL3/PAR4 receptors, playing a positive role in 'outside-in' signaling and granule secretion signal transduction. May relay signals from the activated ITGA2B receptor by regulating the uncoupling of WASP and WIPF1, thereby permitting the regulation of actin filament nucleation and branching activity of the Arp2/3 complex. May mediate inhibitory effects of free fatty acids on insulin signaling by phosphorylating IRS1, which in turn blocks IRS1 tyrosine phosphorylation and downstream activation of the PI3K/AKT pathway. Phosphorylates MSN (moesin) in the presence of phosphatidylglycerol or phosphatidylinositol. Phosphorylates PDPK1 at 'Ser-504' and 'Ser-532' and negatively regulates its ability to phosphorylate PKB/AKT1. Phosphorylates CCDC88A/GIV and inhibits its guanine nucleotide exchange factor activity (PubMed:23509302).

Targets

PRKCD

Alternative names

Protein kinase C theta type, nPKC-theta, PRKCQ, PRKCT

Recommended products

Rabbit Recombinant Monoclonal PKC theta/PRKCQ antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples.

Alternative names

Protein kinase C theta type, nPKC-theta, PRKCQ, PRKCT

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR17074

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab250576 is the carrier-free version of Anti-PKC delta + PKC theta antibody [EPR17074] ab182127.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PKC delta (Protein Kinase C delta) and PKC theta (Protein Kinase C theta) are members of the Protein Kinase C (PKC) family that function as serine/threonine kinases. These proteins with masses of approximately 78 kDa for PKC delta and 82 kDa for PKC theta play key roles in intracellular signaling processes. PKC delta is expressed in various tissues with particular abundance in heart lung and muscle. PKC theta is mainly found in T cells and skeletal muscle. Both proteins require calcium and diacylglycerol for activation and are often associated with lipid membranes where they exert their enzymatic activity.

Biological function summary

PKC delta and PKC theta regulate diverse cellular processes including proliferation differentiation and apoptosis. PKC delta often participates in programmed cell death and is a part of several signaling cascades initiated by external stress signals. PKC theta is largely recognized for its function in immune response regulation particularly in T cell activation. These proteins are critical in forming signaling complexes with other molecules like scaffold proteins which helps in propagating their functional roles within the cell.

Pathways

PKC delta and PKC theta are integral components of the MAPK (Mitogen-Activated Protein Kinase) and NF-kB (Nuclear Factor kappa-light-chain-enhancer of activated B cells) pathways. In the MAPK pathway PKC delta interacts significantly with proteins such as ERK and MEK mediating cell growth responses. PKC theta activates the NF-kB pathway in lymphocytes enhancing immune response and involving interaction with proteins like IkB kinase (IKK). These contributions show their impact on cellular events through precise regulation of signaling pathways.

Associated diseases and disorders

PKC delta and PKC theta are linked to cancer and autoimmune diseases. PKC delta's regulation of apoptosis makes it a significant player in tumor suppression with alterations in its activity observed in several types of cancer. In autoimmune diseases such as rheumatoid arthritis PKC theta's role in T cell activity and cytokine production connects it to dysregulated immune responses. Both proteins interact with tumor suppressor p53 in cancer contexts revealing their importance in maintaining cellular equilibrium and responses to dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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