Rabbit Recombinant Monoclonal KPCZ phospho T410 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human, Recombinant full length protein - Human samples. Cited in 6 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | IHC-P | WB | |
---|---|---|---|
Human | Not recommended | Tested | Tested |
Mouse | Not recommended | Tested | Tested |
Rat | Not recommended | Tested | Expected |
Recombinant full length protein - Human | Not recommended | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse, Human, Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/20000 | Notes For unpurified, use 1/1000 - 1/2000. |
Species Human | Dilution info 1/20000 | Notes For unpurified, use 1/1000 - 1/2000. |
Species Recombinant full length protein - Human | Dilution info 1/20000 | Notes For unpurified, use 1/1000 - 1/2000. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Phosphorylates VAMP2 in vitro (PubMed:17313651). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed:36040231).Isoform 2Involved in late synaptic long term potention phase in CA1 hippocampal cells and long term memory maintenance.
Protein kinase C iota type phospho T412
PKC2, PRKCZ, PKC2, Protein kinase C zeta type, nPKC-zeta
Rabbit Recombinant Monoclonal KPCZ phospho T410 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human, Recombinant full length protein - Human samples. Cited in 6 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EP1491Y
Affinity purification Protein A
This antibody detects PKC zeta and PKC lamda phosphorylated at T410/412 respectively.
This antibody also detects various PKC phosphorylated isoforms.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
Protein Kinase C zeta and iota (PKCζ and PKCι) also known as PRKCZ and PRKCI are important members of the atypical PKC subgroup within the larger PKC family. PKCζ and PKCι facilitate signal transduction through phosphorylation a process essential for modifying protein activity. These proteins have an approximate mass of 67 kDa. They are found in various tissues with expression in the brain lung and kidney being notably high. Both PKCζ and PKCι participate in cell processes by acting through the phosphorylation of specific substrate proteins.
Atypical PKCs such as PKCζ and PKCι regulate numerous cellular activities including cell proliferation survival and differentiation. These proteins often exist as part of larger signaling complexes. For example PKCζ functions within a complex involving Par-6 and Par-3 playing an important role in maintaining cell polarity. PKCι similarly contributes to cellular processes essential for growth and development by interacting with several proteins involved in signaling cascades.
PKCζ and PKCι integrate into signaling networks like the PI3K/Akt pathway which is critical for controlling cell growth and survival. These atypical PKCs do not need calcium or diacylglycerol for activation unlike other PKCs. They interact with proteins such as p62 and mTOR influencing cell metabolism and apoptosis. Through these interactions PKCζ and PKCι help modulate important cellular responses to extracellular signals.
PKCζ and PKCι have established connections with conditions like cancer and metabolic syndrome. Dysregulation of PKCζ can contribute to tumorigenesis by affecting processes like apoptosis and cell cycle control. PKCι has links to lung cancer and is implicated in improper cell cycle regulation. During these conditions PKCζ and PKCι often show abnormal interactions with proteins such as Akt and mTOR highlighting their role in pathological processes affecting cell growth and metabolism.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129) at 1/20000 dilution
All lanes: Mouse lung tissue lysate at 10 µg
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 74 kDa
Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (HRP) ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
All lanes: Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129) at 1/2000 dilution
Lane 1: 293 cell lysates, untreated at 10 µg
Lane 2: 293 cell lysates, treated with AP at 10 µg
All lanes: goat anti-rabbit-HRP at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 165 kDa, 170 kDa, 68 kDa
Immunohistochemical staining of paraffin embedded mouse kidney with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (HRP) ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunohistochemical staining of paraffin embedded human endometrium with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (HRP) ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129) at 1/20000 dilution
Lane 1: Untreated HeLa cell lysate at 10 µg
Lane 2: HeLa cell lysate treated with alkaline phosphatase at 10 µg
All lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 74 kDa
Immunohistochemical staining of PKC zeta in human brain with unpurified ab76129 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1-4: 180 seconds; Lane 5-6: 60 seconds; Lane 7-10: 5 seconds.
All lanes: Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (Recombinant human PKC alpha protein (Active) ab55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (Recombinant human PKC beta 1 protein ab60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (Recombinant human PKC beta 2 protein ab60841)
Lane 4: Western blot - Recombinant human PKC gamma protein (Recombinant human PKC gamma protein ab60842)
Lane 5: Western blot - Recombinant human PKC delta protein (Recombinant human PKC delta protein ab60844)
Lane 6: Western blot - Recombinant human PKC epsilon protein (Recombinant human PKC epsilon protein ab60847)
Lane 7: Western blot - Recombinant human PKC zeta protein (Recombinant human PKC zeta protein ab60848)
Lane 8: Western blot - Recombinant human PKC eta protein (Recombinant human PKC eta protein ab60849)
Lane 9: Western blot - Recombinant human PKC theta/PRKCQ protein (Recombinant human PKC theta/PRKCQ protein ab56641)
Lane 10: Western blot - Recombinant human PKC iota protein (Recombinant human PKC iota protein ab60850)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 105 kDa
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