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AB252982

Anti-PKC nu/PRKD3 antibody

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(3 Publications)

Rabbit Polyclonal PKC nu/PRKD3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PRKD3 aa 1-150.

View Alternative Names

EPK2, PRKCN, PRKD3, Serine/threonine-protein kinase D3, Protein kinase C nu type, Protein kinase EPK2, nPKC-nu

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PKC nu/PRKD3 antibody (AB252982)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PKC nu/PRKD3 antibody (AB252982)

PFA-fixed, Triton X-100 permeabilized U-2OS (Human brain glioma cell line) cells stained for PKC nu/PRKD3 (green) using ab252982 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC nu/PRKD3 antibody (AB252982)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC nu/PRKD3 antibody (AB252982)

Paraffin-embedded human duodenum tissue stained for PKC nu/PRKD3 using ab252982 at 1/20 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PRKD3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O94806

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein kinase C nu also known as PRKD3 is an important serine/threonine kinase located in the protein kinase D family. This protein has a molecular mass of approximately 102 kDa. PRKD3 is mainly expressed in human tissues like the brain heart and lungs. It plays a role in cellular processes by phosphorylating various substrates. The protein can localize to different cellular compartments allowing it to conduct multiple signaling roles effectively.
Biological function summary

PRKD3 influences cellular responses to environmental stress and regulates gene expression. It can promote cell proliferation and survival by being part of a complex signaling network. This protein modulates transcription factors and cellular mechanisms to maintain homeostasis under changing conditions. PRKD3 has interactions with other kinases within its family helping to fine-tune signal transduction processes.

Pathways

PKC nu/PRKD3 integrates into the MAPK signaling cascades essential for proliferative and stress-responses. It also interacts within the NF-kB pathway which regulates immune responses and inflammation. These pathways involve various other proteins like ERKs and IκB kinase positioning PRKD3 as a regulatory node in key cellular activities. This interconnection emphasizes the protein's versatile role in cellular adaptation and signaling.

PRKD3 shows associations with cancer and cardiovascular diseases. The protein's influence over cell growth and apoptosis links it to oncogenic processes making it relevant in cancer research. Additionally its role in heart tissue suggests connections with cardiovascular pathologies. PRKD3 associates with proteins like Bcl-2 in cancer and influences pathways related to heart functions highlighting its relevance in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress (By similarity).
See full target information PRKD3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 10:836041 PubMed35281088

2022

iTRAQ-Based Proteome Profiling of Differentially Expressed Proteins in Insulin-Resistant Human Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yan,Bei Xie,Ye Tian,Li Huang,Shuli Zou,Zhiheng Peng,Zhuan Liu,Linjing Li

International journal of oral science 13:8 PubMed33692335

2021

PKD3 promotes metastasis and growth of oral squamous cell carcinoma through positive feedback regulation with PD-L1 and activation of ERK-STAT1/3-EMT signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Bomiao Cui,Jiao Chen,Min Luo,Yiying Liu,Hongli Chen,Die Lü,Liwei Wang,Yingzhu Kang,Yun Feng,Libin Huang,Ping Zhang

International journal of oncology 56:909-920 PubMed32319563

2020

Protein kinase D3 regulates the expression of the immunosuppressive protein, PD‑L1, through STAT1/STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Bomiao Cui,Jiao Chen,Min Luo,Liwei Wang,Hongli Chen,Yingzhu Kang,Jingnan Wang,Xuedong Zhou,Yun Feng,Ping Zhang
View all publications
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