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AB206129

Anti-PKM antibody [EPR10138(B)] - BSA and Azide free

5

(2 Reviews)

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(5 Publications)

Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (ab206129) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting PKM in Western Blot, Flow Cytometry (Intra), ICC/IF. Suitable for Human, Mouse, Rat.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

OIP3, PK2, PK3, PKM2, PKM, Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, Opa-interacting protein 3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Threonine-protein kinase PKM2, Thyroid hormone-binding protein 1, Tumor M2-PK, Tyrosine-protein kinase PKM2, p58, CTHBP, OIP-3, THBP1

3 Images
Flow Cytometry (Intracellular) - Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (AB206129)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (AB206129)

This data was developed using ab206129, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling PKM with Purified ab206129 at 1 : 50 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor™ 488, ab150077) secondary antibody was used at 1 : 2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (AB206129)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (AB206129)

This data was developed using ab150377, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of A549(Human lung carcinoma epithelial cell) cells labeling PKM with Purified ab150377 at 1/50 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A+H10 : L10] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Western blot - Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (AB206129)
  • WB

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Western blot - Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (AB206129)

All lanes:

Western blot - Anti-PKM antibody [EPR10138(B)] (<a href='/en-us/products/primary-antibodies/pkm-antibody-epr10138b-ab150377'>ab150377</a>) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 3:

A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Mouse lung lysate at 20 µg

Lane 5:

Human skeletal muscle lysate at 20 µg

Lane 6:

Rat skeletal muscle lysate at 20 µg

Lane 7:

Mouse skeletal muscle lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 58 kDa

Observed band size: 58 kDa

false

  • Unconjugated

    Anti-PKM antibody [EPR10138(B)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PKM antibody [EPR10138(B)]

  • 660 APC

    APC Anti-PKM antibody [EPR10138(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PKM antibody [EPR10138(B)]

  • 578 PE

    PE Anti-PKM antibody [EPR10138(B)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-PKM antibody [EPR10138(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10138(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (ab206129) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of PKM?
Anti-PKM [EPR10138(B)] - BSA and Azide free (ab206129) specifically detects a band for PKM (UniProt: P14618) at a molecular weight of 58kDa.

Other related products
We have a range of other formats of antibody clone [EPR10138(B)] also available for your convenience: ab150377, Carrier free - ab206129, PE - ab210448, Alexa Fluor® 647 - ab214257, APC - ab319294, Alexa Fluor® 488 - ab319571, Alexa Fluor® 594 - ab319787, Alexa Fluor® 555 - ab319928

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PKM also known as pyruvate kinase muscle isozyme (PKM) and PEP is an enzyme that plays an important role in glycolysis by catalysing the conversion of phosphoenolpyruvate (PEP) to pyruvate yielding ATP in the process. The PKM protein has two isoforms PKM1 and PKM2 which result from alternative splicing of the PKM gene. The mass of PKM2 the more studied isoform is approximately 58 kDa. PKM is expressed in various tissues prominently in skeletal muscle heart brain and many tumor cells. Additionally PKM has significant activity in rapidly proliferating cells suggesting its importance in high-energy demanding environments.
Biological function summary

PKM functions not only in catalyzing the last step of glycolysis but also regulates metabolic and transcriptional processes. Specifically PKM2 is a participant in the regulation of gene expression and cellular response to oxidative stress and nutrient availability. It can exist as a dimer or tetramer with the latter being the more active form in glycolytic pathways while the dimeric form can translocate to the nucleus to perform functions unrelated to its glycolytic activity. These transformations make PKM part of a dynamic complex that responds to various cellular signals.

Pathways

PKM integrates into essential metabolic pathways including the glycolytic pathway and influences the pentose phosphate pathway. It works in conjunction with phosphofructokinase-1 (PFK1) another key glycolytic enzyme synchronizing the energy production process in cells. PKM2's non-metabolic roles involve interactions in signaling pathways related to cellular proliferation and survival often interacting with and modulating proteins like HIF-1α which plays a central role in cellular responses to hypoxia.

PKM2 shows strong connections to cancer and metabolic diseases. Tumor cells often exhibit a shift in expression from PKM1 to PKM2 facilitating the altered metabolism known as the Warburg effect characterized by increased aerobic glycolysis. Its interaction with HIF-1α promotes adaptation to low oxygen environments typical in tumorous growth. Furthermore PKM disruptions or aberrations contribute to metabolic disorders such as diabetes where altered glucose metabolism becomes evident. The protein's behavior in these disease conditions indicates potential targets for therapeutic intervention highlighting the importance of PKM in both normal physiology and pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP (PubMed : 15996096, PubMed : 1854723, PubMed : 20847263). The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production (PubMed : 15996096, PubMed : 1854723, PubMed : 20847263). The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival (PubMed : 15996096, PubMed : 1854723, PubMed : 20847263).. Isoform M2. Isoform specifically expressed during embryogenesis that has low pyruvate kinase activity by itself and requires allosteric activation by D-fructose 1,6-bisphosphate (FBP) for pyruvate kinase activity (PubMed : 18337823, PubMed : 20847263). In addition to its pyruvate kinase activity in the cytoplasm, also acts as a regulator of transcription in the nucleus by acting as a protein kinase (PubMed : 18191611, PubMed : 21620138, PubMed : 22056988, PubMed : 22306293, PubMed : 22901803, PubMed : 24120661). Translocates into the nucleus in response to various signals, such as EGF receptor activation, and homodimerizes, leading to its conversion into a protein threonine- and tyrosine-protein kinase (PubMed : 22056988, PubMed : 22306293, PubMed : 22901803, PubMed : 24120661, PubMed : 26787900). Catalyzes phosphorylation of STAT3 at 'Tyr-705' and histone H3 at 'Thr-11' (H3T11ph), leading to activate transcription (PubMed : 22306293, PubMed : 22901803, PubMed : 24120661). Its ability to activate transcription plays a role in cancer cells by promoting cell proliferation and promote tumorigenesis (PubMed : 18337823, PubMed : 22901803, PubMed : 26787900). Promotes the expression of the immune checkpoint protein CD274 in BMAL1-deficient macrophages (By similarity). May also act as a translation regulator for a subset of mRNAs, independently of its pyruvate kinase activity : associates with subpools of endoplasmic reticulum-associated ribosomes, binds directly to the mRNAs translated at the endoplasmic reticulum and promotes translation of these endoplasmic reticulum-destined mRNAs (By similarity). Plays a role in caspase independent cell death of tumor cells (PubMed : 17308100).. Isoform M1. Pyruvate kinase isoform expressed in adult tissues, which replaces isoform M2 after birth (PubMed : 18337823). In contrast to isoform M2, has high pyruvate kinase activity by itself and does not require allosteric activation by D-fructose 1,6-bisphosphate (FBP) for activity (PubMed : 20847263).
See full target information PKM
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature 644:790-798 PubMed40533564

2025

Kupffer cell programming by maternal obesity triggers fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Huang,Nora R Balzer,Lea Seep,Iva Splichalova,Nelli Blank-Stein,Maria Francesca Viola,Eliana Franco Taveras,Kerim Acil,Diana Fink,Franzisca Petrovic,Nikola Makdissi,Seyhmus Bayar,Katharina Mauel,Carolin Radwaniak,Jelena Zurkovic,Amir H Kayvanjoo,Klaus Wunderling,Malin Jessen,Mohamed H Yaghmour,Lukas Kenner,Thomas Ulas,Stephan Grein,Joachim L Schultze,Charlotte L Scott,Martin Guilliams,Zhaoyuan Liu,Florent Ginhoux,Marc D Beyer,Christoph Thiele,Felix Meissner,Jan Hasenauer,Dagmar Wachten,Elvira Mass

The Journal of clinical investigation 134: PubMed38954588

2024

Inhibiting the NADase CD38 improves cytomegalovirus-specific CD8+ T cell functionality and metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Nils Mülling,Felix M Behr,Graham A Heieis,Kristina Boss,Suzanne van Duikeren,Floortje J van Haften,Iris N Pardieck,Esmé Ti van der Gracht,Ward Vleeshouwers,Tetje C van der Sluis,J Fréderique de Graaf,Dominique Mb Veerkamp,Kees Lmc Franken,Xin Lei,Lukas van de Sand,Sjoerd H van der Burg,Marij Jp Welters,Sebastiaan Heidt,Wesley Huisman,Simon P Jochems,Martin Giera,Oliver Witzke,Aiko Pj de Vries,Andreas Kribben,Bart Everts,Benjamin Wilde,Ramon Arens

Nature communications 14:5627 PubMed37699869

2023

Metabolic heterogeneity of tissue-resident macrophages in homeostasis and during helminth infection.

Applications

Flow Cyt (Intra)

Species

Mouse

Graham A Heieis,Thiago A Patente,Luís Almeida,Frank Vrieling,Tamar Tak,Georgia Perona-Wright,Rick M Maizels,Rinke Stienstra,Bart Everts

Cell reports 40:111032 PubMed35793635

2022

mTORC1 signaling in antigen-presenting cells of the skin restrains CD8 T cell priming.

Applications

Unspecified application

Species

Unspecified reactive species

Leonard R Pelgrom,Thiago A Patente,Frank Otto,Lonneke V Nouwen,Arifa Ozir-Fazalalikhan,Alwin J van der Ham,Hendrik J P van der Zande,Graham A Heieis,Ramon Arens,Bart Everts

Developmental biology 385:242-52 PubMed24247007

2013

Bi-directional communication with the cumulus cells is involved in the deficiency of XY oocytes in the components essential for proper second meiotic spindle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Baozeng Xu,Saeid Noohi,Jonghyun S Shin,Seang Lin Tan,Teruko Taketo
View all publications
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