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Rabbit Recombinant Monoclonal PKMYT1 antibody. Suitable for IP, ICC/IF, WB and reacts with Human, Transfected cell lysate - Human samples.

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Images

Western blot - Anti-PKMYT1 antibody [EPR27155-51] (AB307146), expandable thumbnail
  • Immunoprecipitation - Anti-PKMYT1 antibody [EPR27155-51] (AB307146), expandable thumbnail
  • Western blot - Anti-PKMYT1 antibody [EPR27155-51] (AB307146), expandable thumbnail
  • Western blot - Anti-PKMYT1 antibody [EPR27155-51] (AB307146), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PKMYT1 antibody [EPR27155-51] (AB307146), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Tested
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Not recommended
Not recommended
Not recommended
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Transfected cell lysate - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse, Transfected cell lysate - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse, Transfected cell lysate - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse, Transfected cell lysate - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell lysate - Human

Dilution info

1/1000

Notes

Not suitable for mouse and rat

Species

Human

Dilution info

1/1000

Notes

Not suitable for mouse and rat

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Not suitable for mouse and rat

Species

Rat

Dilution info

-

Notes

Not suitable for mouse and rat

Associated Products

Select an associated product type

1 products for Alternative Version

Target data

Function

Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.

Alternative names

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Rabbit Recombinant Monoclonal PKMYT1 antibody. Suitable for IP, ICC/IF, WB and reacts with Human, Transfected cell lysate - Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR27155-51

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Activity summary

PKMYT1 also known as Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase is an important regulator of the cell cycle. It is a protein kinase with a molecular mass of about 62 kDa and is expressed in various tissues including the brain and reproductive organs. PKMYT1 has a role in regulating the entry into the mitotic phase by inhibiting the Cyclin-dependent kinase 1 (CDK1) which controls the progression through different phases of the cell cycle.

Biological function summary

This kinase is involved in controlling cell division essential for maintaining genomic integrity. PKMYT1 although not always a direct part of a physical complex significantly impacts the activation of CDK1-cyclin B complex which initiates mitosis. By phosphorylating the CDK1 complex PKMYT1 ensures proper timing of cell cycle transitions preventing premature or erroneous mitotic entry.

Pathways

PKMYT1 involvement is notable in the cell cycle regulation specifically during the G2/M transition. PKMYT1 phosphorylates CDK1 inhibiting its activity until the cell is ready to progress into mitosis. Related proteins in this pathway include CDC25 a phosphatase that removes the inhibitory phosphate groups from CDK1 allowing progression to mitosis. Through these interactions PKMYT1 tightly regulates the commitment to mitosis ensuring cells divide accurately.

Associated diseases and disorders

Studies have linked PKMYT1 to various types of cancer due to its role in cell cycle control. Aberrations in PKMYT1 activity can lead to uncontrolled cell proliferation seen in cancers such as glioblastoma and ovarian cancer. PKMYT1 through its interactions with CDK1 and other cell cycle regulators has potential therapeutic implications. Inhibitors targeting PKMYT1 might help control tumor growth by restoring proper cell cycle regulation serving as a strategic component in cancer therapy.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-PKMYT1 antibody [EPR27155-51] (ab307146), expandable thumbnail

    Western blot - Anti-PKMYT1 antibody [EPR27155-51] (ab307146)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time:

    Lane 1: 60 seconds

    Lane 2: 73 seconds

    MYT1 is a regulator during mitosis, it may shift to different migrating forms in mitotic lysates causing different observed band sizes. PMID: 23241949, PMID: 9268380)

    All lanes: Western blot - Anti-PKMYT1 antibody [EPR27155-51] (ab307146) at 1/1000 dilution

    Lane 1: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate (batch 1) at 20 µg

    Lane 2: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate (batch 2) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 55 kDa

    Observed band size: 45-70 kDa

  • Immunoprecipitation - Anti-PKMYT1 antibody [EPR27155-51] (ab307146), expandable thumbnail

    Immunoprecipitation - Anti-PKMYT1 antibody [EPR27155-51] (ab307146)

    PKMYT1 was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug with ab307146 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307146 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug
    Lane 2: ABAB307146 IP in K562 whole cell lysate
    Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307146 in K562 whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5 seconds

    All lanes: Immunoprecipitation - Anti-PKMYT1 antibody [EPR27155-51] (ab307146) at 1/1000 dilution

    Lane 1: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 μg

    Lane 2: K562 whole cell lysate

    Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 60 kDa, 70 kDa

    Exposure time: 5s

  • Western blot - Anti-PKMYT1 antibody [EPR27155-51] (ab307146), expandable thumbnail

    Western blot - Anti-PKMYT1 antibody [EPR27155-51] (ab307146)

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTThe molecular weight observed is consistent with what has been described in the literature (PMID: 9268380).

    Nocodazole treatment results in PKMYT1 phosphorylation and a shift of MW on the gel.
    Exposure time: Lanes 1-2: 81 seconds, Lane 3: 26 seconds.

    All lanes: Western blot - Anti-PKMYT1 antibody [EPR27155-51] (ab307146) at 1/1000 dilution

    Lane 1: Untreated K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 20 μg

    Lane 2: K562 treated with /mL nocodazole for 18 hours whole cell lysate 13 μg

    Lane 3: Untreated CCRF-CEM (human peripheral blood T lymphoblast) whole cell lysate 20 μg

    Lane 4: CCRF-CEM treated with /mL nocodazole for 18 hours whole cell lysate 20 μg

    Lane 5: K562 whole cell lysate 20 μg

    Lane 6: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Observed band size: 60 kDa, 70 kDa

    Exposure time: 180s

  • Western blot - Anti-PKMYT1 antibody [EPR27155-51] (ab307146), expandable thumbnail

    Western blot - Anti-PKMYT1 antibody [EPR27155-51] (ab307146)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Exposure time: 62 seconds

    All lanes: Western blot - Anti-PKMYT1 antibody [EPR27155-51] (ab307146) at 1/1000 dilution

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) transfected with scrambled siRNA control whole cell lysate 20 μg

    Lane 2: K-562 transfected with siRNA specifically targeti PKMYT1 whole cell lysate 20 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 60 kDa, 70 kDa

    Exposure time: 62s

  • Immunocytochemistry/ Immunofluorescence - Anti-PKMYT1 antibody [EPR27155-51] (ab307146), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PKMYT1 antibody [EPR27155-51] (ab307146)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized K-562 (human chronic myelogenous leukemia lymphoblast) cells lABelling PKMYT1 with ab307146 at 1/50 (10.42 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in K-562 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/ml dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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