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Mouse Recombinant Monoclonal PKC theta/PRKCQ antibody. Suitable for WB, IP and reacts with Human, Mouse samples.

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Images

Western blot - Anti-PKC theta/PRKCQ antibody [27/PKC theta] (AB302891), expandable thumbnail
  • Western blot - Anti-PKC theta/PRKCQ antibody [27/PKC theta] (AB302891), expandable thumbnail
  • Western blot - Anti-PKC theta/PRKCQ antibody [27/PKC theta] (AB302891), expandable thumbnail
  • Immunoprecipitation - Anti-PKC theta/PRKCQ antibody [27/PKC theta] (AB302891), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFIP
Human
Tested
Not recommended
Not recommended
Tested
Mouse
Tested
Not recommended
Not recommended
Expected
Rat
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Target data

Function

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that mediates non-redundant functions in T-cell receptor (TCR) signaling, including T-cells activation, proliferation, differentiation and survival, by mediating activation of multiple transcription factors such as NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated T-cells, is required for the activation of NF-kappa-B and JUN, which in turn are essential for IL2 production, and participates in the calcium-dependent NFATC1 and NFATC2 transactivation (PubMed:21964608). Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11 on several serine residues, inducing CARD11 association with lipid rafts and recruitment of the BCL10-MALT1 complex, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May also play an indirect role in activation of the non-canonical NF-kappa-B (NFKB2) pathway. In the signaling pathway leading to JUN activation, acts by phosphorylating the mediator STK39/SPAK and may not act through MAP kinases signaling. Plays a critical role in TCR/CD28-induced NFATC1 and NFATC2 transactivation by participating in the regulation of reduced inositol 1,4,5-trisphosphate generation and intracellular calcium mobilization. After costimulation of T-cells through CD28 can phosphorylate CBLB and is required for the ubiquitination and subsequent degradation of CBLB, which is a prerequisite for the activation of TCR. During T-cells differentiation, plays an important role in the development of T-helper 2 (Th2) cells following immune and inflammatory responses, and, in the development of inflammatory autoimmune diseases, is necessary for the activation of IL17-producing Th17 cells. May play a minor role in Th1 response. Upon TCR stimulation, mediates T-cell protective survival signal by phosphorylating BAD, thus protecting T-cells from BAD-induced apoptosis, and by up-regulating BCL-X(L)/BCL2L1 levels through NF-kappa-B and JUN pathways. In platelets, regulates signal transduction downstream of the ITGA2B, CD36/GP4, F2R/PAR1 and F2RL3/PAR4 receptors, playing a positive role in 'outside-in' signaling and granule secretion signal transduction. May relay signals from the activated ITGA2B receptor by regulating the uncoupling of WASP and WIPF1, thereby permitting the regulation of actin filament nucleation and branching activity of the Arp2/3 complex. May mediate inhibitory effects of free fatty acids on insulin signaling by phosphorylating IRS1, which in turn blocks IRS1 tyrosine phosphorylation and downstream activation of the PI3K/AKT pathway. Phosphorylates MSN (moesin) in the presence of phosphatidylglycerol or phosphatidylinositol. Phosphorylates PDPK1 at 'Ser-504' and 'Ser-532' and negatively regulates its ability to phosphorylate PKB/AKT1. Phosphorylates CCDC88A/GIV and inhibits its guanine nucleotide exchange factor activity (PubMed:23509302). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed:36040231).

Alternative names

PRKCT, PRKCQ, Protein kinase C theta type, nPKC-theta

Recommended products

Mouse Recombinant Monoclonal PKC theta/PRKCQ antibody. Suitable for WB, IP and reacts with Human, Mouse samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
27-PKC theta
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PKC theta also known as PRKCQ is a protein kinase involved in signal transduction. The mass of PKC theta is approximately 85 kDa. It is a member of the protein kinase C (PKC) family which has distinctive roles in cellular processes. PKC theta specifically localizes in T-cells and is an important player in immune system functions. It is expressed predominantly in lymphoid tissues such as the spleen and thymus where T-cell activation and differentiation are significant.

Biological function summary

PKC theta participates in T-cell receptor signaling involved in the formation of immune synapses. It is an essential component of the multiprotein complex that includes molecules like LAT SLP-76 and Lck. PKC theta's activation allows the transmission of activation signals leading to the production of cytokines like IL-2. This function is integral to ensuring an effective immune response and the maintenance of immune homeostasis.

Pathways

PKC theta is central in the NF-kB pathway which transmits signals from T-cell receptors to the nucleus promoting gene transcription. It also plays a role in the AP-1 pathway collaborating with proteins such as CARMA1 and BCL10. These pathways underpin the immune response coordinating T-cell activation proliferation and survival linking PKC theta function to several immunological processes.

Associated diseases and disorders

PKC theta has connections with autoimmune conditions such as rheumatoid arthritis and type 1 diabetes. In these disorders its role in T-cell signaling becomes dysregulated. This can lead to the overactive immune response against the body's tissues. Additionally PKC theta may interact with proteins involved in inflammatory signaling like NF-kB exacerbating inflammation and tissue damage. Understanding these relationships is important for developing therapies targeting immune dysregulation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-PKC theta/PRKCQ antibody [27/PKC theta] (ab302891), expandable thumbnail

    Western blot - Anti-PKC theta/PRKCQ antibody [27/PKC theta] (ab302891)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The blot of lane 4 was developed using a high sensitivity ECL substrate.

    Exposure time: Lane 1-3: 92 seconds; Lane 4: 59 seconds.

    All lanes: Western blot - Anti-PKC theta/PRKCQ antibody [27/PKC theta] (ab302891) at 1/1000 dilution

    Lane 1: Mouse spleen tissue lysate at 20 µg

    Lane 2: Mouse thymus tissue lysate at 20 µg

    Lane 3: K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

    Lane 4: Human cerebellum tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

    Developed using the ECL technique.

    Observed band size: 81 kDa

  • Western blot - Anti-PKC theta/PRKCQ antibody [27/PKC theta] (ab302891), expandable thumbnail

    Western blot - Anti-PKC theta/PRKCQ antibody [27/PKC theta] (ab302891)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 26 seconds.

    All lanes: Western blot - Anti-PKC theta/PRKCQ antibody [27/PKC theta] (ab302891) at 1/1000 dilution

    Lane 1: Active human PKC beta 1 fl length protein 5 ng at 5 ng

    Lane 2: Active human PKC delta fl length protein 5 ng at 5 ng

    Lane 3: Active human PKC theta fl length protein 10 ng at 10 ng

    Lane 4: Active human PKC zeta fl length protein 10 ng at 10 ng

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

    Observed band size: 100 kDa

    Exposure time: 26s

  • Western blot - Anti-PKC theta/PRKCQ antibody [27/PKC theta] (ab302891), expandable thumbnail

    Western blot - Anti-PKC theta/PRKCQ antibody [27/PKC theta] (ab302891)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 37 seconds

    All lanes: Western blot - Anti-PKC theta/PRKCQ antibody [27/PKC theta] (ab302891) at 1/1000 dilution

    Lane 1: Active human PKC alpha fl length protein 60 ng at 60 ng

    Lane 2: Active human PKC beta 2 fl length protein 5 ng at 5 ng

    Lane 3: Active human PKC epsilon fl length protein 50 ng at 50 ng

    Lane 4: Active human PKC theta fl length protein 5 ng at 5 ng

    Lane 5: Active human PKC mu fl length protein 50 ng at 50 EU/ng

    Lane 6: Active human PKC iota fl length protein 10 ng at 10 ng

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

    Observed band size: 100 kDa

    Exposure time: 37s

  • Immunoprecipitation - Anti-PKC theta/PRKCQ antibody [27/PKC theta] (ab302891), expandable thumbnail

    Immunoprecipitation - Anti-PKC theta/PRKCQ antibody [27/PKC theta] (ab302891)

    PKC theta/PRKCQ was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate 10 µg with ab302891 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302891 at 1/1000 dilution. Peroxidase-Conjµated Goat anti-Mouse IgG (H+L) was used at 1/10000 dilution.

    Lane 1: K-562 whole cell lysate 10 µg

    Lane 2: ab302891 IP in K-562 whole cell lysate

    Lane 3: Mouse IgG2a, kappa monoclonal isotype control (Mouse IgG2a, Kappa Monoclonal [MOPC-173] -Isotype Control - ChIP Grade ab18413) instead of ab302891 in K-562 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-PKC theta/PRKCQ antibody [27/PKC theta] (ab302891) at 1/30 dilution

    All lanes: K-562 (human chronic myelogenous leukemia lymphoblast)

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 82 kDa

    Exposure time: 3s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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