AB200387

Anti-PKMYT1 antibody

Name: Anti-PKMYT1 antibody (ab200387) | Abcam
Brand: Abcam Limited
SKU: AB200387
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal PKMYT1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PKMYT1.

View Alternative Names

MYT1, PKMYT1, Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKMYT1 antibody (AB200387)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKMYT1 antibody (AB200387)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PKMYT1 with ab200387 at 1/50 dilution.

Supplier Data

Western blot - Anti-PKMYT1 antibody (AB200387)

Gel : 8%SDS-PAGE

All lanes:

Western blot - Anti-PKMYT1 antibody (ab200387) at 1/200 dilution

All lanes:

Mouse heart tissue lysate at 40 µg

Predicted band size: 55 kDa

true

Exposure time: 2min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PKMYT1. The exact immunogen used to generate this antibody is proprietary information.

Q99640

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PKMYT1 also known as Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase is an important regulator of the cell cycle. It is a protein kinase with a molecular mass of about 62 kDa and is expressed in various tissues including the brain and reproductive organs. PKMYT1 has a role in regulating the entry into the mitotic phase by inhibiting the Cyclin-dependent kinase 1 (CDK1) which controls the progression through different phases of the cell cycle.

Biological function summary

This kinase is involved in controlling cell division essential for maintaining genomic integrity. PKMYT1 although not always a direct part of a physical complex significantly impacts the activation of CDK1-cyclin B complex which initiates mitosis. By phosphorylating the CDK1 complex PKMYT1 ensures proper timing of cell cycle transitions preventing premature or erroneous mitotic entry.

Pathways

PKMYT1 involvement is notable in the cell cycle regulation specifically during the G2/M transition. PKMYT1 phosphorylates CDK1 inhibiting its activity until the cell is ready to progress into mitosis. Related proteins in this pathway include CDC25 a phosphatase that removes the inhibitory phosphate groups from CDK1 allowing progression to mitosis. Through these interactions PKMYT1 tightly regulates the commitment to mitosis ensuring cells divide accurately.

Studies have linked PKMYT1 to various types of cancer due to its role in cell cycle control. Aberrations in PKMYT1 activity can lead to uncontrolled cell proliferation seen in cancers such as glioblastoma and ovarian cancer. PKMYT1 through its interactions with CDK1 and other cell cycle regulators has potential therapeutic implications. Inhibitors targeting PKMYT1 might help control tumor growth by restoring proper cell cycle regulation serving as a strategic component in cancer therapy.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins (PubMed : 10373560, PubMed : 10504341, PubMed : 9001210, PubMed : 9268380). Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation (PubMed : 9001210, PubMed : 9268380). May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect (PubMed : 9001210, PubMed : 9268380).

See full target information PKMYT1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The international journal of neuropsychopharmacology 27: PubMed39099166

2024

Identification of Phosphodiesterase-7A (PDE7A) as a Novel Target for Reducing Ethanol Consumption in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ran Wei,Fangjiao Zong,Jiahao Dong,Wei Zhao,Fangfang Zhang,Wei Wang,Shuang Zhao,Ziqi Wang,Fang Zhang,Han-Ting Zhang

Scientific reports 12:6692 PubMed35461324

2022

c-Myb-mediated inhibition of miR-601 in facilitating malignance of osteosarcoma via augmentation of PKMYT1.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Luo,Jiarui Fang,Houqing Chen,Feng He,Siying Xiao,He Liu,Shizhuang Zhu,Jianzhou Luo,Changqing Jiang

Oncology letters 23:63 PubMed35069872

2022

PKMYT1 regulates the proliferation and epithelial-mesenchymal transition of oral squamous cell carcinoma cells by targeting CCNA2.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Cai,Weidong Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PKMYT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKMYT1 antibody (AB200387)

Western blot - Anti-PKMYT1 antibody (AB200387)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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