Anti-PKLR antibody [EPR11093] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] - BSA and Azide free (AB249648)

This data was developed using ab171744, the same antibody clone in a different buffer formulation.

Immunohistochemistry of paraffin-embedded Human liver tissue labeling PKLR using ab171744 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] - BSA and Azide free (AB249648)

This data was developed using ab171744, the same antibody clone in a different buffer formulation.

Immunohistochemistry of paraffin-embedded Human kidney tissue labeling PKLR using ab171744 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] - BSA and Azide free (AB249648)

This data was developed using ab171744, the same antibody clone in a different buffer formulation. Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue sections labeling PKLR with ab171744 at 1/250 dilution, incubated for 30 mins at room temperature followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Negative control : no staining on human skeletal muscle.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] - BSA and Azide free (AB249648)

This data was developed using ab171744, the same antibody clone in a different buffer formulation. Immunohistochemistry analysis of paraffin-embedded human liver tissue sections labeling PKLR with ab171744 at 1/250 dilution, incubated for 30 mins at room temperature followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] - BSA and Azide free (AB249648)

This data was developed using ab171744, the same antibody clone in a different buffer formulation. Immunohistochemistry analysis of paraffin-embedded rat cardiac muscle tissue sections labeling PKLR with ab171744 at 1/250 dilution, incubated for 30 mins at room temperature followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Negative control : no staining on rat cardiac muscle.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] - BSA and Azide free (AB249648)

This data was developed using ab171744, the same antibody clone in a different buffer formulation. Immunohistochemistry analysis of paraffin-embedded mouse skeletal muscle tissue sections labeling PKLR with ab171744 at 1/250 dilution, incubated for 30 mins at room temperature followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Negative control : no staining on mouse skeletal muscle.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] - BSA and Azide free (AB249648)

This data was developed using ab171744, the same antibody clone in a different buffer formulation. Immunohistochemistry analysis of paraffin-embedded rat liver tissue sections labeling PKLR with ab171744 at 1/250 dilution, incubated for 30 mins at room temperature followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKLR antibody [EPR11093] - BSA and Azide free (AB249648)

This data was developed using ab171744, the same antibody clone in a different buffer formulation. Immunohistochemistry analysis of paraffin-embedded mouse liver tissue sections labeling PKLR with ab171744 at 1/250 dilution, incubated for 30 mins at room temperature followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Sections were counterstained with Hematoxylin. Antigen retrieval  was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• WB

Supplier Data

Western blot - Anti-PKLR antibody [EPR11093] - BSA and Azide free (AB249648)

This data was developed using ab171744, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PKLR antibody [EPR11093] (<a href='/en-us/products/primary-antibodies/pklr-antibody-epr11093-ab171744'>ab171744</a>) at 1/1000 dilution

Lane 1:

HT-29 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Predicted band size: 62 kDa

false