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Rabbit Recombinant Monoclonal KPCG phospho T514 antibody. Suitable for IHC-P, IP, WB, I-ELISA, Dot and reacts with Mouse, Rat, Human, Synthetic peptide - Human, Synthetic peptide samples. Cited in 14 publications.

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Images

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539), expandable thumbnail
  • Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539), expandable thumbnail
  • Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539), expandable thumbnail
  • Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539), expandable thumbnail
  • Immunoprecipitation - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBI-ELISADot
Human
Tested
Expected
Tested
Expected
Expected
Mouse
Tested
Expected
Tested
Expected
Expected
Rat
Tested
Tested
Tested
Expected
Expected
Synthetic peptide
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Synthetic peptide - Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/300

Notes

For unpurified use at 1/100 - 1/250.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/300

Notes

For unpurified use at 1/100 - 1/250.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/300

Notes

For unpurified use at 1/100 - 1/250.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Synthetic peptide - Human, Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/20

Notes

For unpurified use at 1/50.

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human, Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000.00000 - 1/10000.00000

Notes

-

Species

Rat

Dilution info

1/1000.00000 - 1/10000.00000

Notes

-

Species

Human

Dilution info

1/1000.00000 - 1/10000.00000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human, Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide - Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May be involved in desensitization of mu-type opioid receptor-mediated G-protein activation in the spinal cord, and may be critical for the development and/or maintenance of morphine-induced reinforcing effects in the limbic forebrain. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43 (GJA1/CX43), resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress (By similarity). Phosphorylates p53/TP53 and promotes p53/TP53-dependent apoptosis in response to DNA damage. Involved in the phase resetting of the cerebral cortex circadian clock during temporally restricted feeding. Stabilizes the core clock component BMAL1 by interfering with its ubiquitination, thus suppressing its degradation, resulting in phase resetting of the cerebral cortex clock (By similarity). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed:36040231).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal KPCG phospho T514 antibody. Suitable for IHC-P, IP, WB, I-ELISA, Dot and reacts with Mouse, Rat, Human, Synthetic peptide - Human, Synthetic peptide samples. Cited in 14 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP2730Y

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Protein kinase C (PKC) comprises a family of serine/threonine kinases with several isoforms such as PKC alpha beta and gamma among others. PKC enzymes play key roles in intracellular signaling particularly in mediating responses to growth factors hormones and other signals. PKC exhibits a molecular weight generally ranging around 77 to 97 kDa depending on the isoform. These enzymes show expression in many tissues but have high concentrations in the brain heart and lung. PKC activation often involves translocation to cellular membranes which is important for their signaling roles.

Biological function summary

PKC influences various cellular processes such as cell proliferation differentiation apoptosis and immune responses. It acts within larger protein complexes serving as a modulator of cellular functions. The activation of PKC leads to its association with different scaffolding proteins impacting a wide array of cell activities. PKC notably affects the function of other kinases and transcription factors demonstrating its integral role in managing cellular behavior and homeostasis.

Pathways

PKC participates in the phosphoinositide signaling pathway and the mitogen-activated protein kinase (MAPK) pathway. In these contexts PKC activation impacts proteins like Ras and Raf kinases which play roles in cellular growth and differentiation. By modulating these pathways PKC becomes an important component in signal transduction cascades influencing cell fate decisions. Understanding the interaction between PKC and these pathways helps in elucidating its role in maintaining normal cellular activities and responses to external stimuli.

Associated diseases and disorders

PKC is linked to cancer and cardiovascular diseases. In cancer dysregulation of PKC activity often contributes to tumor growth and progression by altering pathways that control cell division and survival. PKC abnormally interacts with proteins like Bcl-2 promoting cell survival. In cardiovascular diseases changes in PKC signaling can impact heart function contributing to conditions like heart failure through interactions with proteins such as troponin. Research on PKC and these diseases can provide insights into developing targeted therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539), expandable thumbnail

    Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539)

    Blocking and diluting buffer : 5% NFDM/TBST

    All lanes: Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539) at 1/2000 dilution

    Lane 1: Rat brain lysates at 15 µg

    Lane 2: Rat brain lysates and then the membrane was incubated with phosphatase. at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 77 kDa

  • Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539), expandable thumbnail

    Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539)

    Blocking and diluting buffer : 5% NFDM/TBST

    All lanes: Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539) at 1/2000 dilution

    Lane 1: Mouse cerebellum lysates at 15 µg

    Lane 2: Mouse cerebellum lysates and then the membrane was incubated with phosphatase. at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 77 kDa

  • Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539), expandable thumbnail

    Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539) at 1/5000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) treated with Phorbol-12-myristate-13-acetate whole cell lysates at 15 µg

    Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) treated with Phorbol-12-myristate-13-acetate whole cell lysates. The membrane was then incubated with phosphatase. at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 77 kDa

    Observed band size: 80 kDa

    Exposure time: 3min

    This data was developed using ab109539, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539), expandable thumbnail

    Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539)

    All lanes: Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539) at 0.02 µg/mL

    All lanes: HeLa cell lysate

    Predicted band size: 77 kDa

    Observed band size: 80 kDa

    Exposure time: 15s

  • Immunoprecipitation - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539), expandable thumbnail

    Immunoprecipitation - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539)

    ab109539 (purified) at 1:20 dilution (2μg) immunoprecipitating PKC in Rat brain lysate.
    Lane 1 (input): Rat brain lysate 10μg
    Lane 2 (+): ab109539 & Rat brain lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab109539 in Rat brain lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539)

    Predicted band size: 77 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebellum tissue sections labeling PKC with Purified ab109539 at 1:300 dilution (1.14 μg/ml). Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebellum tissue sections labeling PKC with Purified ab109539 at 1:300 dilution (1.14 μg/ml). Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebral cortex tissue sections labeling PKC with Purified ab109539 at 1:300 dilution (1.14 μg/ml). Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539)

    Unpurified ab109539 at 1/100 dilution staining PKC (phospho T514) in paraffin-embedded Human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Indirect ELISA - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539), expandable thumbnail

    Indirect ELISA - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539)

    Various phosphorylated PKC peptides at 1000 ng/ml stained for PKC (phospho T514) using ab109539 at 0-1000 ng/ml in ELISA. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) was used as a secondary antibody at a concentration of 1/2500.


    Antigen:

    Human PKC gamma (pT514)

    Human PKC alpha (pT497)

    Human PKC beta (pT500)

    Human PKC delta (pT507)

    Human PKC eta (pT513)

    Human PKC epsilon (pT566)

    Human PKC theta (pT538)

    Human PKC gamma non-phospho

  • Dot Blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539), expandable thumbnail

    Dot Blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539)

    Various phosphorylated PKC peptides stained for PKC (phospho T514) using ab109539 at 0.543 μg/ml in Dot blot. Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at a dilution of 1/100,000. Exposure time: 180 seconds.

    Blocking and dilution buffer: 5% NFDM/TBST.


    Lane 1: Human PKC gamma (pT514) peptide

    Lane 2: Human PKC alpha (pT497) peptide

    Lane 3: Human PKC gamma non-phospho peptide

    Lane 4: Human PKC beta (pT500) peptide

    Lane 5: Human PKC delta (pT507) peptide

    Lane 6: Human PKC eta (pT513) peptide

    Lane 7: Human PKC epsilon (pT566) peptide

    Lane 8: Human PKC theta (pT538) peptide

    ab109539 could detect PKC gamma (pT514), PKC alpha (pT497), PKC beta (pT500), PKC delta (pT507), PKC eta (pT513), PKC epsilon (pT566) and PKC theta (pT538).

    All lanes: Dot Blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539)

    Lane 1: Human PKC gamma (pT514) peptide

    Lane 2: Human PKC alpha (pT497) peptide

    Lane 3: Human PKC gamma non-phospho peptide

    Lane 4: Human PKC beta (pT500) peptide

    Lane 5: Human PKC delta (pT507) peptide

    Lane 6: Human PKC eta (pT513) peptide

    Lane 7: Human PKC epsilon (pT566) peptide

    Lane 8: Human PKC theta (pT538) peptide

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539), expandable thumbnail

    Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539)

    Blocking and dilution buffer: 5% NFDM/TBST.

    ab109539 could detect PKC gamma (pT514), PKC alpha (pT497), PKC beta (pT500), PKC delta (pT507), PKC eta (pT513), PKC epsilon (pT566) and PKC theta (pT538).




    Active human PKC alpha full length protein (Catalog#Recombinant human PKC alpha protein (Active) ab55672) contains aa1-672 with GST-tag;

    Active human PKC beta 1 full length protein (Catalog#Recombinant human PKC beta 1 protein ab60840) contains aa1-671 with GST-tag;

    Active human PKC beta 2 full length protein (Catalog#Recombinant human PKC beta 2 protein ab60841) contains aa1-673 with GST-tag;

    Active human PKC delta full length protein (Catalog#Recombinant human PKC delta protein ab60844) contains aa1-676 with GST-tag;

    Active human PKC eta full length protein (Catalog#Recombinant human PKC eta protein ab60849) contains aa1-683 with GST-tag;

    Active human PKC epsilon full length protein (Catalog#Recombinant human PKC epsilon protein ab60847) contains aa1-737 with GST-tag;

    Active human PKC theta full length protein (Catalog#Recombinant human PKC theta/PRKCQ protein ab56641) contains aa1-706 with GST-tag;

    Active human PKC mu full length protein (Catalog#Recombinant human PKC mu/PKD protein ab60873) contains aa1-912 with GST-tag;

    Active human PKC gamma full length protein (Catalog#Recombinant human PKC gamma protein ab60842) contains aa1-697 with GST-tag.

    All lanes: Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539) at 1/1000 dilution

    Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (Recombinant human PKC alpha protein (Active) ab55672) at 0.5 µg

    Lane 2: Western blot - Recombinant human PKC beta 1 protein (Recombinant human PKC beta 1 protein ab60840) at 0.2 µg

    Lane 3: Active human PKC beta 2 full length protein at 0.2 µg

    Lane 4: Western blot - Recombinant human PKC delta protein (Recombinant human PKC delta protein ab60844) at 0.05 µg

    Lane 5: Western blot - Recombinant human PKC eta protein (Recombinant human PKC eta protein ab60849) at 0.5 µg

    Lane 6: Western blot - Recombinant human PKC epsilon protein (Recombinant human PKC epsilon protein ab60847) at 1 µg

    Lane 7: Western blot - Recombinant human PKC theta/PRKCQ protein (Recombinant human PKC theta/PRKCQ protein ab56641) at 0.2 µg

    Lane 8: Western blot - Recombinant human PKC mu/PKD protein (Recombinant human PKC mu/PKD protein ab60873) at 1 µg

    Lane 9: Western blot - Recombinant human PKC gamma protein (Recombinant human PKC gamma protein ab60842) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 77 kDa

    Observed band size: 80 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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