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AB247898

Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free

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(2 Publications)

Rabbit Recombinant Monoclonal KPCG phospho T514 antibody. Carrier free. Suitable for IHC-P, IP, WB, I-ELISA, Dot and reacts with Rat, Mouse, Human, Synthetic peptide - Human, Synthetic peptide samples. Cited in 2 publications.

View Alternative Names

PKCG, PRKCG, Protein kinase C gamma type, PKC-gamma

12 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)

This data was developed using ab109539, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebral cortex tissue sections labeling PKC with Purified ab109539 at 1 : 300 dilution (1.14 μg/ml). Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)

This data was developed using ab109539, the same antibody clone in a different buffer formulation.

Unpurified ab109539 at 1/100 dilution staining PKC (phospho T514) in paraffin-embedded Human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Indirect ELISA - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)
  • I-ELISA

Lab

Indirect ELISA - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109539)

Various phosphorylated PKC peptides at 1000 ng/ml stained for PKC (phospho T514) using ab109539 at 0-1000 ng/ml in ELISA. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) was used as a secondary antibody at a concentration of 1/2500.

Antigen :
Human PKC gamma (pT514)
Human PKC alpha (pT497)
Human PKC beta (pT500)
Human PKC delta (pT507)
Human PKC eta (pT513)
Human PKC epsilon (pT566)
Human PKC theta (pT538)
Human PKC gamma non-phospho

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)

This data was developed using ab109539, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebellum tissue sections labeling PKC with Purified ab109539 at 1 : 300 dilution (1.14 μg/ml). Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)

This data was developed using ab109539, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebellum tissue sections labeling PKC with Purified ab109539 at 1 : 300 dilution (1.14 μg/ml). Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

Immunoprecipitation - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)
  • IP

Unknown

Immunoprecipitation - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)

This data was developed using ab109539, the same antibody clone in a different buffer formulation.

ab109539 (purified) at 1 : 20 dilution (2μg) immunoprecipitating PKC in Rat brain lysate.
Lane 1 (input) : Rat brain lysate 10μg
Lane 2 (+) : ab109539 & Rat brain lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab109539 in Rat brain lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-PKC (phospho T514) antibody [EP2730Y] (<a href='/en-us/products/primary-antibodies/pkc-phospho-t514-antibody-ep2730y-ab109539'>ab109539</a>)

Predicted band size: 77 kDa

false

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)
  • WB

Lab

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)

This data was developed using ab109539, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (<a href='/en-us/products/primary-antibodies/pkc-phospho-t514-antibody-ep2730y-ab109539'>ab109539</a>) at 0.02 µg/mL

Lane 1:

HeLa cell lysate with PKC gamma (pT514) peptide

Lane 2:

HeLa cell lysate with PKC gamma non-phospho peptide

Lane 3:

HeLa cell lysate

Predicted band size: 77 kDa

Observed band size: 80 kDa

false

Exposure time: 15s

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)
  • WB

Lab

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109539).

Blocking and dilution buffer : 5% NFDM/TBST.

ab109539 could detect PKC gamma (pT514), PKC alpha (pT497), PKC beta (pT500), PKC delta (pT507), PKC eta (pT513), PKC epsilon (pT566) and PKC theta (pT538).

Active human PKC alpha full length protein (Catalog#ab55672) contains aa1-672 with GST-tag;
Active human PKC beta 1 full length protein (Catalog#ab60840) contains aa1-671 with GST-tag;
Active human PKC beta 2 full length protein (Catalog#ab60841) contains aa1-673 with GST-tag;
Active human PKC delta full length protein (Catalog#ab60844) contains aa1-676 with GST-tag;
Active human PKC eta full length protein (Catalog#ab60849) contains aa1-683 with GST-tag;
Active human PKC epsilon full length protein (Catalog#ab60847) contains aa1-737 with GST-tag;
Active human PKC theta full length protein (Catalog#ab56641) contains aa1-706 with GST-tag;
Active human PKC mu full length protein (Catalog#ab60873) contains aa1-912 with GST-tag;
Active human PKC gamma full length protein (Catalog#ab60842) contains aa1-697 with GST-tag.

All lanes:

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (<a href='/en-us/products/primary-antibodies/pkc-phospho-t514-antibody-ep2730y-ab109539'>ab109539</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>) at 0.5 µg

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>) at 0.2 µg

Lane 3:

Active human PKC beta 2 full length protein at 0.2 µg

Lane 4:

Western blot - Recombinant human PKC delta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>) at 0.05 µg

Lane 5:

Western blot - Recombinant human PKC eta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>) at 0.5 µg

Lane 6:

Western blot - Recombinant human PKC epsilon protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>) at 1 µg

Lane 7:

Western blot - Recombinant human PKC theta/PRKCQ protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>) at 0.2 µg

Lane 8:

Western blot - Recombinant human PKC mu/PKD protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-mu-pkd-protein-ab60873'>ab60873</a>) at 1 µg

Lane 9:

Western blot - Recombinant human PKC gamma protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 77 kDa

Observed band size: 80 kDa

false

Exposure time: 180s

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)
  • WB

Lab

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)

This data was developed using ab109539, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (<a href='/en-us/products/primary-antibodies/pkc-phospho-t514-antibody-ep2730y-ab109539'>ab109539</a>) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with Phorbol-12-myristate-13-acetate whole cell lysates at 15 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with Phorbol-12-myristate-13-acetate whole cell lysates, the membrane was then incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 77 kDa

Observed band size: 80 kDa

false

Exposure time: 3min

Dot Blot - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)
  • Dot

Lab

Dot Blot - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)

Various phosphorylated PKC peptides stained for PKC (phospho T514) using ab109539 at 0.543 μg/ml in Dot blot. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at a dilution of 1/100,000. Exposure time : 180 seconds.

Blocking and dilution buffer : 5% NFDM/TBST.

Lane 1 : Human PKC gamma (pT514) peptide
Lane 2 : Human PKC alpha (pT497) peptide
Lane 3 : Human PKC gamma non-phospho peptide
Lane 4 : Human PKC beta (pT500) peptide
Lane 5 : Human PKC delta (pT507) peptide
Lane 6 : Human PKC eta (pT513) peptide
Lane 7 : Human PKC epsilon (pT566) peptide
Lane 8 : Human PKC theta (pT538) peptide

ab109539 could detect PKC gamma (pT514), PKC alpha (pT497), PKC beta (pT500), PKC delta (pT507), PKC eta (pT513), PKC epsilon (pT566) and PKC theta (pT538).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109539)

All lanes:

Dot Blot - Anti-PKC (phospho T514) antibody [EP2730Y] (<a href='/en-us/products/primary-antibodies/pkc-phospho-t514-antibody-ep2730y-ab109539'>ab109539</a>)

Lane 1:

Human PKC gamma (pT514) peptide

Lane 2:

Human PKC alpha (pT497) peptide

Lane 3:

Human PKC gamma non-phospho peptide

Lane 4:

Human PKC beta (pT500) peptide

Lane 5:

Human PKC delta (pT507) peptide

Lane 6:

Human PKC eta (pT513) peptide

Lane 7:

Human PKC epsilon (pT566) peptide

Lane 8:

Human PKC theta (pT538) peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)
  • WB

Unknown

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)

This data was developed using ab109539, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (<a href='/en-us/products/primary-antibodies/pkc-phospho-t514-antibody-ep2730y-ab109539'>ab109539</a>) at 1/2000 dilution

Lane 1:

Rat brain lysates at 15 µg

Lane 2:

Rat brain lysates and then the membrane was incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 77 kDa

false

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)
  • WB

Unknown

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] - BSA and Azide free (AB247898)

This data was developed using ab109539, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (<a href='/en-us/products/primary-antibodies/pkc-phospho-t514-antibody-ep2730y-ab109539'>ab109539</a>) at 1/2000 dilution

Lane 1:

Mouse cerebellum lysates at 15 µg

Lane 2:

Mouse cerebellum lysates and then the membrane was incubated with phosphatase. at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 77 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2730Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, Dot, WB, I-ELISA, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

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Primary Antibodies

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Anti-PKC zeta antibody [EP1490(2)]

RabMAb|KO Validated

Western blot - Anti-PKC zeta antibody [EP1490(2)] (AB108970)

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  • 1
Primary Antibodies

AB32376

Anti-PKC alpha antibody [Y124]

20ul selling size|RabMAb|Recombinant|KO Validated

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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Synthetic peptide": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>" }, "Synthetic peptide - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "" } } }
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Product details

ab247898 is the carrier-free version of ab109539.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein kinase C (PKC) comprises a family of serine/threonine kinases with several isoforms such as PKC alpha beta and gamma among others. PKC enzymes play key roles in intracellular signaling particularly in mediating responses to growth factors hormones and other signals. PKC exhibits a molecular weight generally ranging around 77 to 97 kDa depending on the isoform. These enzymes show expression in many tissues but have high concentrations in the brain heart and lung. PKC activation often involves translocation to cellular membranes which is important for their signaling roles.
Biological function summary

PKC influences various cellular processes such as cell proliferation differentiation apoptosis and immune responses. It acts within larger protein complexes serving as a modulator of cellular functions. The activation of PKC leads to its association with different scaffolding proteins impacting a wide array of cell activities. PKC notably affects the function of other kinases and transcription factors demonstrating its integral role in managing cellular behavior and homeostasis.

Pathways

PKC participates in the phosphoinositide signaling pathway and the mitogen-activated protein kinase (MAPK) pathway. In these contexts PKC activation impacts proteins like Ras and Raf kinases which play roles in cellular growth and differentiation. By modulating these pathways PKC becomes an important component in signal transduction cascades influencing cell fate decisions. Understanding the interaction between PKC and these pathways helps in elucidating its role in maintaining normal cellular activities and responses to external stimuli.

PKC is linked to cancer and cardiovascular diseases. In cancer dysregulation of PKC activity often contributes to tumor growth and progression by altering pathways that control cell division and survival. PKC abnormally interacts with proteins like Bcl-2 promoting cell survival. In cardiovascular diseases changes in PKC signaling can impact heart function contributing to conditions like heart failure through interactions with proteins such as troponin. Research on PKC and these diseases can provide insights into developing targeted therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May be involved in desensitization of mu-type opioid receptor-mediated G-protein activation in the spinal cord, and may be critical for the development and/or maintenance of morphine-induced reinforcing effects in the limbic forebrain. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43 (GJA1/CX43), resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress (By similarity). Phosphorylates p53/TP53 and promotes p53/TP53-dependent apoptosis in response to DNA damage. Involved in the phase resetting of the cerebral cortex circadian clock during temporally restricted feeding. Stabilizes the core clock component BMAL1 by interfering with its ubiquitination, thus suppressing its degradation, resulting in phase resetting of the cerebral cortex clock (By similarity). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed : 36040231).
See full target information PRKCG pT514
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Phytotherapy research : PTR 37:4236-4250 PubMed37329155

2023

Anti-inflammatory effect of dictamnine on allergic rhinitis via suppression of the LYN kinase-mediated molecular signaling pathway during mast cell activation.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Liu,Yonghui Zhang,Yuejin Wang,Yihan Huang,Jiapan Gao,Xi Tian,Tianyou Ma,Tao Zhang

Immunity, inflammation and disease 10:e692 PubMed36301023

2022

HDAC4 depletion ameliorates IL-13-triggered inflammatory response and mucus production in nasal epithelial cells via activation of SIRT1/NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hangyu Xu,Lingjun Wang,Huaqun Chen,Hefei Cai
View all publications
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Product promise

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