Rabbit Recombinant Monoclonal PKMYT1 antibody. Suitable for IP, ICC/IF, WB and reacts with Human, Transfected cell lysate - Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | Flow Cyt (Intra) | ICC/IF | IHC-P | WB | |
---|---|---|---|---|---|
Human | Tested | Not recommended | Tested | Not recommended | Tested |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Transfected cell lysate - Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse, Transfected cell lysate - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rat, Mouse, Transfected cell lysate - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse, Transfected cell lysate - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rat, Mouse, Transfected cell lysate - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Human | Dilution info 1/1000 | Notes Not suitable for mouse and rat |
Species Human | Dilution info 1/1000 | Notes Not suitable for mouse and rat |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Not suitable for mouse and rat |
Species Rat | Dilution info - | Notes Not suitable for mouse and rat |
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Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.
Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase, MYT1, PKMYT1
Rabbit Recombinant Monoclonal PKMYT1 antibody. Suitable for IP, ICC/IF, WB and reacts with Human, Transfected cell lysate - Human samples.
Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase, MYT1, PKMYT1
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR27155-51
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
PKMYT1 also known as Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase is an important regulator of the cell cycle. It is a protein kinase with a molecular mass of about 62 kDa and is expressed in various tissues including the brain and reproductive organs. PKMYT1 has a role in regulating the entry into the mitotic phase by inhibiting the Cyclin-dependent kinase 1 (CDK1) which controls the progression through different phases of the cell cycle.
This kinase is involved in controlling cell division essential for maintaining genomic integrity. PKMYT1 although not always a direct part of a physical complex significantly impacts the activation of CDK1-cyclin B complex which initiates mitosis. By phosphorylating the CDK1 complex PKMYT1 ensures proper timing of cell cycle transitions preventing premature or erroneous mitotic entry.
PKMYT1 involvement is notable in the cell cycle regulation specifically during the G2/M transition. PKMYT1 phosphorylates CDK1 inhibiting its activity until the cell is ready to progress into mitosis. Related proteins in this pathway include CDC25 a phosphatase that removes the inhibitory phosphate groups from CDK1 allowing progression to mitosis. Through these interactions PKMYT1 tightly regulates the commitment to mitosis ensuring cells divide accurately.
Studies have linked PKMYT1 to various types of cancer due to its role in cell cycle control. Aberrations in PKMYT1 activity can lead to uncontrolled cell proliferation seen in cancers such as glioblastoma and ovarian cancer. PKMYT1 through its interactions with CDK1 and other cell cycle regulators has potential therapeutic implications. Inhibitors targeting PKMYT1 might help control tumor growth by restoring proper cell cycle regulation serving as a strategic component in cancer therapy.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
MYT1 is a regulator during mitosis, it may shift to different migrating forms in mitotic lysates causing different observed band sizes. PMID: 23241949, PMID: 9268380)
All lanes: Western blot - Anti-PKMYT1 antibody [EPR27155-51] (ab307146) at 1/1000 dilution
Lane 1: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate (batch 1) at 20 µg
Lane 2: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate (batch 2) at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 45-70 kDa
PKMYT1 was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug with ab307146 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307146 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug
Lane 2: ABAB307146 IP in K562 whole cell lysate
Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307146 in K562 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds
All lanes: Immunoprecipitation - Anti-PKMYT1 antibody [EPR27155-51] (ab307146) at 1/1000 dilution
Lane 1: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 μg
Lane 2: K562 whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 60 kDa, 70 kDa
Exposure time: 5s
Blocking and diluting buffer and concentration: 5% NFDM/TBSTThe molecular weight observed is consistent with what has been described in the literature (PMID: 9268380).
Nocodazole treatment results in PKMYT1 phosphorylation and a shift of MW on the gel.
Exposure time: Lanes 1-2: 81 seconds, Lane 3: 26 seconds.
All lanes: Western blot - Anti-PKMYT1 antibody [EPR27155-51] (ab307146) at 1/1000 dilution
Lane 1: Untreated K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 20 μg
Lane 2: K562 treated with /mL nocodazole for 18 hours whole cell lysate 13 μg
Lane 3: Untreated CCRF-CEM (human peripheral blood T lymphoblast) whole cell lysate 20 μg
Lane 4: CCRF-CEM treated with /mL nocodazole for 18 hours whole cell lysate 20 μg
Lane 5: K562 whole cell lysate 20 μg
Lane 6: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Observed band size: 60 kDa, 70 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 62 seconds
All lanes: Western blot - Anti-PKMYT1 antibody [EPR27155-51] (ab307146) at 1/1000 dilution
Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) transfected with scrambled siRNA control whole cell lysate 20 μg
Lane 2: K-562 transfected with siRNA specifically targeti PKMYT1 whole cell lysate 20 μg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 60 kDa, 70 kDa
Exposure time: 62s
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized K-562 (human chronic myelogenous leukemia lymphoblast) cells lABelling PKMYT1 with ab307146 at 1/50 (10.42 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in K-562 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/ml dilution.
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