AB204951

Anti-PKHD1 antibody [8G12A1]

Name: Anti-PKHD1 antibody [8G12A1] (ab204951) | Abcam
Brand: Abcam Limited
SKU: AB204951
Rating: 5 (1 reviews)

(1 Review)

(4 Publications)

Rat Monoclonal PKHD1 antibody. Suitable for Flow Cyt, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

FCYT, TIGM1, PKHD1, Fibrocystin, Polycystic kidney and hepatic disease 1 protein, Polyductin, Tigmin

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKHD1 antibody [8G12A1] (AB204951)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKHD1 antibody [8G12A1] (AB204951)

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissue labeling PKHD1 with ab204951 at 1/200 dilution followed by DAB staining

Immunocytochemistry/ Immunofluorescence - Anti-PKHD1 antibody [8G12A1] (AB204951)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PKHD1 antibody [8G12A1] (AB204951)

Immunofluorescent analysis of A431 cells labeling PKHD1 with ab204951 at 1/200 (green). Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Flow Cytometry - Anti-PKHD1 antibody [8G12A1] (AB204951)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-PKHD1 antibody [8G12A1] (AB204951)

Flow cytometric analysis of HeLa cells labeling PKHD1 with ab204951 at 1/200 (green) or negative control (red)

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

8G12A1

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt, ICC/IF, IHC-P

applications

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Reactivity data

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Product details

This product was changed from ascites to supernatant. Lot no's high than GR291788-5 are from Tissue Culture Supernatant

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PKHD1 gene encodes the polycystic kidney and hepatic disease 1 protein also referred to as fibrocystin/polyductin. This protein has a molecular mass of approximately 500 kDa. PKHD1 predominantly expresses in kidney and liver epithelial cells. In these tissues it localizes to the primary cilia and the plasma membrane. The structure of PKHD1 suggests it functions as a receptor or co-receptor involved in signal transduction.

Biological function summary

The PKHD1 protein plays a vital role in maintaining tubular and ductal architecture within the kidney and liver. It likely forms part of a complex allowing proper cellular communication and structural support. PKHD1 interacts with the cellular cytoskeleton suggesting a role in mechanosensation and cell proliferation. These functions contribute to normal tissue development and homeostasis in organs where it is expressed.

Pathways

PKHD1 plays a significant role in the Wnt signaling pathway and ciliary function. These pathways are important for regulating cell division polarity and organization. Within the Wnt signaling pathway PKHD1 interacts with proteins such as β-catenin influencing their stability and translocation. PKHD1's function in cilia suggests a link to cellular processes that depend on precise signal reception and transduction.

Mutations in PKHD1 are related to autosomal recessive polycystic kidney disease (ARPKD) and congenital hepatic fibrosis. These conditions involve the disrupted function of the kidneys and liver characterized by cyst formation and fibrosis. PKHD1's interaction with fibrocystin/polyductin is key as defects in this pathway lead to the abnormalities observed in ARPKD. Another protein fibrocystin shares overlapping functions with PKHD1 in these pathological conditions further emphasizing its critical role in maintaining proper kidney and liver function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Promotes ciliogenesis in renal epithelial cells and therefore participates in the tubules formation and/ or ensures the maintenance of the architecture of the lumen of the kidney (By similarity). Has an impact on cellular symmetry by ensuring correct bipolar cell division through the regulation of centrosome duplication and mitotic spindle assembly and by maintaining oriented cell division (OCD) during tubular elongation through planar cell polarity (PCP) pathway (PubMed : 20554582). During epithelial cell morphogenesis, it also regulates cell-cell and cell-matrix adhesion and participates in cell motility (By similarity). Promotes cell-cell contact through the positive regulation of PTK2 kinase activity leading to either positive regulation of epithelial cell proliferation through the HRAS/RAF1 pathways, or negative regulation of apoptosis through the PDK1/AKT1 pathway (By similarity). May act in collecting-duct and biliary differentiation (PubMed : 11919560). May participate in the regulation of the cholangiocytes proliferation and the CCN2 production in an CXCL8-dependent manner (PubMed : 30898581).

See full target information PKHD1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 33:357-367 PubMed39866229

2025

Melanoma cell line-derived exosomal miR-424-5p: a key promoter of angiogenesis through LATS2 interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Junwei DU,Qiang Zhang,Jing Zhang,Maierdanjiang Maihemuti,Haiyang He,Renbing Jiang

Journal of nanobiotechnology 22:177 PubMed38609995

2024

HWJMSC-EVs promote cartilage regeneration and repair via the ITGB1/TGF-β/Smad2/3 axis mediated by microfractures.

Applications

Unspecified application

Species

Unspecified reactive species

Zhian Chen,Tianhua Zhou,Huan Luo,Zhen Wang,Qiang Wang,Rongmao Shi,Zian Li,Rongqing Pang,Hongbo Tan

Science advances 8:eabq0866 PubMed36129975

2022

Organoid-on-a-chip model of human ARPKD reveals mechanosensing pathomechanisms for drug discovery.

Applications

Unspecified application

Species

Unspecified reactive species

Ken Hiratsuka,Tomoya Miyoshi,Katharina T Kroll,Navin R Gupta,M Todd Valerius,Thomas Ferrante,Michifumi Yamashita,Jennifer A Lewis,Ryuji Morizane

Tissue engineering and regenerative medicine 18:651-662 PubMed34165777

2021

Rapid Differentiation of Human Embryonic Stem Cells into Testosterone-Producing Leydig Cell-Like Cells In vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Young Shin,Seah Park,Won Yun Choi,Dong Ryul Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PKHD1 antibody [8G12A1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKHD1 antibody [8G12A1] (AB204951)

Immunocytochemistry/ Immunofluorescence - Anti-PKHD1 antibody [8G12A1] (AB204951)

Flow Cytometry - Anti-PKHD1 antibody [8G12A1] (AB204951)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Alternative Products

Primary Antibodies

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise