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AB153917

Anti-PKE antibody

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(1 Publication)

Rabbit Polyclonal PKE antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human STK32C aa 100-450.

View Alternative Names

YANK3, STK32C, Serine/threonine-protein kinase 32C, PKE, Yet another novel kinase 3

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-PKE antibody (AB153917)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PKE antibody (AB153917)

Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling PKE with ab153917 at 1/100 dilution. Lower panel is merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKE antibody (AB153917)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKE antibody (AB153917)

Immunohistochemical analysis of paraffin-embedded A549 xenograft labeling PKE with ab153917 at 1/500 dilution.

Western blot - Anti-PKE antibody (AB153917)
  • WB

Unknown

Western blot - Anti-PKE antibody (AB153917)

7.5% SDS PAGE

All lanes:

Western blot - Anti-PKE antibody (ab153917) at 1/500 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 55 kDa

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Western blot - Anti-PKE antibody (AB153917)
  • WB

Unknown

Western blot - Anti-PKE antibody (AB153917)

10% SDS PAGE

All lanes:

Western blot - Anti-PKE antibody (ab153917) at 1/10000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human STK32C aa 100-450. The exact immunogen used to generate this antibody is proprietary information.

Q86UX6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PKE also known as Protein Kinase Epsilon is a serine/threonine kinase with a mass of approximately 82 kDa. It belongs to the protein kinase C (PKC) family specifically the epsilon isoform. PKE expresses in a variety of tissues including brain heart and lungs where it plays a role in signal transduction processes. It achieves these functions through phosphorylation of target proteins modifying their activity localization and overall function.
Biological function summary

PKE influences cellular signaling and regulates important aspects of cell survival proliferation and differentiation. It associates with other proteins to form complexes that drive intracellular activities. PKE also takes part in neuron-related processes contributing significantly to maintenance of cellular integrity. Its activity affects cellular responses to extrinsic stimuli regulating pathways affecting diverse cellular outcomes.

Pathways

PKE integrates within signaling cascades such as the MAPK/ERK and NF-kB pathways. These pathways influence important cellular processes including growth and inflammation. In these systems PKE interacts with proteins like RAF1 and IKBKB driving downstream effects that alter gene expression and cellular responses. This involvement in such pathways highlights PKE's role in orchestrating complex biological processes.

PKE has implications in cancer and cardiac disorders. In cancer PKE's altered expression or activity impacts cell proliferation and survival interacting with other proteins such as RAS and AKT to promote oncogenic signaling. In cardiac disorders PKE modulates heart function and response to stress often implicating proteins such as TNNT2. Understanding PKE's role in these conditions aids in the development of targeted therapies that modulate its activity for therapeutic gain.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information STK32C
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40801656

2025

Notch2 Deletion Compromises Epithelial Integrity and Enamel Formation in Rodent Incisors.

Applications

Unspecified application

Species

Unspecified reactive species

Argyro Lamprou,Cristina Porcheri,Thimios A Mitsiadis
View all publications
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Product promise

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