Anti-PKM antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PKM antibody (AB137791)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling PKM protein at cytoplasm using ab137791 at a 1/500 dilution (Green). DAPI was used for nuclear staining.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody (AB137791)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue staining PKM protein at cytosol with ab137791 at 1/500 dilution. Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

• IP

Supplier Data

Immunoprecipitation - Anti-PKM antibody (AB137791)

Immunoprecipitation of PKM protein from 293T whole cell extracts using 5 µg of PKM antibody.
Western blot analysis was performed using PKM antibody.

All lanes:

Immunoprecipitation - Anti-PKM antibody (ab137791) at 5 µg

Lane 1:

293T whole cell extracts (input)

Lane 2:

293T whole cell extracts with control (-)

Lane 3:

293T whole cell extracts (+)

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Observed band size: 55 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-PKM antibody (AB137791)

Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell labelling Pyruvate Kinase (muscle) antibody detects Pyruvate Kinase (muscle) protein at cytoplasm (green) with ab137791 at 1/500 dilution. Red : alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/500 dilution. Blue : Hoechst 33342 staining.

All lanes:

Immunoprecipitation - Anti-PKM antibody (ab137791)

Predicted band size: 58 kDa

false

• WB

Supplier Data

Western blot - Anti-PKM antibody (AB137791)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-PKM antibody (ab137791) at 1/5000 dilution

Lane 1:

Non-transfected (-) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

HEK-293T whole cell extracts transfected (+) with PKM shRNA at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 58 kDa

false

• WB

Supplier Data

Western blot - Anti-PKM antibody (AB137791)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-PKM antibody (ab137791) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 58 kDa

false

• WB

Supplier Data

Western blot - Anti-PKM antibody (AB137791)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PKM antibody (ab137791) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 55 kDa

false

• WB

Supplier Data

Western blot - Anti-PKM antibody (AB137791)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PKM antibody (ab137791) at 1/3000 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Rat brain tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 55 kDa

false

• WB

Supplier Data

Western blot - Anti-PKM antibody (AB137791)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-PKM antibody (ab137791) at 1/5000 dilution

Lane 1:

Non-transfected (–) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

Transfected (+) HEK-293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 58 kDa

false