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Rabbit Recombinant Monoclonal KPCL antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 6 publications.


Images

Immunoprecipitation - Anti-PKC eta antibody [EPR18513] (AB179524), expandable thumbnail
  • Western blot - Anti-PKC eta antibody [EPR18513] (AB179524), expandable thumbnail
  • Western blot - Anti-PKC eta antibody [EPR18513] (AB179524), expandable thumbnail
  • Western blot - Anti-PKC eta antibody [EPR18513] (AB179524), expandable thumbnail
  • Western blot - Anti-PKC eta antibody [EPR18513] (AB179524), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Expected
Tested
Mouse
Tested
Expected
Rat
Expected
Tested

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/2000

Notes

-

Species

Human

Dilution info

1/2000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-CDKN1B complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1-dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Phosphorylates ATF2 which promotes its nuclear retention and transcriptional activity and negatively regulates its mitochondrial localization.

Alternative names

Protein kinase C eta type, PKC-L, nPKC-eta, PRKCL, PKCL, PRKCH

Recommended products

Rabbit Recombinant Monoclonal KPCL antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 6 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR18513

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Protein kinase C eta (PKC eta) is an enzyme also known as PKC η which belongs to the PKC family of serine/threonine kinases. This protein has a molecular mass of approximately 80 kDa and shows expression in numerous tissues with strong presence in epithelia and the immune system. PKC eta's mechanical function involves phosphorylation of serine and threonine residues on cellular target proteins modulating their activity. It plays a significant role in signal transduction pathways that govern various cellular processes.

Biological function summary

PKC eta involves itself in cellular differentiation and proliferation. It is a component of signal transduction complexes that mediate these processes. In epithelial cells PKC eta modulates cell adhesion and integrity. It also affects immune responses through its action in T-cells and other immune components impacting both innate and adaptive immunity. The enzyme interacts closely with other PKC isoforms allowing it to regulate diverse biological functions through complex networks.

Pathways

PKC eta plays an integral role in growth factor and cytokine signaling pathways. MAPK/ERK and PI3K/AKT pathways include PKC eta as key components for cell survival and proliferation. These pathways facilitate interactions and regulation involving proteins like RAF-1 and AKT coordinating cell growth and apoptosis. PKC eta's positioning in these pathways denotes its significance in maintaining cellular homeostasis and responding to external stimuli.

Associated diseases and disorders

PKC eta implicates itself in autoimmune conditions like rheumatoid arthritis and in certain types of cancer such as breast cancer. PKC eta influences inflammatory responses often exacerbating autoimmune phenomena by interacting with cytokines and immune receptors. In cancer it may contribute to tumorigenesis by promoting cell survival through PI3K/AKT pathway engagement. It connects with proteins like NF-kB driving pathological changes in cell behavior and immune modulation.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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7 product images

  • Immunoprecipitation - Anti-PKC eta antibody [EPR18513] (ab179524), expandable thumbnail

    Immunoprecipitation - Anti-PKC eta antibody [EPR18513] (ab179524)

    PKC eta was immunoprecipitated from 1mg of Mouse lung whole cell lysate with ab179524 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab179524 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Mouse lung whole cell lysate 10ug (Input).

    Lane 2: ab179524 IP in Mouse lung whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab179524 in Mouse lung whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-PKC eta antibody [EPR18513] (ab179524)

    Predicted band size: 78 kDa

    Observed band size: 40 kDa, 78 kDa

  • Western blot - Anti-PKC eta antibody [EPR18513] (ab179524), expandable thumbnail

    Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Active human PKC alpha full length protein (Recombinant human PKC alpha protein (Active) ab55672) contains aa1-672 with GST-tag; Active human PKC beta 1 full length protein (Recombinant human PKC beta 1 protein ab60840) contains aa1-671 with GST-tag; Active human PKC beta 2 full length protein (Recombinant human PKC beta 2 protein ab60841) contains aa1-673 with GST-tag; Active human PKC gamma full length protein(Recombinant human PKC gamma protein ab60842) contains aa1-677 with GST-tag; Active human PKC delta full length protein (Recombinant human PKC delta protein ab60844) contains aa1-676 with GST-tag; Active human PKC eta full length protein (Recombinant human PKC eta protein ab60849) contains aa1-683 with GST-tag; Active human PKC epsilon full length protein (Recombinant human PKC epsilon protein ab60847) contains aa1-737 with GST-tag; Active human PKC theta full length protein (Recombinant human PKC theta/PRKCQ protein ab56641) contains aa1-706 with GST-tag; Active human PKC mu full length protein (Recombinant human PKC mu/PKD protein ab60873) contains aa1-912 with GST-tag.

    All lanes: Western blot - Anti-PKC eta antibody [EPR18513] (ab179524) at 1/2000 dilution

    Lane 1: Active human PKC alpha full length protein at 0.02 µg

    Lane 2: Active human PKC beta 1 full length protein at 0.02 µg

    Lane 3: Active human PKC beta 2 full length protein at 0.02 µg

    Lane 4: Active human PKC gamma full length protein at 0.02 µg

    Lane 5: Active human PKC delta full length protein at 0.02 µg

    Lane 6: Active human PKC eta full length protein at 0.02 µg

    Lane 7: Active human PKC epsilon full length protein at 0.02 µg

    Lane 8: Active human PKC theta full length protein at 0.02 µg

    Lane 9: Active human PKC mu full length protein at 0.02 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa

    Exposure time: 1s

  • Western blot - Anti-PKC eta antibody [EPR18513] (ab179524), expandable thumbnail

    Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
    (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC, 1995. J. Biol. Chem. 270, 28495-28498)

    All lanes: Western blot - Anti-PKC eta antibody [EPR18513] (ab179524) at 1/2000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: Human fetal heart lysate at 10 µg

    Lane 3: Human fetal kidney lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 78 kDa

    Observed band size: 45 kDa, 78 kDa

    Exposure time: 3min

  • Western blot - Anti-PKC eta antibody [EPR18513] (ab179524), expandable thumbnail

    Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
    (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC. 1995. J. Biol. Chem. 270, 28495-28498)

    All lanes: Western blot - Anti-PKC eta antibody [EPR18513] (ab179524) at 1/10000 dilution

    Lane 1: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

    Lane 2: PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa

    Observed band size: 45 kDa, 78 kDa

    Exposure time: 1min

  • Western blot - Anti-PKC eta antibody [EPR18513] (ab179524), expandable thumbnail

    Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
    (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC, 1995. J. Biol. Chem. 270, 28495-28498)

    All lanes: Western blot - Anti-PKC eta antibody [EPR18513] (ab179524) at 1/10000 dilution

    Lane 1: Human fetal skin lysate at 10 µg

    Lane 2: Mouse lung lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa

    Observed band size: 45 kDa, 78 kDa

    Exposure time: 30s

  • Western blot - Anti-PKC eta antibody [EPR18513] (ab179524), expandable thumbnail

    Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
    (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC, 1995. J. Biol. Chem. 270, 28495-28498)

    All lanes: Western blot - Anti-PKC eta antibody [EPR18513] (ab179524) at 1/2000 dilution

    Lane 1: Rat skin lysate at 10 µg

    Lane 2: Mouse skin lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa

    Observed band size: 45 kDa, 78 kDa

    Exposure time: 15s

  • Western blot - Anti-PKC eta antibody [EPR18513] (ab179524), expandable thumbnail

    Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
    (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC, 1995. J. Biol. Chem. 270, 28495-28498)

    All lanes: Western blot - Anti-PKC eta antibody [EPR18513] (ab179524) at 1/10000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Rat brain lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa

    Observed band size: 45 kDa, 78 kDa

    Exposure time: 30s

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