AB108970

Anti-PKC zeta antibody [EP1490(2)]

Name: Anti-PKC zeta antibody [EP1490(2)] (ab108970) | Abcam
Brand: Abcam Limited
SKU: AB108970
Price: 630 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
KO Validated
What is this?

(1 Review)

(8 Publications)

Rabbit Monoclonal KPCZ antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 8 publications.

View Alternative Names

PKC2, PRKCZ, Protein kinase C zeta type, nPKC-zeta

3 Images

Lab

Western blot - Anti-PKC zeta antibody [EP1490(2)] (AB108970)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : PKC zeta knockout HAP1 cell lysate (20 μg)
Lane 3 : HT-29 cell lysate (20 μg)
Lane 4 : A431 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab108970 observed at 74 kDa. Red - loading control, ab8226, observed at 42 kDa.
ab108970 was shown to specifically react with PKC zeta when PKC zeta knockout samples were used. Wild-type and PKC zeta knockout samples were subjected to SDS-PAGE. ab108970 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-PKC zeta antibody [EP1490(2)] (ab108970)

Predicted band size: 68 kDa

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Lab

Western blot - Anti-PKC zeta antibody [EP1490(2)] (AB108970)

Lanes 1 - 3 : Merged signal (red and green). Green - ab108970 observed at 74 kDa. Red - loading control, ab8245, observed at 37 kDa.

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab108970 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at a 1 : 10000 dilution for 1hr at room temperature and then imaged.

All lanes:

Western blot - Anti-PKC zeta antibody [EP1490(2)] (ab108970) at 1/1000 dilution

Lane 1:

A431 whole cell lysate at 20 µg

Lane 2:

Mouse lung tissue lysate at 20 µg

Lane 3:

Rat lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 68 kDa

Observed band size: 74 kDa

false

Unknown

Western blot - Anti-PKC zeta antibody [EP1490(2)] (AB108970)

All lanes:

Western blot - Anti-PKC zeta antibody [EP1490(2)] (ab108970) at 1/1000 dilution

Lane 1:

293T lysate at 10 µg

Lane 2:

HT-29 lysate at 10 µg

Lane 3:

Fetal liver lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 68 kDa

Observed band size: 74 kDa

false

Carrier free

Anti-PKC zeta antibody [EP1490(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1490(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PKC zeta also known as Protein Kinase C zeta is a member of the atypical protein kinase C family. This enzyme plays an important role in various cellular processes. PKC zeta has a molecular mass of about 55 kDa and is expressed in many tissues including brain and immune cells. Unlike conventional PKC enzymes PKC zeta does not respond to calcium or diacylglycerol which influences its unique regulatory roles.

Biological function summary

PKC zeta participates in regulating cell proliferation survival and differentiation. It often functions as part of signaling complexes that include other proteins like PDK1 and is involved in transducing signals from cell surface receptors to the nucleus. Its activity influences cellular responses to hormones growth factors and cytokines contributing to diverse cellular events.

Pathways

PKC zeta plays a critical role in the NF-kappaB and PI3K/Akt signaling pathways. In the NF-kappaB pathway PKC zeta helps in the activation of this transcription factor affecting genes related to inflammation and immune response. In the PI3K/Akt pathway it is involved in cell growth and survival interacting with kinases such as Akt and PDK1 which further modulate downstream effectors.

PKC zeta has associations with cancer and insulin resistance-related conditions. In cancer dysregulation of PKC zeta can lead to unchecked cellular proliferation due to its involvement in survival pathways and it often interacts with oncogenic proteins like Akt. In insulin resistance PKC zeta misregulation impacts glucose metabolism highlighting its role in diabetes-related pathways alongside insulin receptor substrates.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Phosphorylates VAMP2 in vitro (PubMed : 17313651). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed : 36040231).. Isoform 2. Involved in late synaptic long term potention phase in CA1 hippocampal cells and long term memory maintenance.

See full target information PRKCZ

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 16:5095-5109 PubMed38026263

2023

LPA Alleviates Septic Acute Lung Injury via Protective Endothelial Barrier Function Through Activation of PLC-PKC-FAK.

Applications

Unspecified application

Species

Unspecified reactive species

Ruifeng Bai,Jianqiu Pei,Shengqiang Pei,Xiangfeng Cong,Jerold Chun,Fang Wang,Xi Chen

Cell biology international 47:1327-1343 PubMed37191290

2023

HPV E6 promotes cell proliferation of cervical cancer cell by accelerating accumulation of RBM15 dependently of autophagy inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Nie,Bo Tang,Mingfen Lv,Danyang Li,Tian Li,Rongying Ou,Yunsheng Xu,Juan Wen

Breast cancer research : BCR 25:38 PubMed37029374

2023

PKC-ζ mediated reduction of the extracellular vesicles-associated TGF-β1 overcomes radiotherapy resistance in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fayun Zhang,Zifeng Zheng,Luoyang Wang,Wenfeng Zeng,Wenjing Wei,Chunling Zhang,Ziran Zhao,Wei Liang

European journal of histochemistry : EJH 66: PubMed36305269

2022

VEGF promotes diabetic retinopathy by upregulating the PKC/ET/NF-κB/ICAM-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Meiying Zhang,Min Zhou,Xia Cai,Yan Zhou,Xueling Jiang,Yan Luo,Yue Hu,Rong Qiu,Yanrong Wu,Yuejin Zhang,Yan Xiong

Frontiers in genetics 13:1034889 PubMed36330442

2022

G3BP1 regulates breast cancer cell proliferation and metastasis by modulating PKCζ.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Liu,Shaoping Tian,Tianyu Lin,Xin He,Justin Eze Ideozu,Rui Wang,Yong Wang,Dan Yue,Hua Geng

Frontiers in cellular neuroscience 16:860823 PubMed35783090

2022

Enhancement of Phosphorylation and Transport Activity of the Neuronal Glutamate Transporter Excitatory Amino Acid Transporter 3 by C3bot and a 26mer C3bot Peptide.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes Piepgras,Astrid Rohrbeck,Ingo Just,Stefan Bittner,Gudrun Ahnert-Hilger,Markus Höltje

Journal of cellular physiology 236:1913-1925 PubMed32740941

2020

HomeoboxC6 affects the apoptosis of human vascular endothelial cells and is involved in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangshu Long,Ganhua You,Qiang Wu,Yu Zhou,Yan Xiao,Fuxun Yu,Shiyan Deng,Rui Mo,Fang Song,Jing Huang,Maobo Tian

Cell metabolism 29:993-1002.e6 PubMed30527740

2018

Epithelial-Mesenchymal Transition Directs Stem Cell Polarity via Regulation of Mitofusin.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Ju Wu,Yu-Syuan Chen,Mi Ran Kim,Chao-Ching Chang,Silpa Gampala,Yingsheng Zhang,Yueyang Wang,Chih-Yu Chang,Jer-Yen Yang,Chun-Ju Chang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PKC zeta antibody [EP1490(2)]

Western blot - Anti-PKC zeta antibody [EP1490(2)] (AB108970)

Western blot - Anti-PKC zeta antibody [EP1490(2)] (AB108970)

Western blot - Anti-PKC zeta antibody [EP1490(2)] (AB108970)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise