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Rabbit Recombinant Monoclonal KPCZ phospho T560 antibody. Suitable for Dot, WB, IHC-P and reacts with Synthetic peptide, Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 22 publications.

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Images

Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372), expandable thumbnail
  • Dot Blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372), expandable thumbnail
  • Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow CytDotWBIHC-P
Human
Not recommended
Not recommended
Expected
Tested
Tested
Mouse
Not recommended
Not recommended
Expected
Expected
Expected
Rat
Not recommended
Not recommended
Expected
Expected
Expected
Recombinant full length protein - Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Synthetic peptide
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species

Mouse, Rat, Human, Synthetic peptide, Recombinant full length protein - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human, Synthetic peptide, Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Species

Recombinant full length protein - Human

Dilution info

1/1000 - 1/10000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Synthetic peptide, Recombinant full length protein - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Phosphorylates VAMP2 in vitro (PubMed:17313651). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed:36040231).Isoform 2Involved in late synaptic long term potention phase in CA1 hippocampal cells and long term memory maintenance.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal KPCZ phospho T560 antibody. Suitable for Dot, WB, IHC-P and reacts with Synthetic peptide, Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 22 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP2037AY

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PKC zeta also known as Protein Kinase C zeta is a member of the atypical protein kinase C family. This enzyme plays an important role in various cellular processes. PKC zeta has a molecular mass of about 55 kDa and is expressed in many tissues including brain and immune cells. Unlike conventional PKC enzymes PKC zeta does not respond to calcium or diacylglycerol which influences its unique regulatory roles.

Biological function summary

PKC zeta participates in regulating cell proliferation survival and differentiation. It often functions as part of signaling complexes that include other proteins like PDK1 and is involved in transducing signals from cell surface receptors to the nucleus. Its activity influences cellular responses to hormones growth factors and cytokines contributing to diverse cellular events.

Pathways

PKC zeta plays a critical role in the NF-kappaB and PI3K/Akt signaling pathways. In the NF-kappaB pathway PKC zeta helps in the activation of this transcription factor affecting genes related to inflammation and immune response. In the PI3K/Akt pathway it is involved in cell growth and survival interacting with kinases such as Akt and PDK1 which further modulate downstream effectors.

Associated diseases and disorders

PKC zeta has associations with cancer and insulin resistance-related conditions. In cancer dysregulation of PKC zeta can lead to unchecked cellular proliferation due to its involvement in survival pathways and it often interacts with oncogenic proteins like Akt. In insulin resistance PKC zeta misregulation impacts glucose metabolism highlighting its role in diabetes-related pathways alongside insulin receptor substrates.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372), expandable thumbnail

    Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372) at 1/20000 dilution

    Lane 1: HeLa cell lysate - treated with Calyculin A at 10 µg

    Lane 2: HeLa cell lysate - treated with Calyculin A and lambda phosphatase at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 68 kDa

    Exposure time: 3min

  • Dot Blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372), expandable thumbnail

    Dot Blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

    Dot blot analysis of PKC zeta (pT560) peptide (Lane 1) and PKC zeta non-phospho peptide (Lane 2) labelling PKC zeta (pT560) with ab62372 at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

    ab62372 at 1/100 dilution staining PKC zeta in human breast ductal carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372), expandable thumbnail

    Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

    All lanes: Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

    Lane 1: Hela Cell Lysate (untreated with Calyculin A)

    Lane 2: Hela Cell Lysate (Treated with Calyculin A)

    Predicted band size: 68 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

    ab62372 at 1/100 dilution staining PKC zeta in human brain by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372), expandable thumbnail

    Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372) at 1/1000 dilution

    Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (Recombinant human PKC alpha protein (Active) ab55672)

    Lane 2: Western blot - Recombinant human PKC beta 1 protein (Recombinant human PKC beta 1 protein ab60840)

    Lane 3: Western blot - Recombinant human PKC beta 2 protein (Recombinant human PKC beta 2 protein ab60841)

    Lane 4: Western blot - Recombinant human PKC gamma protein (Recombinant human PKC gamma protein ab60842)

    Lane 5: Western blot - Recombinant human PKC delta protein (Recombinant human PKC delta protein ab60844)

    Lane 6: Western blot - Recombinant human PKC epsilon protein (Recombinant human PKC epsilon protein ab60847)

    Lane 7: Western blot - Recombinant human PKC zeta protein (Recombinant human PKC zeta protein ab60848)

    Lane 8: Western blot - Recombinant human PKC eta protein (Recombinant human PKC eta protein ab60849)

    Lane 9: Western blot - Recombinant human PKC theta/PRKCQ protein (Recombinant human PKC theta/PRKCQ protein ab56641)

    Lane 10: Western blot - Recombinant human PKC iota protein (Recombinant human PKC iota protein ab60850)

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 68 kDa

    Observed band size: 105 kDa

    Exposure time: 5s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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