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Rabbit Recombinant Monoclonal KPCZ phospho T410 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human, Recombinant full length protein - Human samples. Cited in 6 publications.

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Images

Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (AB76129), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (AB76129), expandable thumbnail
  • Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (AB76129), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (AB76129), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (AB76129), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPIHC-PWB
Human
Not recommended
Tested
Tested
Mouse
Not recommended
Tested
Tested
Rat
Not recommended
Tested
Expected
Recombinant full length protein - Human
Not recommended
Not recommended
Tested

Not recommended
Not recommended

Species

Rat, Mouse, Human, Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/20000

Notes

For unpurified, use 1/1000 - 1/2000.

Species

Human

Dilution info

1/20000

Notes

For unpurified, use 1/1000 - 1/2000.

Species

Recombinant full length protein - Human

Dilution info

1/20000

Notes

For unpurified, use 1/1000 - 1/2000.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Phosphorylates VAMP2 in vitro (PubMed:17313651). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed:36040231).Isoform 2Involved in late synaptic long term potention phase in CA1 hippocampal cells and long term memory maintenance.

Targets

Protein kinase C iota type phospho T412

Alternative names

Recommended products

Rabbit Recombinant Monoclonal KPCZ phospho T410 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human, Recombinant full length protein - Human samples. Cited in 6 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP1491Y

Purification technique

Affinity purification Protein A

Specificity

This antibody detects PKC zeta and PKC lamda phosphorylated at T410/412 respectively.
This antibody also detects various PKC phosphorylated isoforms.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Protein Kinase C zeta and iota (PKCζ and PKCι) also known as PRKCZ and PRKCI are important members of the atypical PKC subgroup within the larger PKC family. PKCζ and PKCι facilitate signal transduction through phosphorylation a process essential for modifying protein activity. These proteins have an approximate mass of 67 kDa. They are found in various tissues with expression in the brain lung and kidney being notably high. Both PKCζ and PKCι participate in cell processes by acting through the phosphorylation of specific substrate proteins.

Biological function summary

Atypical PKCs such as PKCζ and PKCι regulate numerous cellular activities including cell proliferation survival and differentiation. These proteins often exist as part of larger signaling complexes. For example PKCζ functions within a complex involving Par-6 and Par-3 playing an important role in maintaining cell polarity. PKCι similarly contributes to cellular processes essential for growth and development by interacting with several proteins involved in signaling cascades.

Pathways

PKCζ and PKCι integrate into signaling networks like the PI3K/Akt pathway which is critical for controlling cell growth and survival. These atypical PKCs do not need calcium or diacylglycerol for activation unlike other PKCs. They interact with proteins such as p62 and mTOR influencing cell metabolism and apoptosis. Through these interactions PKCζ and PKCι help modulate important cellular responses to extracellular signals.

Associated diseases and disorders

PKCζ and PKCι have established connections with conditions like cancer and metabolic syndrome. Dysregulation of PKCζ can contribute to tumorigenesis by affecting processes like apoptosis and cell cycle control. PKCι has links to lung cancer and is implicated in improper cell cycle regulation. During these conditions PKCζ and PKCι often show abnormal interactions with proteins such as Akt and mTOR highlighting their role in pathological processes affecting cell growth and metabolism.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129), expandable thumbnail

    Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129) at 1/20000 dilution

    All lanes: Mouse lung tissue lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 68 kDa

    Observed band size: 74 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)

    Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (HRP) ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129), expandable thumbnail

    Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)

    All lanes: Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129) at 1/2000 dilution

    Lane 1: 293 cell lysates, untreated at 10 µg

    Lane 2: 293 cell lysates, treated with AP at 10 µg

    Secondary

    All lanes: goat anti-rabbit-HRP at 1/1000 dilution

    Predicted band size: 68 kDa

    Observed band size: 165 kDa, 170 kDa, 68 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)

    Immunohistochemical staining of paraffin embedded mouse kidney with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (HRP) ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)

    Immunohistochemical staining of paraffin embedded human endometrium with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (HRP) ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129), expandable thumbnail

    Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129) at 1/20000 dilution

    Lane 1: Untreated HeLa cell lysate at 10 µg

    Lane 2: HeLa cell lysate treated with alkaline phosphatase at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 68 kDa

    Observed band size: 74 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)

    Immunohistochemical staining of PKC zeta in human brain with unpurified ab76129 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129), expandable thumbnail

    Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 180 seconds; Lane 5-6: 60 seconds; Lane 7-10: 5 seconds.

    All lanes: Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129) at 1/1000 dilution

    Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (Recombinant human PKC alpha protein (Active) ab55672)

    Lane 2: Western blot - Recombinant human PKC beta 1 protein (Recombinant human PKC beta 1 protein ab60840)

    Lane 3: Western blot - Recombinant human PKC beta 2 protein (Recombinant human PKC beta 2 protein ab60841)

    Lane 4: Western blot - Recombinant human PKC gamma protein (Recombinant human PKC gamma protein ab60842)

    Lane 5: Western blot - Recombinant human PKC delta protein (Recombinant human PKC delta protein ab60844)

    Lane 6: Western blot - Recombinant human PKC epsilon protein (Recombinant human PKC epsilon protein ab60847)

    Lane 7: Western blot - Recombinant human PKC zeta protein (Recombinant human PKC zeta protein ab60848)

    Lane 8: Western blot - Recombinant human PKC eta protein (Recombinant human PKC eta protein ab60849)

    Lane 9: Western blot - Recombinant human PKC theta/PRKCQ protein (Recombinant human PKC theta/PRKCQ protein ab56641)

    Lane 10: Western blot - Recombinant human PKC iota protein (Recombinant human PKC iota protein ab60850)

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 105 kDa

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Product protocols

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