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AB196689

Anti-PKIB antibody

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(4 Publications)

Rabbit Polyclonal PKIB antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PKIB.

View Alternative Names

PRKACN2, PKIB, cAMP-dependent protein kinase inhibitor beta, PKI-beta

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PKIB antibody (AB196689)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PKIB antibody (AB196689)

Immunofluorescent analysis of MCF7 cells labeling PKIB with ab196689 at 1/100 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKIB antibody (AB196689)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKIB antibody (AB196689)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PKIB with ab196689 at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human PKIB. The exact immunogen used to generate this antibody is proprietary information.

Q9C010

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab196689 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PKIB or Protein Kinase Inhibitor Beta acts as an inhibitor for protein kinase A (PKA). It belongs to the family of PKI proteins that include PKIA and PKIG. PKIB contributes to the cellular process by binding to PKA and preventing its activity therefore affecting its downstream effects. PKIB has a molecular mass of approximately 9 kDa. It expresses predominantly in tissues such as the prostate and brain reflecting its specific roles in different cellular contexts.
Biological function summary

The inhibition of PKA by PKIB plays a role in regulating cellular proliferation and apoptosis. PKIB does not form part of a larger complex but functions through direct interactions with its target PKA. PKIB's inhibitory action on PKA can modulate several downstream signalling cascades affecting various cellular outcomes according to the cellular context and signals received.

Pathways

PKIB impacts key signalling networks such as the cAMP-dependent PKA pathway and the MAPK/ERK pathway. PKIB's interaction with PKA affects the transduction of signals initiated by cyclic AMP which influences cellular processes diversity. PKIB assists in the modulation of MAPK/ERK pathway working in concert with other proteins like AKAPs that organize PKA signaling compartments allowing for refined control over cellular responses to signaling.

PKIB emerges as relevant in prostate cancer and certain neurological disorders. The association of PKIB with prostate cancer arises from the deregulation of PKA activity affecting cell proliferation and survival. Through interactions with other signaling proteins like CREB dysregulated PKIB expression might contribute to the pathology of cancer. Furthermore altered PKIB expression patterns have also been observed in neurological disorders where imbalanced kinase activities disrupt normal brain function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.
See full target information PKIB
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 14:e70769 PubMed40095726

2025

Exploring Mechanisms and Biomarkers of Breast Cancer Invasion and Migration: An Explainable Gene-Pathway-Compounds Neural Network.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Qian,Dandan Sun,Yichen Ma,Ling Qiu,Jie Wu

Protein & cell 15:530-546 PubMed38486356

2024

Comprehensive transcriptional atlas of human adenomyosis deciphered by the integration of single-cell RNA-sequencing and spatial transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Chen,Yiliang Xu,Xiaocui Xu,Jianzhang Wang,Zhiruo Qiu,Yayuan Yu,Xiaohong Jiang,Wanqi Shao,Dandan Bai,Mingzhu Wang,Shuyan Mei,Tao Cheng,Li Wu,Shaorong Gao,Xuan Che

IUBMB life 72:1468-1480 PubMed32187820

2020

microRNA-495 reduces visceral sensitivity in mice with diarrhea-predominant irritable bowel syndrome through suppression of the PI3K/AKT signaling pathway via PKIB.

Applications

Unspecified application

Species

Unspecified reactive species

Lifeng Fei,Yanjing Wang

Molecular medicine reports 19:2413-2420 PubMed30664211

2019

Expression of S100A8 is induced by interleukin‑1α in TR146 epithelial cells through a mechanism involving CCAAT/enhancer binding protein β.

Applications

Unspecified application

Species

Unspecified reactive species

Mingqun Qin,Yantao Zou,Kanghua Zhong,Yong Guo,Xianqiong Zou
View all publications
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