Anti-PKC eta antibody [EPR18513] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-PKC eta antibody [EPR18513] - BSA and Azide free (AB240218)

PKC eta was immunoprecipitated from 1mg of Mouse lung whole cell lysate with ab179524 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab179524 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Mouse lung whole cell lysate 10ug (Input).

Lane 2 : ab179524 IP in Mouse lung whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab179524 in Mouse lung whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179524).

All lanes:

Immunoprecipitation - Anti-PKC eta antibody [EPR18513] (<a href='/en-us/products/primary-antibodies/pkc-eta-antibody-epr18513-ab179524'>ab179524</a>)

Predicted band size: 78 kDa

Observed band size: 40 kDa,78 kDa

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• WB

Lab

Western blot - Anti-PKC eta antibody [EPR18513] - BSA and Azide free (AB240218)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179524).

Western blot : Rabbit Monoclonal [EPR18513] to PKC ab179524 staining at 1/2000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 76, 85 kDa in Wild-type A549 cell lysates with no signal observed at this size in PRKCH knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^™ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PKC eta antibody [EPR18513] (<a href='/en-us/products/primary-antibodies/pkc-eta-antibody-epr18513-ab179524'>ab179524</a>) at 1/2000 dilution

Lane 1:

Wild-type A549 whole cell lysate at 20 µg

Lane 2:

PRKCH knockout A549 whole cell lysate at 20 µg

Lane 3:

U-2 OS whole cell lysate at 20 µg

Lane 4:

HL-60 whole cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 76 kDa,85 kDa

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