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AB122160

Anti-PKHD1 antibody

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(1 Publication)

Rabbit Polyclonal PKHD1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PKHD1 aa 2550-2700.

View Alternative Names

FCYT, TIGM1, PKHD1, Fibrocystin, Polycystic kidney and hepatic disease 1 protein, Polyductin, Tigmin

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKHD1 antibody (AB122160)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKHD1 antibody (AB122160)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PKHD1 with ab122160. Staining shows strong cytoplasmic positivity in cells in tubules.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PKHD1 aa 2550-2700. The exact immunogen used to generate this antibody is proprietary information.

P08F94

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PKHD1 gene encodes the polycystic kidney and hepatic disease 1 protein also referred to as fibrocystin/polyductin. This protein has a molecular mass of approximately 500 kDa. PKHD1 predominantly expresses in kidney and liver epithelial cells. In these tissues it localizes to the primary cilia and the plasma membrane. The structure of PKHD1 suggests it functions as a receptor or co-receptor involved in signal transduction.
Biological function summary

The PKHD1 protein plays a vital role in maintaining tubular and ductal architecture within the kidney and liver. It likely forms part of a complex allowing proper cellular communication and structural support. PKHD1 interacts with the cellular cytoskeleton suggesting a role in mechanosensation and cell proliferation. These functions contribute to normal tissue development and homeostasis in organs where it is expressed.

Pathways

PKHD1 plays a significant role in the Wnt signaling pathway and ciliary function. These pathways are important for regulating cell division polarity and organization. Within the Wnt signaling pathway PKHD1 interacts with proteins such as β-catenin influencing their stability and translocation. PKHD1's function in cilia suggests a link to cellular processes that depend on precise signal reception and transduction.

Mutations in PKHD1 are related to autosomal recessive polycystic kidney disease (ARPKD) and congenital hepatic fibrosis. These conditions involve the disrupted function of the kidneys and liver characterized by cyst formation and fibrosis. PKHD1's interaction with fibrocystin/polyductin is key as defects in this pathway lead to the abnormalities observed in ARPKD. Another protein fibrocystin shares overlapping functions with PKHD1 in these pathological conditions further emphasizing its critical role in maintaining proper kidney and liver function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes ciliogenesis in renal epithelial cells and therefore participates in the tubules formation and/ or ensures the maintenance of the architecture of the lumen of the kidney (By similarity). Has an impact on cellular symmetry by ensuring correct bipolar cell division through the regulation of centrosome duplication and mitotic spindle assembly and by maintaining oriented cell division (OCD) during tubular elongation through planar cell polarity (PCP) pathway (PubMed : 20554582). During epithelial cell morphogenesis, it also regulates cell-cell and cell-matrix adhesion and participates in cell motility (By similarity). Promotes cell-cell contact through the positive regulation of PTK2 kinase activity leading to either positive regulation of epithelial cell proliferation through the HRAS/RAF1 pathways, or negative regulation of apoptosis through the PDK1/AKT1 pathway (By similarity). May act in collecting-duct and biliary differentiation (PubMed : 11919560). May participate in the regulation of the cholangiocytes proliferation and the CCN2 production in an CXCL8-dependent manner (PubMed : 30898581).
See full target information PKHD1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 16:58-67 PubMed33364434

2020

SLC12A8 plays a key role in bladder cancer progression and EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Shun-Lai Li,Zheng-Feng Li,Qing-Wei Cao,Wen-Zhen Wang
View all publications
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Product promise

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