AB233521

Anti-PKIB antibody

Name: Anti-PKIB antibody (ab233521) | Abcam
Brand: Abcam Limited
SKU: AB233521
Price: 510 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal PKIB antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PKIB aa 1 to C-terminus.

View Alternative Names

PRKACN2, PKIB, cAMP-dependent protein kinase inhibitor beta, PKI-beta

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKIB antibody (AB233521)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKIB antibody (AB233521)

Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for PKIB using ab233521 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKIB antibody (AB233521)

Formalin-fixed, paraffin-embedded human kidney tissue stained for PKIB using ab233521 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKIB antibody (AB233521)

Formalin-fixed, paraffin-embedded human pancreatic cancer tissue stained for PKIB using ab233521 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKIB antibody (AB233521)

Formalin-fixed, paraffin-embedded human prostate tissue stained for PKIB using ab233521 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKIB antibody (AB233521)

Formalin-fixed, paraffin-embedded mouse liver tissue stained for PKIB using ab233521 at 10 μg/mL in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKIB antibody (AB233521)

Formalin-fixed, paraffin-embedded mouse kidney tissue stained for PKIB using ab233521 at 10 μg/mL in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKIB antibody (AB233521)

Formalin-fixed, paraffin-embedded mouse brain tissue stained for PKIB using ab233521 at 10 μg/mL in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-PKIB antibody (AB233521)

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Western blot - Anti-PKIB antibody (ab233521) at 3 µg/mL

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Human placenta tissue lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution

Predicted band size: 8 kDa

false

Supplier Data

Western blot - Anti-PKIB antibody (AB233521)

All lanes:

Western blot - Anti-PKIB antibody (ab233521) at 5 µg/mL

All lanes:

Recombinant human PKIB protein

Predicted band size: 8 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PKIB aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9C010

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PKIB or Protein Kinase Inhibitor Beta acts as an inhibitor for protein kinase A (PKA). It belongs to the family of PKI proteins that include PKIA and PKIG. PKIB contributes to the cellular process by binding to PKA and preventing its activity therefore affecting its downstream effects. PKIB has a molecular mass of approximately 9 kDa. It expresses predominantly in tissues such as the prostate and brain reflecting its specific roles in different cellular contexts.

Biological function summary

The inhibition of PKA by PKIB plays a role in regulating cellular proliferation and apoptosis. PKIB does not form part of a larger complex but functions through direct interactions with its target PKA. PKIB's inhibitory action on PKA can modulate several downstream signalling cascades affecting various cellular outcomes according to the cellular context and signals received.

Pathways

PKIB impacts key signalling networks such as the cAMP-dependent PKA pathway and the MAPK/ERK pathway. PKIB's interaction with PKA affects the transduction of signals initiated by cyclic AMP which influences cellular processes diversity. PKIB assists in the modulation of MAPK/ERK pathway working in concert with other proteins like AKAPs that organize PKA signaling compartments allowing for refined control over cellular responses to signaling.

PKIB emerges as relevant in prostate cancer and certain neurological disorders. The association of PKIB with prostate cancer arises from the deregulation of PKA activity affecting cell proliferation and survival. Through interactions with other signaling proteins like CREB dysregulated PKIB expression might contribute to the pathology of cancer. Furthermore altered PKIB expression patterns have also been observed in neurological disorders where imbalanced kinase activities disrupt normal brain function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.

See full target information PKIB

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:470 PubMed40593489

2025

PKIB facilitates bladder cancer proliferation and metastasis through mediation of HSP27 phosphorylation by PKA.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong Liu,Xiaoyu Yin,Feng Yuan,Shiqing Li,Fei Xiao,Feng Zhou,Xudong Pan,Yatfaat Ho,Shuo Dong,Duan Xu,Yunqing Ma,Zhengding Cao,Zhe Lei,Yi Sun

Molecular cancer therapeutics 23:552-563 PubMed38030378

2023

Glucocorticoid Receptor (GR) Activation Is Associated with Increased cAMP/PKA Signaling in Castration-Resistant Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lynda Bennett,Praveen Kumar Jaiswal,Ryan V Harkless,Tiha M Long,Ning Gao,Brianna Vandenburg,Phillip Selman,Ishrat Durdana,Ricardo R Lastra,Donald Vander Griend,Remi Adelaiye-Ogala,Russell Z Szmulewitz,Suzanne D Conzen

Frontiers in oncology 12:965838 PubMed36072791

2022

involved in the metastasis and survival of osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rongxue Wan,Gu Yang,Qianzhen Liu,Xiaokang Fu,Zengping Liu,Huilai Miao,Huan Liu,Wenhua Huang

Archives of medical science : AMS 20:267-279 PubMed38414469

2021

may promote proliferation and invasion in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Zhong,Zhi-Xi Li,Ling-Jing Yang,Gang Wu,Bo Xiang,Yu-Lan Wang,Qing Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PKIB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKIB antibody (AB233521)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKIB antibody (AB233521)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKIB antibody (AB233521)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKIB antibody (AB233521)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKIB antibody (AB233521)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKIB antibody (AB233521)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKIB antibody (AB233521)

Western blot - Anti-PKIB antibody (AB233521)

Western blot - Anti-PKIB antibody (AB233521)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise