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AB137787

Anti-PKLR antibody

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(3 Publications)

Rabbit Polyclonal PKLR antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PKLR aa 250 to C-terminus.

View Alternative Names

PK1, PKL, PKLR, Pyruvate kinase PKLR, Pyruvate kinase 1, Pyruvate kinase isozymes L/R, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase

3 Images
Western blot - Anti-PKLR antibody (AB137787)
  • WB

Unknown

Western blot - Anti-PKLR antibody (AB137787)

7.5% SDS PAGE

All lanes:

Western blot - Anti-PKLR antibody (ab137787) at 1/1000 dilution

Lane 1:

Jurkat cell extract at 30 µg

Lane 2:

Raji cell extract at 30 µg

Lane 3:

K562 cell extract at 30 µg

Lane 4:

THP-1 cell extract at 30 µg

Lane 5:

NCI-H929 cell extract at 30 µg

Predicted band size: 62 kDa

false

Western blot - Anti-PKLR antibody (AB137787)
  • WB

Unknown

Western blot - Anti-PKLR antibody (AB137787)

7.5% SDS PAGE

All lanes:

Western blot - Anti-PKLR antibody (ab137787) at 1/500 dilution

All lanes:

Rat liver extract at 50 µg

Predicted band size: 62 kDa

false

Western blot - Anti-PKLR antibody (AB137787)
  • WB

Unknown

Western blot - Anti-PKLR antibody (AB137787)

7.5% SDS PAGE

All lanes:

Western blot - Anti-PKLR antibody (ab137787) at 1/500 dilution

All lanes:

Mouse liver extract at 50 µg

Predicted band size: 62 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human PKLR aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P30613

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Reagents, Controls & Accessories

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PKLR gene encodes the pyruvate kinase liver and red blood cell enzyme also known as PK-LR. This enzyme catalyzes the last step in the glycolytic pathway converting phosphoenolpyruvate (PEP) into pyruvate with the production of ATP. The alternative names for PKLR include R-type pyruvate kinase and L-type pyruvate kinase. The enzyme has a molecular mass of approximately 57 kDa. It is primarily expressed in the liver and red blood cells with some presence in the pancreatic islets supporting its physiological roles in these tissues.
Biological function summary

Pyruvate kinase is important for energy production by regulating glycolysis. It exists as a homo-tetramer forming a complex to execute its enzymatic activity. This complex ensures the efficient conversion of PEP facilitating the flow of carbon through the glycolytic pathway. In the liver PKLR plays a role in gluconeogenesis and lipogenesis while in red blood cells it contributes directly to ATP production essential for maintaining cellular energy and function.

Pathways

The pyruvate kinase enzyme is a significant participant in glycolysis and gluconeogenesis. These pathways are central to energy metabolism linking glucose breakdown with ATP generation. Pyruvate kinase interacts with enzymes like hexokinase and phosphofructokinase within the glycolytic pathway coordinating with these enzymes to regulate energy metabolism. In gluconeogenesis PKLR integrates into the mechanism ensuring the balance between energy production and storage.

PKLR mutations can lead to pyruvate kinase deficiency a condition that causes hemolytic anemia. This disorder results from impaired ATP production in red blood cells causing them to break down prematurely. In liver tissues dysregulation of PKLR activity associates with cancers like hepatocellular carcinoma. This enzyme connects with proteins such as lactate dehydrogenase in cancer metabolism affecting the Warburg effect which is tangled with cancer cell survival and proliferation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pyruvate kinase that catalyzes the conversion of phosphoenolpyruvate to pyruvate with the synthesis of ATP, and which plays a key role in glycolysis.
See full target information PKLR
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Diabetology & metabolic syndrome 17:362 PubMed40883798

2025

Activation of M1 macrophages promotes diabetic kidney disease by modulating glycolysis via HIF-1α-HK2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Pei,Lijuan Lu,Ziqiong Huang,Yu Wei,Yu Li,Qiong Wang,Yanli Yang,Langen Zhuang,Guoxi Jin

Cell death & disease 11:355 PubMed32393782

2020

Role of pyruvate kinase M2-mediated metabolic reprogramming during podocyte differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Yuan,Jiao Miao,Qianqian Yang,Li Fang,Yi Fang,Hao Ding,Yang Zhou,Lei Jiang,Chunsun Dai,Ke Zen,Qi Sun,Junwei Yang

Cell death & disease 5:e1212 PubMed24810048

2014

Chaetocin-induced ROS-mediated apoptosis involves ATM-YAP1 axis and JNK-dependent inhibition of glucose metabolism.

Applications

WB

Species

Human

D Dixit,R Ghildiyal,N P Anto,E Sen
View all publications
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Product promise

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