Rabbit Polyclonal PKC iota phospho T555 + T563 antibody. Suitable for Flow Cyt, WB and reacts with Mouse, Human samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Human PRKCI phospho T555 + T563.
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- Frontiers in oncology 13:1051516202314-3-3 and Smad2/3 are crucial mediators of atypical-PKCs: Implications for neuroblastoma progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Breedy,W S Ratnayake,L Lajmi,R Hill,M Acevedo-DuncanPubMed 36776326
- Cellular signalling 77:1098192020The interruption of atypical PKC signaling and Temozolomide combination therapy against glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAvijit Dey,S M Anisul Islam,Rekha Patel,Mildred Acevedo-DuncanPubMed 33147518
- International journal of oncology 56:1373-13862020Simultaneous inhibition of atypical protein kinase‑C and mTOR impedes bladder cancer cell progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRekha Patel et. al.PubMed 32236625
- Protoplasma 257:1149-11632020Towards understanding leydigioma: do G protein-coupled estrogen receptor and peroxisome proliferator-activated receptor regulate lipid metabolism and steroidogenesis in Leydig cell tumors?Applications:Unspecified applicationReactive species:Unspecified reactive speciesM Kotula-Balak et. al.PubMed 32180008
- Translational lung cancer research 8:667-6732019Targeting PKCι-PAK1 in EGFR-mutation positive non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasaoki Ito,Carles Codony-Servat,Niki Karachaliou,Rafael RosellPubMed 31737502
- BMC cancer 19:8042019Clinical significance of atypical protein kinase C (PKCι and PKCζ) and its relationship with yes-associated protein in lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyung-Hee Kim,Chaeuk Chung,Jin-Man Kim,Dahye Lee,Sang Yeon Cho,Tae Hee Lee,Hyun Jin Cho,Min-Kyung YeoPubMed 31412817
- The Journal of neuroscience : the official journal 39:5773-57932019Protein Kinase C Lambda Mediates Acid-Sensing Ion Channel 1a-Dependent Cortical Synaptic Plasticity and Pain Hypersensitivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHu-Song Li et. al.PubMed 31101759
- Cell adhesion & migration 13:106-1202018The modulation of actin dynamics via atypical Protein Kinase-C activated Cofilin regulates metastasis of colorectal cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS M Anisul Islam,Rekha Patel,Raja Reddy Bommareddy,Khandker Mohammad Khalid,Mildred Acevedo-DuncanPubMed 30417717
- Journal of physiology and pharmacology : an offici 69:2018Regulation of steroidogenic function of mouse Leydig cells: G-coupled membrane estrogen receptor and peroxisome proliferator-activated receptor partnership.Applications:Unspecified applicationReactive species:Unspecified reactive speciesE Gorowska-Wojtowicz et. al.PubMed 30149370
- Cellular & molecular biology letters 23:272018Measuring the lactate-to-creatine ratio via H NMR spectroscopy can be used to noninvasively evaluate apoptosis in glioma cells after X-ray irradiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongxia Li,Yi Cui,Fuyan Li,Wenqi Shi,Wenjing Gao,Xiao Wang,Qingshi ZengPubMed 29946338
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PRKCI phospho T555 + T563. The exact immunogen used to generate this antibody is proprietary information. Database link P41743
Reactivity data
Select an application| Flow Cyt | WB | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Expected | Predicted |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI(3)K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non-small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF), acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B and neuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR. This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial cell differentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cell polarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activated by saturated fatty acids and mediates lipid-induced apoptosis. Involved in early synaptic long term potentiation phase in CA1 hippocampal cells and short term memory formation (By similarity).
Alternative names
DXS1179E, PRKCI, Protein kinase C iota type, Atypical protein kinase C-lambda/iota, nPKC-iota, PRKC-lambda/iota, aPKC-lambda/iota
Recommended products
Rabbit Polyclonal PKC iota phospho T555 + T563 antibody. Suitable for Flow Cyt, WB and reacts with Mouse, Human samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Human PRKCI phospho T555 + T563.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PRKCI phospho T555 + T563. The exact immunogen used to generate this antibody is proprietary information. Database link P41743
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody reacts with PKC lambda immunoprecipitates, indicating cross-reactivity for PKC lambda [pT563]. PKC zeta [pT560] (83% homologous) has been shown to cross-react by peptide competition. Peptide competition also suggests that this antibody may partially cross-react with PKC beta 1 [pS642] (58% homologous) and PKC nu [pT655] (42% homologous).
- Concentration
- Purification notes
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKC iota. The final product is generated by affinity chromatography using a PKC iota-derived peptide that is phosphorylated at threonine 555.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Protein Kinase C (PKC) iota also known by its gene symbol PRKCI is an atypical member of the PKC family. It has a molecular mass of approximately 74 kDa. PKC iota does not respond to calcium and is insensitive to diacylglycerol and phorbol esters which differentiates it from other classical PKC family members. The protein initiates cell survival and proliferation pathways and is primarily expressed in brain heart liver and skeletal muscle tissues indicating its extensive role across various biological functions.
- Biological function summary
PKC iota acts as an important player in regulating cell polarity and protecting cells from stress-induced apoptosis. It forms part of the Par6-Par3-PKC iota complex which is important for maintaining cell polarity and directional migration. This complex is essential for neural tube closure during embryonic development and in establishing cell polarity across diverse cell types. PKC iota also contributes to the regulation of microtubule organization reinforcing its vital role in maintaining cellular architecture.
- Pathways
PKC iota is important in the NF-kB signaling pathway and the PI3K/AKT pathway. In the NF-kB pathway PKC iota activates IkB kinase leading to NF-kB transcriptional activity which promotes cell survival. In the PI3K/AKT pathway PKC iota contributes to cell growth and survival working closely with AKT proteins. These pathways highlight its involvement in processes like proliferation survival and cellular response to external stimuli.
- Associated diseases and disorders
PKC iota has a significant connection to cancer and neurological disorders. Its overexpression has been observed in several cancers such as lung and prostate cancer where it is often associated with poor prognosis. PKC iota’s role in the maintenance of cell polarity and survival links it to malignant cell behavior. Additionally in neurological disorders like Alzheimer’s disease alterations in PKC iota activity have been related to synaptic dysfunctions. Connections to proteins such as AKT and NF-kB in these conditions highlight its potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-PKC iota (phospho T555 + T563) antibody (ab5813)
Peptide Competition and Phosphatase Treatment: Lysates prepared from Jurkat cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% low-fat milk-TBST buffer for one hour at room temperature, and incubated with ab5813 antibody for two hours at room temperature in a 3% low-fat milk-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the phosphopeptide corresponding to PKC iota [pT555] blocks the antibody signal. The peptide corresponding to PKC zeta [pT560] blocks the antibody signal and the pepti
All lanes: Western blot - Anti-PKC iota (phospho T555 + T563) antibody (ab5813)
Predicted band size: 68 kDa
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