AB137852

Anti-PKM antibody

Name: Anti-PKM antibody (ab137852) | Abcam
Brand: Abcam Limited
SKU: AB137852
Rating: 4 (2 reviews)

(2 Reviews)

(56 Publications)

Rabbit Polyclonal KPYM antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat, Transfected cell lysate samples. Cited in 56 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PKM aa 1-250.

View Alternative Names

OIP3, PK2, PK3, PKM2, PKM, Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, Opa-interacting protein 3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Threonine-protein kinase PKM2, Thyroid hormone-binding protein 1, Tumor M2-PK, Tyrosine-protein kinase PKM2, p58, CTHBP, OIP-3, THBP1

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-PKM antibody (AB137852)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PKM antibody (AB137852)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling PKM protein at cytoplasm using ab137852 at a 1/500 dilution (Green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody (AB137852)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody (AB137852)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue stained for PKM protein at cytoplasm with ab137852 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-PKM antibody (AB137852)

7.5% SDS-PAGE.

Running conditions : 80V, 15min; 140V, 40 minutes.

Transfer condition : Semi-dry, 18 V, 60 min (NC membrane).

Blocking condition : 5% non-fat milk in TBST, RT, 60 minutes.

Primary antibody incubation : 4℃ , overnight.

Washing condition : 5 ml TBST, 4 x 5 minutes.

Exposure : chemiluminescent substrate for the detection of HRP-conjugated antibody

All lanes:

Western blot - Anti-PKM antibody (ab137852) at 1/500 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

293T whole cell extracts transfected (+) with PKM shRNA at 30 µg

Secondary

All lanes:

Rabbit IgG antibody (HRP) at 1/10000 dilution

Predicted band size: 58 kDa

false

Supplier Data

Western blot - Anti-PKM antibody (AB137852)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PKM antibody (ab137852) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 58 kDa

false

Supplier Data

Western blot - Anti-PKM antibody (AB137852)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-PKM antibody (ab137852) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 58 kDa

false

Unknown

Western blot - Anti-PKM antibody (AB137852)

7.5 % SDS-PAGE

All lanes:

Western blot - Anti-PKM antibody (ab137852) at 1/1000 dilution

All lanes:

Rat brain lysate/extract at 50 µg

Predicted band size: 58 kDa,60 kDa

false

Supplier Data

Western blot - Anti-PKM antibody (AB137852)

Samples were separated by 10% SDS-PAGE. The signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-PKM antibody (ab137852) at 1/1000 dilution

All lanes:

Mouse brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 58 kDa

true

Supplier Data

Western blot - Anti-PKM antibody (AB137852)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-PKM antibody (ab137852) at 1/500 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

Transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PKM aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P14618

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Please see protocol details in the legend for IHC-P on human lung tissue.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PKM also known as pyruvate kinase muscle isozyme (PKM) and PEP is an enzyme that plays an important role in glycolysis by catalysing the conversion of phosphoenolpyruvate (PEP) to pyruvate yielding ATP in the process. The PKM protein has two isoforms PKM1 and PKM2 which result from alternative splicing of the PKM gene. The mass of PKM2 the more studied isoform is approximately 58 kDa. PKM is expressed in various tissues prominently in skeletal muscle heart brain and many tumor cells. Additionally PKM has significant activity in rapidly proliferating cells suggesting its importance in high-energy demanding environments.

Biological function summary

PKM functions not only in catalyzing the last step of glycolysis but also regulates metabolic and transcriptional processes. Specifically PKM2 is a participant in the regulation of gene expression and cellular response to oxidative stress and nutrient availability. It can exist as a dimer or tetramer with the latter being the more active form in glycolytic pathways while the dimeric form can translocate to the nucleus to perform functions unrelated to its glycolytic activity. These transformations make PKM part of a dynamic complex that responds to various cellular signals.

Pathways

PKM integrates into essential metabolic pathways including the glycolytic pathway and influences the pentose phosphate pathway. It works in conjunction with phosphofructokinase-1 (PFK1) another key glycolytic enzyme synchronizing the energy production process in cells. PKM2's non-metabolic roles involve interactions in signaling pathways related to cellular proliferation and survival often interacting with and modulating proteins like HIF-1α which plays a central role in cellular responses to hypoxia.

PKM2 shows strong connections to cancer and metabolic diseases. Tumor cells often exhibit a shift in expression from PKM1 to PKM2 facilitating the altered metabolism known as the Warburg effect characterized by increased aerobic glycolysis. Its interaction with HIF-1α promotes adaptation to low oxygen environments typical in tumorous growth. Furthermore PKM disruptions or aberrations contribute to metabolic disorders such as diabetes where altered glucose metabolism becomes evident. The protein's behavior in these disease conditions indicates potential targets for therapeutic intervention highlighting the importance of PKM in both normal physiology and pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP (PubMed : 15996096, PubMed : 1854723, PubMed : 20847263). The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production (PubMed : 15996096, PubMed : 1854723, PubMed : 20847263). The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival (PubMed : 15996096, PubMed : 1854723, PubMed : 20847263).. Isoform M2. Isoform specifically expressed during embryogenesis that has low pyruvate kinase activity by itself and requires allosteric activation by D-fructose 1,6-bisphosphate (FBP) for pyruvate kinase activity (PubMed : 18337823, PubMed : 20847263). In addition to its pyruvate kinase activity in the cytoplasm, also acts as a regulator of transcription in the nucleus by acting as a protein kinase (PubMed : 18191611, PubMed : 21620138, PubMed : 22056988, PubMed : 22306293, PubMed : 22901803, PubMed : 24120661). Translocates into the nucleus in response to various signals, such as EGF receptor activation, and homodimerizes, leading to its conversion into a protein threonine- and tyrosine-protein kinase (PubMed : 22056988, PubMed : 22306293, PubMed : 22901803, PubMed : 24120661, PubMed : 26787900). Catalyzes phosphorylation of STAT3 at 'Tyr-705' and histone H3 at 'Thr-11' (H3T11ph), leading to activate transcription (PubMed : 22306293, PubMed : 22901803, PubMed : 24120661). Its ability to activate transcription plays a role in cancer cells by promoting cell proliferation and promote tumorigenesis (PubMed : 18337823, PubMed : 22901803, PubMed : 26787900). Promotes the expression of the immune checkpoint protein CD274 in BMAL1-deficient macrophages (By similarity). May also act as a translation regulator for a subset of mRNAs, independently of its pyruvate kinase activity : associates with subpools of endoplasmic reticulum-associated ribosomes, binds directly to the mRNAs translated at the endoplasmic reticulum and promotes translation of these endoplasmic reticulum-destined mRNAs (By similarity). Plays a role in caspase independent cell death of tumor cells (PubMed : 17308100).. Isoform M1. Pyruvate kinase isoform expressed in adult tissues, which replaces isoform M2 after birth (PubMed : 18337823). In contrast to isoform M2, has high pyruvate kinase activity by itself and does not require allosteric activation by D-fructose 1,6-bisphosphate (FBP) for activity (PubMed : 20847263).

See full target information PKM

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Publications (56)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 115:3305-3319 PubMed39073190

2024

Blocking CXCR4-CARM1-YAP axis overcomes osteosarcoma doxorubicin resistance by suppressing aerobic glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Zihua Li,Hengli Lu,Yiwei Zhang,Jiyang Lv,Yi Zhang,Tianyang Xu,Dong Yang,Zhengwei Duan,Yonghao Guan,Zongrui Jiang,Kaiyuan Liu,Yuxin Liao

International journal of oncology 63: PubMed37594129

2023

USP35 promotes hepatocellular carcinoma progression by protecting PKM2 from ubiquitination‑mediated degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Lv,Bo Zhang,Chenghao Jiang,Qiwen Zeng,Jiayin Yang,Yongjie Zhou

Environmental toxicology 38:1332-1346 PubMed36880413

2023

Exosomal ZFPM2-AS1 contributes to tumorigenesis, metastasis, stemness, macrophage polarization, and infiltration in hepatocellular carcinoma through PKM mediated glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Ji,Jie Bai,Yue Ke

Cell communication and signaling : CCS 20:194 PubMed36536346

2022

Docetaxel suppressed cell proliferation through Smad3/HIF-1α-mediated glycolysis in prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Junming Peng,Zhijun He,Yeqing Yuan,Jing Xie,Yu Zhou,Baochun Guo,Jinan Guo

International journal of molecular sciences 23: PubMed36430764

2022

Leucine and Arginine Availability Modulate Mouse Embryonic Stem Cell Proliferation and Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Bibiana Correia,Maria Inês Sousa,Ana Filipa Branco,Ana Sofia Rodrigues,João Ramalho-Santos

Cell cycle (Georgetown, Tex.) 22:680-692 PubMed36305548

2022

miR-140-3p/usp36 axis mediates ubiquitination to regulate PKM2 and suppressed the malignant biological behavior of breast cancer through Warburg effect.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wu,Yile Jiao,Chen Zhou,Xinyi Guo,Zhenru Wu,Qing Lv

Cellular & molecular biology letters 27:92 PubMed36224534

2022

Targeting UBR5 in hepatocellular carcinoma cells and precise treatment via echinacoside nanodelivery.

Applications

Unspecified application

Species

Unspecified reactive species

Menghan Wang,Xing Ma,Guoyu Wang,Yanan Song,Miao Zhang,Zhongchao Mai,Borong Zhou,Ying Ye,Wei Xia

International journal of oncology 61: PubMed36196894

2022

TRIM35 ubiquitination regulates the expression of PKM2 tetramer and dimer and affects the malignant behaviour of breast cancer by regulating the Warburg effect.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wu,Xinyi Guo,Yile Jiao,Zhenru Wu,Qing Lv

Frontiers in oncology 12:928022 PubMed36185217

2022

lncRNA NEAT1 promotes the proliferation and metastasis of hepatocellular carcinoma by regulating the FOXP3/PKM2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Junping Pan,Yingzhe Hu,Chenlu Yuan,Yafu Wu,Xinhua Zhu

Journal of orthopaedic surgery and research 17:421 PubMed36109749

2022

Circ_0136666 aggravates osteosarcoma development through mediating miR-1244/CEP55 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Gao,Nanwei Xu,Kaisong Miao,Gao Huang,Yong Huang

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Product promise

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Anti-PKM antibody

Immunocytochemistry/ Immunofluorescence - Anti-PKM antibody (AB137852)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKM antibody (AB137852)

Western blot - Anti-PKM antibody (AB137852)

Western blot - Anti-PKM antibody (AB137852)

Western blot - Anti-PKM antibody (AB137852)

Western blot - Anti-PKM antibody (AB137852)

Western blot - Anti-PKM antibody (AB137852)

Western blot - Anti-PKM antibody (AB137852)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (56)

Product promise