JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB76129

Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y]

Be the first to review this product! Submit a review

|

(6 Publications)

Rabbit Recombinant Monoclonal KPCZ phospho T410 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human, Recombinant full length protein - Human samples. Cited in 6 publications.

View Alternative Names

PKC2, PRKCZ, Protein kinase C zeta type, nPKC-zeta, DXS1179E, PRKCI, Protein kinase C iota type, Atypical protein kinase C-lambda/iota, nPKC-iota, PRKC-lambda/iota, aPKC-lambda/iota

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (AB76129)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (AB76129)

Immunohistochemical staining of PKC zeta in human brain with unpurified ab76129 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (AB76129)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (AB76129)

Immunohistochemical staining of paraffin embedded human endometrium with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051) used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (AB76129)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (AB76129)

Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051) used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (AB76129)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (AB76129)

Immunohistochemical staining of paraffin embedded mouse kidney with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051) used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

Western blot - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (AB76129)
  • WB

Unknown

Western blot - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (AB76129)

All lanes:

Western blot - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (ab76129) at 1/2000 dilution

Lane 1:

293 cell lysates, untreated at 10 µg

Lane 2:

293 cell lysates, treated with AP at 10 µg

Secondary

All lanes:

goat anti-rabbit-HRP at 1/1000 dilution

Predicted band size: 68 kDa

Observed band size: 165 kDa,170 kDa,68 kDa

false

Western blot - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (AB76129)
  • WB

Lab

Western blot - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (AB76129)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (ab76129) at 1/20000 dilution

Lane 1:

Untreated HeLa cell lysate at 10 µg

Lane 2:

HeLa cell lysate treated with alkaline phosphatase at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 68 kDa

Observed band size: 74 kDa

false

Western blot - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (AB76129)
  • WB

Lab

Western blot - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (AB76129)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (ab76129) at 1/20000 dilution

All lanes:

Mouse lung tissue lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 68 kDa

Observed band size: 74 kDa

false

Western blot - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (AB76129)
  • WB

Supplier Data

Western blot - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (AB76129)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Exposure time : Lane 1-4 : 180 seconds; Lane 5-6 : 60 seconds; Lane 7-10 : 5 seconds.

All lanes:

Western blot - Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (ab76129) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC epsilon protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 7:

Western blot - Recombinant human PKC zeta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-zeta-protein-ab60848'>ab60848</a>)

Lane 8:

Western blot - Recombinant human PKC eta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 9:

Western blot - Recombinant human PKC theta/PRKCQ protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 10:

Western blot - Recombinant human PKC iota protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-iota-protein-ab60850'>ab60850</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 105 kDa

false

  • Carrier free

    Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1491Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects PKC zeta and PKC lamda phosphorylated at T410/412 respectively.
This antibody also detects various PKC phosphorylated isoforms.

style
left-column

Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
Primary Antibodies

AB108970

Anti-PKC zeta antibody [EP1490(2)]

RabMAb|KO Validated

Western blot - Anti-PKC zeta antibody [EP1490(2)] (AB108970)

  • 5
  • 1
Primary Antibodies

AB32199

Anti-PTEN antibody [Y184]

20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Western blot - Anti-PTEN antibody [Y184] (AB32199)

  • 5
  • 15
Primary Antibodies

AB138498

Anti-non-muscle Myosin IIA antibody [EPR8965]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIA antibody [EPR8965] (AB138498)

  • 5
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "<p><strong>For unpurified, use 1/1000 - 1/2000.</strong></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "<p><strong>For unpurified, use 1/1000 - 1/2000.</strong></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "<p><strong>For unpurified, use 1/1000 - 1/2000.</strong></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein Kinase C zeta and iota (PKCζ and PKCι) also known as PRKCZ and PRKCI are important members of the atypical PKC subgroup within the larger PKC family. PKCζ and PKCι facilitate signal transduction through phosphorylation a process essential for modifying protein activity. These proteins have an approximate mass of 67 kDa. They are found in various tissues with expression in the brain lung and kidney being notably high. Both PKCζ and PKCι participate in cell processes by acting through the phosphorylation of specific substrate proteins.
Biological function summary

Atypical PKCs such as PKCζ and PKCι regulate numerous cellular activities including cell proliferation survival and differentiation. These proteins often exist as part of larger signaling complexes. For example PKCζ functions within a complex involving Par-6 and Par-3 playing an important role in maintaining cell polarity. PKCι similarly contributes to cellular processes essential for growth and development by interacting with several proteins involved in signaling cascades.

Pathways

PKCζ and PKCι integrate into signaling networks like the PI3K/Akt pathway which is critical for controlling cell growth and survival. These atypical PKCs do not need calcium or diacylglycerol for activation unlike other PKCs. They interact with proteins such as p62 and mTOR influencing cell metabolism and apoptosis. Through these interactions PKCζ and PKCι help modulate important cellular responses to extracellular signals.

PKCζ and PKCι have established connections with conditions like cancer and metabolic syndrome. Dysregulation of PKCζ can contribute to tumorigenesis by affecting processes like apoptosis and cell cycle control. PKCι has links to lung cancer and is implicated in improper cell cycle regulation. During these conditions PKCζ and PKCι often show abnormal interactions with proteins such as Akt and mTOR highlighting their role in pathological processes affecting cell growth and metabolism.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Phosphorylates VAMP2 in vitro (PubMed : 17313651). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed : 36040231).. Isoform 2. Involved in late synaptic long term potention phase in CA1 hippocampal cells and long term memory maintenance.
See full target information PRKCZ pT410
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 11:1231489 PubMed37635877

2023

Nox1-based NADPH oxidase regulates the Par protein complex activity to control cell polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandra Valdivia,Charity Duran,Mingyoung Lee,Holly C Williams,Moo-Yeol Lee,Alejandra San Martin

Breast cancer research : BCR 25:38 PubMed37029374

2023

PKC-ζ mediated reduction of the extracellular vesicles-associated TGF-β1 overcomes radiotherapy resistance in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fayun Zhang,Zifeng Zheng,Luoyang Wang,Wenfeng Zeng,Wenjing Wei,Chunling Zhang,Ziran Zhao,Wei Liang

Frontiers in nutrition 9:853225 PubMed35356725

2022

Berry Polyphenols Extract Alleviates Exercise Fatigue in Mice by Reducing Oxidative Stress, Inflammation, Skeletal Muscle Cell Apoptosis, and by Increasing Cell Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Suwen Liu,Fanna Meng,Dong Zhang,Donglin Shi,Junyi Zhou,Shuo Guo,Xuedong Chang

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33:12019-12035 PubMed31373844

2019

Attenuation of amyloid-β generation by atypical protein kinase C-mediated phosphorylation of engulfment adaptor PTB domain containing 1 threonine 35.

Applications

Unspecified application

Species

Unspecified reactive species

Dennis Dik-Long Chau,Kristen Wing-Yu Yung,William Wai-Lun Chan,Ying An,Yan Hao,Ho-Yin Edwin Chan,Jacky Chi-Ki Ngo,Kwok-Fai Lau

Journal of Cancer 8:3371-3377 PubMed29158810

2017

Phosphorylated Protein Kinase C (Zeta/Lambda) Expression in Colorectal Adenocarcinoma and Its Correlation with Clinicopathologic Characteristics and Prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Kyung Yeo,Ji Yeon Kim,In-Ock Seong,Jin-Man Kim,Kyung-Hee Kim

PloS one 7:e34596 PubMed22506032

2012

MicroRNAs overexpressed in growth-restricted rat skeletal muscles regulate the glucose transport in cell culture targeting central TGF-β factor SMAD4.

Applications

WB

Species

Rat

Santanu Raychaudhuri
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com