Rabbit Recombinant Monoclonal KPCZ phospho T560 antibody. Suitable for Dot, WB, IHC-P and reacts with Synthetic peptide, Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 22 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
Liquid
Monoclonal
ICC/IF | Flow Cyt | Dot | WB | IHC-P | |
---|---|---|---|---|---|
Human | Not recommended | Not recommended | Expected | Tested | Tested |
Mouse | Not recommended | Not recommended | Expected | Expected | Expected |
Rat | Not recommended | Not recommended | Expected | Expected | Expected |
Recombinant full length protein - Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human, Synthetic peptide, Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human, Synthetic peptide, Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Recombinant full length protein - Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide, Recombinant full length protein - Human | Dilution info - | Notes - |
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Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Phosphorylates VAMP2 in vitro (PubMed:17313651). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed:36040231).Isoform 2Involved in late synaptic long term potention phase in CA1 hippocampal cells and long term memory maintenance.
PKC2, PKC2, PRKCZ, Protein kinase C zeta type, nPKC-zeta
Rabbit Recombinant Monoclonal KPCZ phospho T560 antibody. Suitable for Dot, WB, IHC-P and reacts with Synthetic peptide, Human, Mouse, Rat, Recombinant full length protein - Human samples. Cited in 22 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
Liquid
Monoclonal
EP2037AY
Affinity purification Protein A
Blue Ice
+4°C
-20°C
Stable for 12 months at -20°C
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
PKC zeta also known as Protein Kinase C zeta is a member of the atypical protein kinase C family. This enzyme plays an important role in various cellular processes. PKC zeta has a molecular mass of about 55 kDa and is expressed in many tissues including brain and immune cells. Unlike conventional PKC enzymes PKC zeta does not respond to calcium or diacylglycerol which influences its unique regulatory roles.
PKC zeta participates in regulating cell proliferation survival and differentiation. It often functions as part of signaling complexes that include other proteins like PDK1 and is involved in transducing signals from cell surface receptors to the nucleus. Its activity influences cellular responses to hormones growth factors and cytokines contributing to diverse cellular events.
PKC zeta plays a critical role in the NF-kappaB and PI3K/Akt signaling pathways. In the NF-kappaB pathway PKC zeta helps in the activation of this transcription factor affecting genes related to inflammation and immune response. In the PI3K/Akt pathway it is involved in cell growth and survival interacting with kinases such as Akt and PDK1 which further modulate downstream effectors.
PKC zeta has associations with cancer and insulin resistance-related conditions. In cancer dysregulation of PKC zeta can lead to unchecked cellular proliferation due to its involvement in survival pathways and it often interacts with oncogenic proteins like Akt. In insulin resistance PKC zeta misregulation impacts glucose metabolism highlighting its role in diabetes-related pathways alongside insulin receptor substrates.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372) at 1/20000 dilution
Lane 1: HeLa cell lysate - treated with Calyculin A at 10 µg
Lane 2: HeLa cell lysate - treated with Calyculin A and lambda phosphatase at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 68 kDa
Exposure time: 3min
Dot blot analysis of PKC zeta (pT560) peptide (Lane 1) and PKC zeta non-phospho peptide (Lane 2) labelling PKC zeta (pT560) with ab62372 at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
ab62372 at 1/100 dilution staining PKC zeta in human breast ductal carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
All lanes: Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)
Lane 1: Hela Cell Lysate (untreated with Calyculin A)
Lane 2: Hela Cell Lysate (Treated with Calyculin A)
Predicted band size: 68 kDa
ab62372 at 1/100 dilution staining PKC zeta in human brain by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372) at 1/1000 dilution
Lane 1: Western blot - Recombinant human PKC alpha protein (Active) (Recombinant human PKC alpha protein (Active) ab55672)
Lane 2: Western blot - Recombinant human PKC beta 1 protein (Recombinant human PKC beta 1 protein ab60840)
Lane 3: Western blot - Recombinant human PKC beta 2 protein (Recombinant human PKC beta 2 protein ab60841)
Lane 4: Western blot - Recombinant human PKC gamma protein (Recombinant human PKC gamma protein ab60842)
Lane 5: Western blot - Recombinant human PKC delta protein (Recombinant human PKC delta protein ab60844)
Lane 6: Western blot - Recombinant human PKC epsilon protein (Recombinant human PKC epsilon protein ab60847)
Lane 7: Western blot - Recombinant human PKC zeta protein (Recombinant human PKC zeta protein ab60848)
Lane 8: Western blot - Recombinant human PKC eta protein (Recombinant human PKC eta protein ab60849)
Lane 9: Western blot - Recombinant human PKC theta/PRKCQ protein (Recombinant human PKC theta/PRKCQ protein ab56641)
Lane 10: Western blot - Recombinant human PKC iota protein (Recombinant human PKC iota protein ab60850)
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 105 kDa
Exposure time: 5s
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