Rabbit Polyclonal KPYM antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat, Transfected cell lysate samples. Cited in 42 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PKM aa 1-250.
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- International journal of oncology 63:2023USP35 promotes hepatocellular carcinoma progression by protecting PKM2 from ubiquitination‑mediated degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Lv,Bo Zhang,Chenghao Jiang,Qiwen Zeng,Jiayin Yang,Yongjie ZhouPubMed 37594129
- Cell communication and signaling : CCS 20:1942022Docetaxel suppressed cell proliferation through Smad3/HIF-1α-mediated glycolysis in prostate cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunming Peng,Zhijun He,Yeqing Yuan,Jing Xie,Yu Zhou,Baochun Guo,Jinan GuoPubMed 36536346
- International journal of molecular sciences 23:2022Leucine and Arginine Availability Modulate Mouse Embryonic Stem Cell Proliferation and Metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBibiana Correia,Maria Inês Sousa,Ana Filipa Branco,Ana Sofia Rodrigues,João Ramalho-SantosPubMed 36430764
- Cell cycle (Georgetown, Tex.) 22:680-6922022miR-140-3p/usp36 axis mediates ubiquitination to regulate PKM2 and suppressed the malignant biological behavior of breast cancer through Warburg effect.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Wu,Yile Jiao,Chen Zhou,Xinyi Guo,Zhenru Wu,Qing LvPubMed 36305548
- Cellular & molecular biology letters 27:922022Targeting UBR5 in hepatocellular carcinoma cells and precise treatment via echinacoside nanodelivery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMenghan Wang,Xing Ma,Guoyu Wang,Yanan Song,Miao Zhang,Zhongchao Mai,Borong Zhou,Ying Ye,Wei XiaPubMed 36224534
- Frontiers in oncology 12:9280222022lncRNA NEAT1 promotes the proliferation and metastasis of hepatocellular carcinoma by regulating the FOXP3/PKM2 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunping Pan,Yingzhe Hu,Chenlu Yuan,Yafu Wu,Xinhua ZhuPubMed 36185217
- Journal of orthopaedic surgery and research 17:4212022Circ_0136666 aggravates osteosarcoma development through mediating miR-1244/CEP55 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Gao,Nanwei Xu,Kaisong Miao,Gao Huang,Yong HuangPubMed 36109749
- International journal of molecular sciences 23:2022Metformin Mitigated Obesity-Driven Cancer Aggressiveness in Tumor-Bearing Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Jung Chen,Chih-Cheng Wu,Cheng-Yi Chang,Jian-Ri Li,Yen-Chuan Ou,Wen-Ying Chen,Su-Lan Liao,Jiaan-Der WangPubMed 36012397
- Cell death & disease 13:4632022m6A methylation mediates LHPP acetylation as a tumour aerobic glycolysis suppressor to improve the prognosis of gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian-Xian Lin,Ning-Zi Lian,You-Xin Gao,Qiao-Ling Zheng,Ying-Hong Yang,Yu-Bin Ma,Zhi-Song Xiu,Qing-Zhu Qiu,Hua-Gen Wang,Chao-Hui Zheng,Ping Li,Jian-Wei Xie,Jun Lu,Qi-Yue Chen,Long-Long Cao,Mi Lin,Jia-Bin Wang,Chang-Ming HuangPubMed 35568711
- Journal of clinical laboratory analysis 36:e243482022CircRNA hsa_circ_0018289 exerts an oncogenic role in cervical cancer progression through miR-1294/ICMT axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYujuan Li,Xiangrong Gao,Chaochao Yang,Hua Yan,Cui LiPubMed 35312113
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PKM aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P14618
Consider this alternative
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
| Transfected cell lysate | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Please see protocol details in the legend for IHC-P on human lung tissue. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes - |
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Target data
Function
Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP (PubMed:15996096, PubMed:1854723, PubMed:20847263). The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production (PubMed:15996096, PubMed:1854723, PubMed:20847263). The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival (PubMed:15996096, PubMed:1854723, PubMed:20847263). Isoform M2. Isoform specifically expressed during embryogenesis that has low pyruvate kinase activity by itself and requires allosteric activation by D-fructose 1,6-bisphosphate (FBP) for pyruvate kinase activity (PubMed:18337823, PubMed:20847263). In addition to its pyruvate kinase activity in the cytoplasm, also acts as a regulator of transcription in the nucleus by acting as a protein kinase (PubMed:18191611, PubMed:21620138, PubMed:22056988, PubMed:22306293, PubMed:22901803, PubMed:24120661). Translocates into the nucleus in response to various signals, such as EGF receptor activation, and homodimerizes, leading to its conversion into a protein threonine- and tyrosine-protein kinase (PubMed:22056988, PubMed:22306293, PubMed:22901803, PubMed:24120661, PubMed:26787900). Catalyzes phosphorylation of STAT3 at 'Tyr-705' and histone H3 at 'Thr-11' (H3T11ph), leading to activate transcription (PubMed:22306293, PubMed:22901803, PubMed:24120661). Its ability to activate transcription plays a role in cancer cells by promoting cell proliferation and promote tumorigenesis (PubMed:18337823, PubMed:22901803, PubMed:26787900). Promotes the expression of the immune checkpoint protein CD274 in BMAL1-deficient macrophages (By similarity). May also act as a translation regulator for a subset of mRNAs, independently of its pyruvate kinase activity: associates with subpools of endoplasmic reticulum-associated ribosomes, binds directly to the mRNAs translated at the endoplasmic reticulum and promotes translation of these endoplasmic reticulum-destined mRNAs (By similarity). Plays a role in caspase independent cell death of tumor cells (PubMed:17308100). Isoform M1. Pyruvate kinase isoform expressed in adult tissues, which replaces isoform M2 after birth (PubMed:18337823). In contrast to isoform M2, has high pyruvate kinase activity by itself and does not require allosteric activation by D-fructose 1,6-bisphosphate (FBP) for activity (PubMed:20847263).
Alternative names
OIP3, PK2, PK3, PKM2, PKM, Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, Opa-interacting protein 3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Threonine-protein kinase PKM2, Thyroid hormone-binding protein 1, Tumor M2-PK, Tyrosine-protein kinase PKM2, p58, CTHBP, OIP-3, THBP1
Recommended products
Rabbit Polyclonal KPYM antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat, Transfected cell lysate samples. Cited in 42 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PKM aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PKM aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P14618
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PKM also known as pyruvate kinase muscle isozyme (PKM) and PEP is an enzyme that plays an important role in glycolysis by catalysing the conversion of phosphoenolpyruvate (PEP) to pyruvate yielding ATP in the process. The PKM protein has two isoforms PKM1 and PKM2 which result from alternative splicing of the PKM gene. The mass of PKM2 the more studied isoform is approximately 58 kDa. PKM is expressed in various tissues prominently in skeletal muscle heart brain and many tumor cells. Additionally PKM has significant activity in rapidly proliferating cells suggesting its importance in high-energy demanding environments.
- Biological function summary
PKM functions not only in catalyzing the last step of glycolysis but also regulates metabolic and transcriptional processes. Specifically PKM2 is a participant in the regulation of gene expression and cellular response to oxidative stress and nutrient availability. It can exist as a dimer or tetramer with the latter being the more active form in glycolytic pathways while the dimeric form can translocate to the nucleus to perform functions unrelated to its glycolytic activity. These transformations make PKM part of a dynamic complex that responds to various cellular signals.
- Pathways
PKM integrates into essential metabolic pathways including the glycolytic pathway and influences the pentose phosphate pathway. It works in conjunction with phosphofructokinase-1 (PFK1) another key glycolytic enzyme synchronizing the energy production process in cells. PKM2's non-metabolic roles involve interactions in signaling pathways related to cellular proliferation and survival often interacting with and modulating proteins like HIF-1α which plays a central role in cellular responses to hypoxia.
- Associated diseases and disorders
PKM2 shows strong connections to cancer and metabolic diseases. Tumor cells often exhibit a shift in expression from PKM1 to PKM2 facilitating the altered metabolism known as the Warburg effect characterized by increased aerobic glycolysis. Its interaction with HIF-1α promotes adaptation to low oxygen environments typical in tumorous growth. Furthermore PKM disruptions or aberrations contribute to metabolic disorders such as diabetes where altered glucose metabolism becomes evident. The protein's behavior in these disease conditions indicates potential targets for therapeutic intervention highlighting the importance of PKM in both normal physiology and pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-PKM antibody (ab137852)
7.5% SDS-PAGE.
Running conditions: 80V, 15min; 140V, 40 minutes.
Transfer condition: Semi-dry, 18 V, 60 min (NC membrane).
Blocking condition: 5% non-fat milk in TBST, RT, 60 minutes.
Primary antibody incubation: 4℃ , overnight.
Washing condition: 5 ml TBST, 4 x 5 minutes.
Exposure: chemiluminescent substrate for the detection of HRP-conjugated antibody
All lanes: Western blot - Anti-PKM antibody (ab137852) at 1/500 dilution
Lane 1: Non-transfected (–) 293T whole cell extracts at 30 µg
Lane 2: 293T whole cell extracts transfected (+) with PKM shRNA at 30 µg
SecondaryAll lanes: Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 58 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody (ab137852)
Paraffin-embedded human lung tissue stained for PKM using ab137852 at 1/500 dilution (4°C overnight) in immunohistochemical analysis.
Secondary antibody: ABC HRP Kit (Rabbit IgG) 1/200 at room temperature for 30 minutes.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes. (Cuisinart Electric Pressure Cooker, choose "high pressure").
Endogenous peroxide blocking: 3% H2O2, RT, 30minutes.
Blocking condition: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 minutes.
Washing condition: PBS, 2 x 5 minutes.
Chromogen system: DAB.
Western blot - Anti-PKM antibody (ab137852)
10% SDS-PAGE.
Running conditions: 80V, 15min; 140V, 40 minutes.
Transfer condition: Semi-dry, 18 V, 60 min (NC membrane).
Blocking condition: 5% non-fat milk in TBST, RT, 60 minutes.
Primary antibody incubation: 4℃ , overnight.
Washing condition: 5 ml TBST, 4 x 5 minutes.
Exposure: enhanced ECL
All lanes: Western blot - Anti-PKM antibody (ab137852) at 1/1000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Lane 3: H1299 whole cell lysate at 30 µg
Lane 4: HeLa S3 whole cell lysate at 30 µg
Lane 5: Hep G2 whole cell lysate at 30 µg
Lane 6: MOLT4 whole cell lysate at 30 µg
Lane 7: Raji whole cell lysate at 30 µg
SecondaryAll lanes: Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 58 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody (ab137852)
Immunohistochemical analysis of paraffin-embedded PKM DLD1 xenograft tissue labelling PKM with ab137852 at 1/100 dilution.
Western blot - Anti-PKM antibody (ab137852)
7.5% SDS PAGE
All lanes: Western blot - Anti-PKM antibody (ab137852) at 1/1000 dilution
All lanes: Mouse brain whole cell lysate at 50 µg
Predicted band size: 58 kDa
Western blot - Anti-PKM antibody (ab137852)
7.5 % SDS-PAGE
All lanes: Western blot - Anti-PKM antibody (ab137852) at 1/1000 dilution
All lanes: Rat brain lysate/extract at 50 µg
Predicted band size: 58 kDa, 60 kDa
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