Rabbit Polyclonal PKHD1 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PKHD1 aa 2550-2700.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PKHD1 aa 2550-2700. The exact immunogen used to generate this antibody is proprietary information. Database link P08F94
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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Target data
Function
Promotes ciliogenesis in renal epithelial cells and therefore participates in the tubules formation and/ or ensures the maintenance of the architecture of the lumen of the kidney (By similarity). Has an impact on cellular symmetry by ensuring correct bipolar cell division through the regulation of centrosome duplication and mitotic spindle assembly and by maintaining oriented cell division (OCD) during tubular elongation through planar cell polarity (PCP) pathway (PubMed:20554582). During epithelial cell morphogenesis regulates also cell-cell and cell-matrix adhesion and participates in cell motility (By similarity). Promotes cell-cell contact through the positive regulation of PTK2 kinase activity leading to either positive regulation of epithelial cell proliferation through the HRAS/RAF1 pathways, or negative regulation of apoptosis through the PDK1/AKT1 pathway (By similarity). May act in collecting-duct and biliary differentiation (PubMed:11919560). May participate in the regulation of the cholangiocytes proliferation and the CCN2 production in an CXCL8-dependent manner (PubMed:30898581).
Alternative names
FCYT, TIGM1, PKHD1, Fibrocystin, Polycystic kidney and hepatic disease 1 protein, Polyductin, Tigmin
Recommended products
Rabbit Polyclonal PKHD1 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PKHD1 aa 2550-2700.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PKHD1 aa 2550-2700. The exact immunogen used to generate this antibody is proprietary information. Database link P08F94
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PKHD1 gene encodes the polycystic kidney and hepatic disease 1 protein also referred to as fibrocystin/polyductin. This protein has a molecular mass of approximately 500 kDa. PKHD1 predominantly expresses in kidney and liver epithelial cells. In these tissues it localizes to the primary cilia and the plasma membrane. The structure of PKHD1 suggests it functions as a receptor or co-receptor involved in signal transduction.
- Biological function summary
The PKHD1 protein plays a vital role in maintaining tubular and ductal architecture within the kidney and liver. It likely forms part of a complex allowing proper cellular communication and structural support. PKHD1 interacts with the cellular cytoskeleton suggesting a role in mechanosensation and cell proliferation. These functions contribute to normal tissue development and homeostasis in organs where it is expressed.
- Pathways
PKHD1 plays a significant role in the Wnt signaling pathway and ciliary function. These pathways are important for regulating cell division polarity and organization. Within the Wnt signaling pathway PKHD1 interacts with proteins such as β-catenin influencing their stability and translocation. PKHD1's function in cilia suggests a link to cellular processes that depend on precise signal reception and transduction.
- Associated diseases and disorders
Mutations in PKHD1 are related to autosomal recessive polycystic kidney disease (ARPKD) and congenital hepatic fibrosis. These conditions involve the disrupted function of the kidneys and liver characterized by cyst formation and fibrosis. PKHD1's interaction with fibrocystin/polyductin is key as defects in this pathway lead to the abnormalities observed in ARPKD. Another protein fibrocystin shares overlapping functions with PKHD1 in these pathological conditions further emphasizing its critical role in maintaining proper kidney and liver function.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKHD1 antibody (ab122160)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PKHD1 with ab122160. Staining shows strong cytoplasmic positivity in cells in tubules.
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