Rabbit Polyclonal KPCL phospho T655 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Protein kinase C eta type phospho T655.
IgG
Rabbit
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
Liquid
Polyclonal
WB | |
---|---|
Human | Tested |
Mouse | Predicted |
Rat | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.35000-1.00000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
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Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-CDKN1B complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1-dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Phosphorylates ATF2 which promotes its nuclear retention and transcriptional activity and negatively regulates its mitochondrial localization.
PKCL, PRKCL, PRKCL, PKCL, PRKCH, Protein kinase C eta type, PKC-L, nPKC-eta
Rabbit Polyclonal KPCL phospho T655 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Protein kinase C eta type phospho T655.
IgG
Rabbit
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
This antibody does not cross-react with any other PKC isoforms tested.
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKC eta. The final product is generated by affinity chromatography using a PKC eta-derived peptide that is phosphorylated at threonine 655.
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Protein kinase C eta (PKC eta) is an enzyme also known as PKC η which belongs to the PKC family of serine/threonine kinases. This protein has a molecular mass of approximately 80 kDa and shows expression in numerous tissues with strong presence in epithelia and the immune system. PKC eta's mechanical function involves phosphorylation of serine and threonine residues on cellular target proteins modulating their activity. It plays a significant role in signal transduction pathways that govern various cellular processes.
PKC eta involves itself in cellular differentiation and proliferation. It is a component of signal transduction complexes that mediate these processes. In epithelial cells PKC eta modulates cell adhesion and integrity. It also affects immune responses through its action in T-cells and other immune components impacting both innate and adaptive immunity. The enzyme interacts closely with other PKC isoforms allowing it to regulate diverse biological functions through complex networks.
PKC eta plays an integral role in growth factor and cytokine signaling pathways. MAPK/ERK and PI3K/AKT pathways include PKC eta as key components for cell survival and proliferation. These pathways facilitate interactions and regulation involving proteins like RAF-1 and AKT coordinating cell growth and apoptosis. PKC eta's positioning in these pathways denotes its significance in maintaining cellular homeostasis and responding to external stimuli.
PKC eta implicates itself in autoimmune conditions like rheumatoid arthritis and in certain types of cancer such as breast cancer. PKC eta influences inflammatory responses often exacerbating autoimmune phenomena by interacting with cytokines and immune receptors. In cancer it may contribute to tumorigenesis by promoting cell survival through PI3K/AKT pathway engagement. It connects with proteins like NF-kB driving pathological changes in cell behavior and immune modulation.
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Peptide Competition and Phosphatase Treatment Lysates prepared from Jurkat cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-9) or treated with lambda phosphatase (10), blocked with a 5% BSA-TBST buffer overnight at 4°C, and incubated with 0.50 μg/mL ab5798 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 10), the non phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), the phosphopeptide immunogen (4), or, the phosphopeptide corresponding to the immunogen from other PKC isoforms (5-9). After washing, membranes were incubated with goat (ab’)2 anti-rabbit IgG HRP-conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the peptide corresponding to PKC eta [pT655] blocks the antibody signal. The a
All lanes: Western blot - Anti-PKC eta (phospho T655) antibody (ab5798)
Predicted band size: 78 kDa
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