AB308385

Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278]

Recombinant
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Rabbit Recombinant Monoclonal KPCZ phospho T410 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat, Recombinant fragment, Drosophila melanogaster samples. Immunogen corresponding to Synthetic Peptide within Human PRKCZ phospho T410.

View Alternative Names

PKC2, PRKCZ, Protein kinase C zeta type, nPKC-zeta

5 Images

Supplier Data

Western blot - Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] (AB308385)

Untreated (–) and treated (+) HeLa whole cell lysates (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1278] (ab308385) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] (ab308385) at 1/1000 dilution

Lane 1:

Untreated (–) HeLa whole cell lysates at 30 µg

Lane 2:

Treated (+) HeLa whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 67.1 kDa

false

Supplier Data

Western blot - Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] (AB308385)

Untreated (–) and treated (+) NIH-3T3 whole cell lysate (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1278] (ab308385) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

All lanes:

Western blot - Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] (ab308385) at 1/1000 dilution

Lane 1:

Untreated (–) NIH-3T3 whole cell lysate at 30 µg

Lane 2:

Treated (+) NIH-3T3 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 67.1 kDa

false

Supplier Data

Western blot - Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] (AB308385)

Untreated (–) and treated (+) PC-12 whole cell lysates (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1278] (ab308385) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] (ab308385) at 1/1000 dilution

Lane 1:

Untreated (–) PC-12 whole cell lysates at 30 µg

Lane 2:

Treated (+) PC-12 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 67.1 kDa

true

Supplier Data

Western blot - Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] (AB308385)

PKC proteins were separated by 10% SDS-PAGE, and the membrane was blotted with PKC (pan) (zeta phospho Thr410) antibody [HL1278] (ab308385) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] (ab308385) at 1/1000 dilution

Lane 1:

Alpha PKC protein at 10 ng

Lane 2:

beta1 PKC protein at 10 ng

Lane 3:

beta2 PKC protein at 10 ng

Lane 4:

gamma PKC protein at 10 ng

Lane 5:

epsilon PKC protein at 10 ng

Lane 6:

zeta PKC protein at 10 ng

Lane 7:

eta PKC protein at 10 ng

Lane 8:

omega PKC protein at 10 ng

Lane 9:

delta PKC protein at 200 ng

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 67.1 kDa

false

Supplier Data

Western blot - Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] (AB308385)

All lanes:

Western blot - Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] (ab308385) at 1/1000 dilution

All lanes:

Drosophila brain tissue at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1278

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat, Drosophila melanogaster

Applications

applications

Immunogen

Synthetic Peptide within Human PRKCZ phospho T410. The exact immunogen used to generate this antibody is proprietary information.

Q05513

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Drosophila melanogaster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PKC zeta also known as Protein Kinase C zeta is a member of the atypical protein kinase C family. This enzyme plays an important role in various cellular processes. PKC zeta has a molecular mass of about 55 kDa and is expressed in many tissues including brain and immune cells. Unlike conventional PKC enzymes PKC zeta does not respond to calcium or diacylglycerol which influences its unique regulatory roles.

Biological function summary

PKC zeta participates in regulating cell proliferation survival and differentiation. It often functions as part of signaling complexes that include other proteins like PDK1 and is involved in transducing signals from cell surface receptors to the nucleus. Its activity influences cellular responses to hormones growth factors and cytokines contributing to diverse cellular events.

Pathways

PKC zeta plays a critical role in the NF-kappaB and PI3K/Akt signaling pathways. In the NF-kappaB pathway PKC zeta helps in the activation of this transcription factor affecting genes related to inflammation and immune response. In the PI3K/Akt pathway it is involved in cell growth and survival interacting with kinases such as Akt and PDK1 which further modulate downstream effectors.

PKC zeta has associations with cancer and insulin resistance-related conditions. In cancer dysregulation of PKC zeta can lead to unchecked cellular proliferation due to its involvement in survival pathways and it often interacts with oncogenic proteins like Akt. In insulin resistance PKC zeta misregulation impacts glucose metabolism highlighting its role in diabetes-related pathways alongside insulin receptor substrates.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Phosphorylates VAMP2 in vitro (PubMed : 17313651). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed : 36040231).. Isoform 2. Involved in late synaptic long term potention phase in CA1 hippocampal cells and long term memory maintenance.

See full target information PRKCZ phospho T410

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278]

Western blot - Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] (AB308385)

Western blot - Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] (AB308385)

Western blot - Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] (AB308385)

Western blot - Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] (AB308385)

Western blot - Anti-PKC (pan) (zeta phospho Thr410) antibody [HL1278] (AB308385)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise