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Rabbit Recombinant Monoclonal KPCG antibody. Carrier free. Suitable for WB, IHC-Fr and reacts with Human, Rat, Mouse samples.

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Images

Western blot - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (AB247722), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (AB247722), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (AB247722), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (AB247722), expandable thumbnail
  • Western blot - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (AB247722), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PIHC-FrICC/IF
Human
Not recommended
Not recommended
Tested
Not recommended
Expected
Not recommended
Mouse
Not recommended
Not recommended
Tested
Not recommended
Tested
Not recommended
Rat
Not recommended
Not recommended
Tested
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Rat, Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Target data

Function

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May be involved in desensitization of mu-type opioid receptor-mediated G-protein activation in the spinal cord, and may be critical for the development and/or maintenance of morphine-induced reinforcing effects in the limbic forebrain. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43 (GJA1/CX43), resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress (By similarity). Phosphorylates p53/TP53 and promotes p53/TP53-dependent apoptosis in response to DNA damage. Involved in the phase resetting of the cerebral cortex circadian clock during temporally restricted feeding. Stabilizes the core clock component BMAL1 by interfering with its ubiquitination, thus suppressing its degradation, resulting in phase resetting of the cerebral cortex clock (By similarity). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed:36040231).

Alternative names

PKCG, PRKCG, Protein kinase C gamma type, PKC-gamma

Recommended products

Rabbit Recombinant Monoclonal KPCG antibody. Carrier free. Suitable for WB, IHC-Fr and reacts with Human, Rat, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR2750
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247722 is the carrier-free version of Anti-PKC gamma antibody [EPR2750] ab108961.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Protein kinase C gamma (PKC gamma) also known as PKCγ is a member of the protein kinase C (PKC) family. This protein has a molecular weight of approximately 78 kDa. Expressed mainly in the central nervous system PKC gamma participates in various signaling pathways. It exists as a serine/threonine kinase and plays a fundamental role in modulating cellular functions in neurons.

Biological function summary

PKC gamma acts in the regulation of neuronal signaling processes and is a component of the synaptic transmission machinery. It associates with other proteins to form complex structures that influence physiological processes. PKC gamma contributes significantly to the functions of the central nervous system by modulating synaptic plasticity which is essential for learning and memory.

Pathways

PKC gamma participates in the MAPK and PI3K signaling pathways which are critical in cell growth and survival. The protein interacts with other PKC isozymes and signaling molecules within these pathways playing a role in phosphorylating specific target proteins. These interactions facilitate the regulation of cell cycle progression and apoptosis indicating its importance in cellular homeostasis.

Associated diseases and disorders

Abnormal PKC gamma activity associates with spinocerebellar ataxia type 14 and addiction-related behavior. Spinocerebellar ataxia results from mutations affecting PKC gamma which disrupts its normal function and leads to neurodegeneration. Additionally PKC gamma’s interaction with other proteins such as GABAA receptors in addiction highlights potential therapeutic targets for treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (ab247722), expandable thumbnail

    Western blot - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (ab247722)

    This data was developed using Anti-PKC gamma antibody [EPR2750] ab108961, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-PKC gamma antibody [EPR2750] (Anti-PKC gamma antibody [EPR2750] ab108961) at 1/1000 dilution

    Lane 1: Human fetal brain cell lysate at 10 µg

    Lane 2: Human fetal cerebellum cell lysate at 10 µg

    Predicted band size: 78 kDa

  • Immunohistochemistry (Frozen sections) - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (ab247722), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (ab247722)

    This data was developed using Anti-PKC gamma antibody [EPR2750] ab108961, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh frozen) tissue labeling PKC gamma with Anti-PKC gamma antibody [EPR2750] ab108961 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).

    Negative control: Confocal image showing no staining on mouse kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-PKC gamma antibody [EPR2750] ab108961 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ?g/ml) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (ab247722), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (ab247722)

    This data was developed using Anti-PKC gamma antibody [EPR2750] ab108961, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh frozen) tissue labeling PKC gamma with Anti-PKC gamma antibody [EPR2750] ab108961 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing positive staining on rat cerebellum. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-PKC gamma antibody [EPR2750] ab108961 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ?g/ml) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (ab247722), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (ab247722)

    This data was developed using Anti-PKC gamma antibody [EPR2750] ab108961, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney (fresh frozen) tissue labeling PKC gamma with Anti-PKC gamma antibody [EPR2750] ab108961 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).

    Negative control: Confocal image showing no staining on rat kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-PKC gamma antibody [EPR2750] ab108961 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ?g/ml) dilution.

  • Western blot - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (ab247722), expandable thumbnail

    Western blot - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (ab247722)

    This data was developed using Anti-PKC gamma antibody [EPR2750] ab108961, the same antibody clone in a different buffer formulation.

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST. In Western blot, anti-GST antibody (Anti-GST antibody [EPR4236] ab111947) loading control staining at 1/1000 dilution. This antibody does not cross-react with human PKC beta 1, human PKC beta 2 and human PKC delta proteins.

    All lanes: Western blot - Anti-PKC gamma antibody [EPR2750] (Anti-PKC gamma antibody [EPR2750] ab108961) at 1/5000 dilution

    Lane 1: Active human PKC beta 1 full length protein

    Lane 2: Active human PKC beta 2 full length protein

    Lane 3: Active human PKC gamma full length protein

    Lane 3: Active human PKC delta full length protein

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Western blot - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (ab247722), expandable thumbnail

    Western blot - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (ab247722)

    This data was developed using Anti-PKC gamma antibody [EPR2750] ab108961, the same antibody clone in a different buffer formulation.

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST. Negative control: Human kidney, mouse kidney, mouse skin, rat skin, rat skeletal muscle. Samples are non-boiled as boiling may cause protein aggregation. In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PKC gamma antibody [EPR2750] (Anti-PKC gamma antibody [EPR2750] ab108961) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 40 µg

    Lane 2: Human kidney tissue lysate at 40 µg

    Lane 3: Mouse brain tissue lysate at 40 µg

    Lane 4: Mouse cerebellum tissue lysate at 40 µg

    Lane 5: Mouse kidney tissue lysate at 40 µg

    Lane 6: Mouse skin tissue lysate at 40 µg

    Lane 7: Rat brain tissue lysate at 40 µg

    Lane 8: Rat skin tissue lysate at 40 µg

    Lane 9: Rat skeletal muscle tissue lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Exposure time: 6s

  • Immunohistochemistry (Frozen sections) - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (ab247722), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-PKC gamma antibody [EPR2750] - BSA and Azide free (ab247722)

    This data was developed using Anti-PKC gamma antibody [EPR2750] ab108961, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh frozen) tissue labeling PKC gamma with Anti-PKC gamma antibody [EPR2750] ab108961 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing positive staining on mouse cerebellum. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-PKC gamma antibody [EPR2750] ab108961 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ?g/ml) dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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