AB62372

Anti-PKC zeta (phospho T560) antibody [EP2037AY]

RabMAb
Recombinant
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(24 Publications)

Rabbit Recombinant Monoclonal KPCZ phospho T560 antibody. Suitable for Dot, WB, IHC-P and reacts with Synthetic peptide, Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 24 publications.

View Alternative Names

PKC2, PRKCZ, Protein kinase C zeta type, nPKC-zeta

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372)

ab62372 at 1/100 dilution staining PKC zeta in human brain by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372)

ab62372 at 1/100 dilution staining PKC zeta in human breast ductal carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Lab

Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372) at 1/20000 dilution

Lane 1:

HeLa cell lysate - treated with Calyculin A at 10 µg

Lane 2:

HeLa cell lysate - treated with Calyculin A and lambda phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 68 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC gamma protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>)

Lane 5:

Western blot - Recombinant human PKC delta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 6:

Western blot - Recombinant human PKC epsilon protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 7:

Western blot - Recombinant human PKC zeta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-zeta-protein-ab60848'>ab60848</a>)

Lane 8:

Western blot - Recombinant human PKC eta protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 9:

Western blot - Recombinant human PKC theta/PRKCQ protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 10:

Western blot - Recombinant human PKC iota protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pkc-iota-protein-ab60850'>ab60850</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 68 kDa

Observed band size: 105 kDa

false

Exposure time: 5s

Unknown

Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372)

All lanes:

Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)

Lane 1:

Hela Cell Lysate (untreated with Calyculin A)

Lane 2:

Hela Cell Lysate (Treated with Calyculin A)

Predicted band size: 68 kDa

false

Unknown

Dot Blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372)

Dot blot analysis of PKC zeta (pT560) peptide (Lane 1) and PKC zeta non-phospho peptide (Lane 2) labelling PKC zeta (pT560) with ab62372 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Carrier free

Anti-PKC zeta (phospho T560) antibody [EP2037AY] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2037AY

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Mouse

Applications

IHC-P, WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PKC zeta also known as Protein Kinase C zeta is a member of the atypical protein kinase C family. This enzyme plays an important role in various cellular processes. PKC zeta has a molecular mass of about 55 kDa and is expressed in many tissues including brain and immune cells. Unlike conventional PKC enzymes PKC zeta does not respond to calcium or diacylglycerol which influences its unique regulatory roles.

Biological function summary

PKC zeta participates in regulating cell proliferation survival and differentiation. It often functions as part of signaling complexes that include other proteins like PDK1 and is involved in transducing signals from cell surface receptors to the nucleus. Its activity influences cellular responses to hormones growth factors and cytokines contributing to diverse cellular events.

Pathways

PKC zeta plays a critical role in the NF-kappaB and PI3K/Akt signaling pathways. In the NF-kappaB pathway PKC zeta helps in the activation of this transcription factor affecting genes related to inflammation and immune response. In the PI3K/Akt pathway it is involved in cell growth and survival interacting with kinases such as Akt and PDK1 which further modulate downstream effectors.

PKC zeta has associations with cancer and insulin resistance-related conditions. In cancer dysregulation of PKC zeta can lead to unchecked cellular proliferation due to its involvement in survival pathways and it often interacts with oncogenic proteins like Akt. In insulin resistance PKC zeta misregulation impacts glucose metabolism highlighting its role in diabetes-related pathways alongside insulin receptor substrates.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Phosphorylates VAMP2 in vitro (PubMed : 17313651). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed : 36040231).. Isoform 2. Involved in late synaptic long term potention phase in CA1 hippocampal cells and long term memory maintenance.

See full target information PRKCZ pT560

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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10541 PubMed39627191

2024

Cell polarity proteins promote macropinocytosis in response to metabolic stress.

Applications

Unspecified application

Species

Unspecified reactive species

Guillem Lambies,Szu-Wei Lee,Karen Duong-Polk,Pedro Aza-Blanc,Swetha Maganti,Cheska M Galapate,Anagha Deshpande,Aniruddha J Deshpande,David A Scott,David W Dawson,Cosimo Commisso

Frontiers in genetics 13:1034889 PubMed36330442

2022

G3BP1 regulates breast cancer cell proliferation and metastasis by modulating PKCζ.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Liu,Shaoping Tian,Tianyu Lin,Xin He,Justin Eze Ideozu,Rui Wang,Yong Wang,Dan Yue,Hua Geng

International journal of molecular sciences 23: PubMed35562872

2022

Rap1a Activity Elevated the Impact of Endogenous AGEs in Diabetic Collagen to Stimulate Increased Myofibroblast Transition and Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie D Burr,Christopher C Dorroh,James A Stewart

Cells 10: PubMed33806572

2021

Rap1a Overlaps the AGE/RAGE Signaling Cascade to Alter Expression of α-SMA, p-NF-κB, and p-PKC-ζ in Cardiac Fibroblasts Isolated from Type 2 Diabetic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie D Burr,James A Stewart

Experimental dermatology 30:358-366 PubMed33617094

2021

MTOR promotes basal cell carcinoma growth through atypical PKC.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel Y Chow,Taylor M Levee,Gurleen Kaur,Daniel P Cedeno,Linda T Doan,Scott X Atwood

Journal of cellular physiology 236:1913-1925 PubMed32740941

2020

HomeoboxC6 affects the apoptosis of human vascular endothelial cells and is involved in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangshu Long,Ganhua You,Qiang Wu,Yu Zhou,Yan Xiao,Fuxun Yu,Shiyan Deng,Rui Mo,Fang Song,Jing Huang,Maobo Tian

Cell 181:1596-1611.e27 PubMed32559461

2020

Metabolic Fingerprinting Links Oncogenic PIK3CA with Enhanced Arachidonic Acid-Derived Eicosanoids.

Applications

Unspecified application

Species

Unspecified reactive species

Nikos Koundouros,Evdoxia Karali,Aurelien Tripp,Adamo Valle,Paolo Inglese,Nicholas J S Perry,David J Magee,Sara Anjomani Virmouni,George A Elder,Adam L Tyson,Maria Luisa Dória,Antoinette van Weverwijk,Renata F Soares,Clare M Isacke,Jeremy K Nicholson,Robert C Glen,Zoltan Takats,George Poulogiannis

Current biology : CB 30:893-898.e5 PubMed32084408

2020

A Conserved PDZ-Binding Motif in aPKC Interacts with Par-3 and Mediates Cortical Polarity.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan W Holly,Kimberly Jones,Kenneth E Prehoda

International journal of molecular sciences 21: PubMed31947656

2020

Enteropathogenic (EPEC) Recruitment of PAR Polarity Protein Atypical PKCζ to Pedestals and Cell-Cell Contacts Precedes Disruption of Tight Junctions in Intestinal Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rocio Tapia,Sarah E Kralicek,Gail A Hecht

Nature cell biology 21:359-371 PubMed30804505

2019

Apical-basal polarity inhibits epithelial-mesenchymal transition and tumour metastasis by PAR-complex-mediated SNAI1 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Hae-Yun Jung,Laurent Fattet,Jeff H Tsai,Taketoshi Kajimoto,Qiang Chang,Alexandra C Newton,Jing Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PKC zeta (phospho T560) antibody [EP2037AY]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372)

Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372)

Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372)

Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372)

Dot Blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (AB62372)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (24)

Product promise