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Rabbit Recombinant Monoclonal PKLR antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (AB171744), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (AB171744), expandable thumbnail
  • Western blot - Anti-PKLR antibody [EPR11093] (AB171744), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (AB171744), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (AB171744), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Not recommended
Not recommended
Tested
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended
Expected
Not recommended

Tested
Tested

Species

Human

Dilution info

1/250 - 1/500

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/250

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/250

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/5000

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Plays a key role in glycolysis.

Alternative names

Pyruvate kinase PKLR, Pyruvate kinase 1, Pyruvate kinase isozymes L/R, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase, PKL, PK1, PKLR

Recommended products

Rabbit Recombinant Monoclonal PKLR antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR11093

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The PKLR gene encodes the pyruvate kinase liver and red blood cell enzyme also known as PK-LR. This enzyme catalyzes the last step in the glycolytic pathway converting phosphoenolpyruvate (PEP) into pyruvate with the production of ATP. The alternative names for PKLR include R-type pyruvate kinase and L-type pyruvate kinase. The enzyme has a molecular mass of approximately 57 kDa. It is primarily expressed in the liver and red blood cells with some presence in the pancreatic islets supporting its physiological roles in these tissues.

Biological function summary

Pyruvate kinase is important for energy production by regulating glycolysis. It exists as a homo-tetramer forming a complex to execute its enzymatic activity. This complex ensures the efficient conversion of PEP facilitating the flow of carbon through the glycolytic pathway. In the liver PKLR plays a role in gluconeogenesis and lipogenesis while in red blood cells it contributes directly to ATP production essential for maintaining cellular energy and function.

Pathways

The pyruvate kinase enzyme is a significant participant in glycolysis and gluconeogenesis. These pathways are central to energy metabolism linking glucose breakdown with ATP generation. Pyruvate kinase interacts with enzymes like hexokinase and phosphofructokinase within the glycolytic pathway coordinating with these enzymes to regulate energy metabolism. In gluconeogenesis PKLR integrates into the mechanism ensuring the balance between energy production and storage.

Associated diseases and disorders

PKLR mutations can lead to pyruvate kinase deficiency a condition that causes hemolytic anemia. This disorder results from impaired ATP production in red blood cells causing them to break down prematurely. In liver tissues dysregulation of PKLR activity associates with cancers like hepatocellular carcinoma. This enzyme connects with proteins such as lactate dehydrogenase in cancer metabolism affecting the Warburg effect which is tangled with cancer cell survival and proliferation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (ab171744), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (ab171744)

    Immunohistochemistry of paraffin-embedded Human liver tissue labeling PKLR using ab171744 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (ab171744), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (ab171744)

    Immunohistochemistry of paraffin-embedded Human kidney tissue labeling PKLR using ab171744 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-PKLR antibody [EPR11093] (ab171744), expandable thumbnail

    Western blot - Anti-PKLR antibody [EPR11093] (ab171744)

    All lanes: Western blot - Anti-PKLR antibody [EPR11093] (ab171744) at 1/1000 dilution

    Lane 1: HT-29 cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: HepG2 cell lysate at 10 µg

    Lane 4: 293T cell lysate at 10 µg

    Predicted band size: 62 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (ab171744), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (ab171744)

    Immunohistochemistry analysis of paraffin-embedded mouse skeletal muscle tissue sections labeling PKLR with ab171744 at 1/250 dilution, incubated for 30 mins at room temperature followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Negative control: no staining on mouse skeletal muscle.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (ab171744), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (ab171744)

    Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue sections labeling PKLR with ab171744 at 1/250 dilution, incubated for 30 mins at room temperature followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Negative control: no staining on human skeletal muscle.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (ab171744), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (ab171744)

    Immunohistochemistry analysis of paraffin-embedded rat liver tissue sections labeling PKLR with ab171744 at 1/250 dilution, incubated for 30 mins at room temperature followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (ab171744), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (ab171744)

    Immunohistochemistry analysis of paraffin-embedded mouse liver tissue sections labeling PKLR with ab171744 at 1/250 dilution, incubated for 30 mins at room temperature followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (ab171744), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (ab171744)

    Immunohistochemistry analysis of paraffin-embedded human liver tissue sections labeling PKLR with ab171744 at 1/250 dilution, incubated for 30 mins at room temperature followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (ab171744), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] (ab171744)

    Immunohistochemistry analysis of paraffin-embedded rat cardiac muscle tissue sections labeling PKLR with ab171744 at 1/250 dilution, incubated for 30 mins at room temperature followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Negative control: no staining on rat cardiac muscle.

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Product protocols

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