Anti-PKC gamma antibody [EPR2750]

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-PKC gamma antibody [EPR2750] (AB108961)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh frozen) tissue labeling PKC gamma with ab108961 at 1/100 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).

Negative control : Confocal image showing no staining on mouse kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with ab108961 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-PKC gamma antibody [EPR2750] (AB108961)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney (fresh frozen) tissue labeling PKC gamma with ab108961 at 1/100 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).

Negative control : Confocal image showing no staining on rat kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with ab108961 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-PKC gamma antibody [EPR2750] (AB108961)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh frozen) tissue labeling PKC gamma with ab108961 at 1/100 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing positive staining on rat cerebellum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab108961 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-PKC gamma antibody [EPR2750] (AB108961)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh frozen) tissue labeling PKC gamma with ab108961 at 1/100 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing positive staining on mouse cerebellum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab108961 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.

• WB

Lab

Western blot - Anti-PKC gamma antibody [EPR2750] (AB108961)

Blocking and Diluting buffer and concentration : 5% NFDM/TBST. Negative control : Human kidney, mouse kidney, mouse skin, rat skin, rat skeletal muscle. Samples are non-boiled as boiling may cause protein aggregation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-PKC gamma antibody [EPR2750] (ab108961) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 40 µg

Lane 2:

Human kidney tissue lysate at 40 µg

Lane 3:

Mouse brain tissue lysate at 40 µg

Lane 4:

Mouse cerebellum tissue lysate at 40 µg

Lane 5:

Mouse kidney tissue lysate at 40 µg

Lane 6:

Mouse skin tissue lysate at 40 µg

Lane 7:

Rat brain tissue lysate at 40 µg

Lane 8:

Rat skin tissue lysate at 40 µg

Lane 9:

Rat skeletal muscle tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 6s

• WB

Lab

Western blot - Anti-PKC gamma antibody [EPR2750] (AB108961)

Blocking and Diluting buffer and concentration : 5% NFDM/TBST. In Western blot, anti-GST antibody (ab111947) loading control staining at 1/1000 dilution. This antibody does not cross-react with human PKC beta 1, human PKC beta 2 and human PKC delta proteins.

All lanes:

Western blot - Anti-PKC gamma antibody [EPR2750] (ab108961) at 1/5000 dilution

Lane 1:

Active human PKC beta 1 full length protein

Lane 2:

Active human PKC beta 2 full length protein

Lane 3:

Active human PKC gamma full length protein

Lane 3:

Active human PKC delta full length protein

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

• WB

Unknown

Western blot - Anti-PKC gamma antibody [EPR2750] (AB108961)

All lanes:

Western blot - Anti-PKC gamma antibody [EPR2750] (ab108961) at 1/1000 dilution

Lane 1:

Human fetal brain cell lysate at 10 µg

Lane 2:

Human fetal cerebellum cell lysate at 10 µg

Predicted band size: 78 kDa

false