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AB125697

Anti-PKLR antibody

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(2 Publications)

Rabbit Polyclonal PKLR antibody. Suitable for WB, IHC-P and reacts with Rat, Transfected cell lysate - Human, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PKLR aa 150-550.

View Alternative Names

PK1, PKL, PKLR, Pyruvate kinase PKLR, Pyruvate kinase 1, Pyruvate kinase isozymes L/R, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody (AB125697)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody (AB125697)

ab125697, at 1/250 dilution, staining PKLR in Formalin-fixed, Paraffin-embedded Human breast cancer tissue by Immunohistochemistry.

Western blot - Anti-PKLR antibody (AB125697)
  • WB

Supplier Data

Western blot - Anti-PKLR antibody (AB125697)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-PKLR antibody (ab125697) at 1/1000 dilution

Lane 1:

Jurkat whole cell lysates at 30 µg

Lane 2:

Raji whole cell lysates at 30 µg

Lane 3:

K562 whole cell lysates at 30 µg

Predicted band size: 62 kDa

false

Western blot - Anti-PKLR antibody (AB125697)
  • WB

Supplier Data

Western blot - Anti-PKLR antibody (AB125697)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-PKLR antibody (ab125697) at 1/10000 dilution

All lanes:

Rat liver lysate at 20 µg

Predicted band size: 62 kDa

false

Western blot - Anti-PKLR antibody (AB125697)
  • WB

Supplier Data

Western blot - Anti-PKLR antibody (AB125697)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-PKLR antibody (ab125697) at 1/1000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

PKLR transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 62 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PKLR aa 150-550. The exact immunogen used to generate this antibody is proprietary information.

P30613

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perfrom antigen retrieval using heat mediated 10mM Citrate buffer(pH6.0) or Tris-EDTA buffer(pH8.0).</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PKLR gene encodes the pyruvate kinase liver and red blood cell enzyme also known as PK-LR. This enzyme catalyzes the last step in the glycolytic pathway converting phosphoenolpyruvate (PEP) into pyruvate with the production of ATP. The alternative names for PKLR include R-type pyruvate kinase and L-type pyruvate kinase. The enzyme has a molecular mass of approximately 57 kDa. It is primarily expressed in the liver and red blood cells with some presence in the pancreatic islets supporting its physiological roles in these tissues.
Biological function summary

Pyruvate kinase is important for energy production by regulating glycolysis. It exists as a homo-tetramer forming a complex to execute its enzymatic activity. This complex ensures the efficient conversion of PEP facilitating the flow of carbon through the glycolytic pathway. In the liver PKLR plays a role in gluconeogenesis and lipogenesis while in red blood cells it contributes directly to ATP production essential for maintaining cellular energy and function.

Pathways

The pyruvate kinase enzyme is a significant participant in glycolysis and gluconeogenesis. These pathways are central to energy metabolism linking glucose breakdown with ATP generation. Pyruvate kinase interacts with enzymes like hexokinase and phosphofructokinase within the glycolytic pathway coordinating with these enzymes to regulate energy metabolism. In gluconeogenesis PKLR integrates into the mechanism ensuring the balance between energy production and storage.

PKLR mutations can lead to pyruvate kinase deficiency a condition that causes hemolytic anemia. This disorder results from impaired ATP production in red blood cells causing them to break down prematurely. In liver tissues dysregulation of PKLR activity associates with cancers like hepatocellular carcinoma. This enzyme connects with proteins such as lactate dehydrogenase in cancer metabolism affecting the Warburg effect which is tangled with cancer cell survival and proliferation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pyruvate kinase that catalyzes the conversion of phosphoenolpyruvate to pyruvate with the synthesis of ATP, and which plays a key role in glycolysis.
See full target information PKLR
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:252 PubMed35306527

2022

Pyruvate kinase L/R links metabolism dysfunction to neuroendocrine differentiation of prostate cancer by ZBTB10 deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Ching Wen,Wei-Yu Chen,Van Thi Ngoc Tram,Hsiu-Lien Yeh,Wei-Hao Chen,Kuo-Ching Jiang,Wassim Abou-Kheir,Jiaoti Huang,Michael Hsiao,Yen-Nien Liu

Mediators of inflammation 2019:8436985 PubMed31582904

2019

Pregnancy-Associated Plasma Protein A Induces Inflammatory Cytokine Expression by Activating IGF-I/PI3K/Akt Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Weiping Li,Hongwei Li,Li Zhou,Zijian Wang,Bing Hua
View all publications
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Product promise

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