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AB203188

Anti-PKC nu/PRKD3 antibody

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(1 Publication)

Rabbit Polyclonal PKC nu/PRKD3 antibody. Suitable for IHC-P and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PRKD3 aa 350-450 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

EPK2, PRKCN, PRKD3, Serine/threonine-protein kinase D3, Protein kinase C nu type, Protein kinase EPK2, nPKC-nu

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC nu/PRKD3 antibody (AB203188)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC nu/PRKD3 antibody (AB203188)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human colon carcinoma tissue, labeling PKC nu/PRKD3 using ab203188 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC nu/PRKD3 antibody (AB203188)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC nu/PRKD3 antibody (AB203188)

Immunohistochemical analysis of formalin-fixed paraffin-embedded rat kidney tissue, labeling PKC nu/PRKD3 using ab203188 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human PRKD3 aa 350-450 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O94806

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Cell Lines & Lysates

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Cell Lines & Lysates

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Use at 1/50 - 1/200 with fluorescent detection methods.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Use at 1/50 - 1/200 with fluorescent detection methods.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein kinase C nu also known as PRKD3 is an important serine/threonine kinase located in the protein kinase D family. This protein has a molecular mass of approximately 102 kDa. PRKD3 is mainly expressed in human tissues like the brain heart and lungs. It plays a role in cellular processes by phosphorylating various substrates. The protein can localize to different cellular compartments allowing it to conduct multiple signaling roles effectively.
Biological function summary

PRKD3 influences cellular responses to environmental stress and regulates gene expression. It can promote cell proliferation and survival by being part of a complex signaling network. This protein modulates transcription factors and cellular mechanisms to maintain homeostasis under changing conditions. PRKD3 has interactions with other kinases within its family helping to fine-tune signal transduction processes.

Pathways

PKC nu/PRKD3 integrates into the MAPK signaling cascades essential for proliferative and stress-responses. It also interacts within the NF-kB pathway which regulates immune responses and inflammation. These pathways involve various other proteins like ERKs and IκB kinase positioning PRKD3 as a regulatory node in key cellular activities. This interconnection emphasizes the protein's versatile role in cellular adaptation and signaling.

PRKD3 shows associations with cancer and cardiovascular diseases. The protein's influence over cell growth and apoptosis links it to oncogenic processes making it relevant in cancer research. Additionally its role in heart tissue suggests connections with cardiovascular pathologies. PRKD3 associates with proteins like Bcl-2 in cancer and influences pathways related to heart functions highlighting its relevance in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress (By similarity).
See full target information PRKD3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Metabolism: clinical and experimental 152:155772 PubMed38176644

2024

GDF15 activates AMPK and inhibits gluconeogenesis and fibrosis in the liver by attenuating the TGF-β1/SMAD3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Javier Jurado-Aguilar,Emma Barroso,Maribel Bernard,Meijian Zhang,Mona Peyman,Patricia Rada,Ángela M Valverde,Walter Wahli,Xavier Palomer,Manuel Vázquez-Carrera
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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