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Rabbit Recombinant Monoclonal PKMYT1 antibody. Carrier free. Suitable for IP, ICC/IF, WB and reacts with Human samples.

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Images

Western blot - Anti-PKMYT1 antibody [EPR27155-51] - BSA and Azide free (AB307147), expandable thumbnail
  • Immunoprecipitation - Anti-PKMYT1 antibody [EPR27155-51] - BSA and Azide free (AB307147), expandable thumbnail
  • Western blot - Anti-PKMYT1 antibody [EPR27155-51] - BSA and Azide free (AB307147), expandable thumbnail
  • Western blot - Anti-PKMYT1 antibody [EPR27155-51] - BSA and Azide free (AB307147), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PKMYT1 antibody [EPR27155-51] - BSA and Azide free (AB307147), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Tested
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.

    Alternative names

    Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase, MYT1, PKMYT1

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    Rabbit Recombinant Monoclonal PKMYT1 antibody. Carrier free. Suitable for IP, ICC/IF, WB and reacts with Human samples.

    Alternative names

    Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase, MYT1, PKMYT1

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    EPR27155-51

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    +4°C

    Notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Supplementary info

    Activity summary

    PKMYT1 also known as Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase is an important regulator of the cell cycle. It is a protein kinase with a molecular mass of about 62 kDa and is expressed in various tissues including the brain and reproductive organs. PKMYT1 has a role in regulating the entry into the mitotic phase by inhibiting the Cyclin-dependent kinase 1 (CDK1) which controls the progression through different phases of the cell cycle.

    Biological function summary

    This kinase is involved in controlling cell division essential for maintaining genomic integrity. PKMYT1 although not always a direct part of a physical complex significantly impacts the activation of CDK1-cyclin B complex which initiates mitosis. By phosphorylating the CDK1 complex PKMYT1 ensures proper timing of cell cycle transitions preventing premature or erroneous mitotic entry.

    Pathways

    PKMYT1 involvement is notable in the cell cycle regulation specifically during the G2/M transition. PKMYT1 phosphorylates CDK1 inhibiting its activity until the cell is ready to progress into mitosis. Related proteins in this pathway include CDC25 a phosphatase that removes the inhibitory phosphate groups from CDK1 allowing progression to mitosis. Through these interactions PKMYT1 tightly regulates the commitment to mitosis ensuring cells divide accurately.

    Associated diseases and disorders

    Studies have linked PKMYT1 to various types of cancer due to its role in cell cycle control. Aberrations in PKMYT1 activity can lead to uncontrolled cell proliferation seen in cancers such as glioblastoma and ovarian cancer. PKMYT1 through its interactions with CDK1 and other cell cycle regulators has potential therapeutic implications. Inhibitors targeting PKMYT1 might help control tumor growth by restoring proper cell cycle regulation serving as a strategic component in cancer therapy.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    5 product images

    • Western blot - Anti-PKMYT1 antibody [EPR27155-51] - BSA and Azide free (ab307147), expandable thumbnail

      Western blot - Anti-PKMYT1 antibody [EPR27155-51] - BSA and Azide free (ab307147)

      This data was developed using Anti-PKMYT1 antibody [EPR27155-51] ab307146, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Exposure time:

      Lane 1: 60 seconds

      Lane 2: 73 seconds

      MYT1 is a regulator during mitosis, it may shift to different migrating forms in mitotic lysates causing different observed band sizes. PMID: 23241949, PMID: 9268380)

      All lanes: Western blot - Anti-PKMYT1 antibody [EPR27155-51] (Anti-PKMYT1 antibody [EPR27155-51] ab307146) at 1/1000 dilution

      Lane 1: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate (batch 1) at 20 µg

      Lane 2: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate (batch 2) at 20 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

      Predicted band size: 55 kDa

      Observed band size: 45-70 kDa

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Exposure time:

      Lane 1: 60 seconds

      Lane 2: 73 seconds

      MYT1 is a regulator during mitosis, it may shift to different migrating forms in mitotic lysates causing different observed band sizes. PMID: 23241949, PMID: 9268380)

    • Immunoprecipitation - Anti-PKMYT1 antibody [EPR27155-51] - BSA and Azide free (ab307147), expandable thumbnail

      Immunoprecipitation - Anti-PKMYT1 antibody [EPR27155-51] - BSA and Azide free (ab307147)

      This data was developed using Anti-PKMYT1 antibody [EPR27155-51] ab307146, the same antibody clone in a different buffer formulation.
      PKMYT1 was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug with Anti-PKMYT1 antibody [EPR27155-51] ab307146 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-PKMYT1 antibody [EPR27155-51] ab307146 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug
      Lane 2: ABAB307146 IP in K562 whole cell lysate
      Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PKMYT1 antibody [EPR27155-51] ab307146 in K562 whole cell lysate
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 5 seconds

      All lanes: Immunoprecipitation - Anti-PKMYT1 antibody [EPR27155-51] (Anti-PKMYT1 antibody [EPR27155-51] ab307146) at 1/1000 dilution

      Lane 1: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 μg

      Lane 2: K562 whole cell lysate

      Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

      Secondary

      All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

      Observed band size: 60 kDa, 70 kDa

      Exposure time: 5s

      PKMYT1 was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug with Anti-PKMYT1 antibody [EPR27155-51] ab307146 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-PKMYT1 antibody [EPR27155-51] ab307146 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug
      Lane 2: ABAB307146 IP in K562 whole cell lysate
      Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PKMYT1 antibody [EPR27155-51] ab307146 in K562 whole cell lysate
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 5 seconds

    • Western blot - Anti-PKMYT1 antibody [EPR27155-51] - BSA and Azide free (ab307147), expandable thumbnail

      Western blot - Anti-PKMYT1 antibody [EPR27155-51] - BSA and Azide free (ab307147)

      This data was developed using 307146, the same antibody clone in a different buffer formulation.
      Blocking and diluting buffer and concentration: The molecular weight observed is consistent with what has been described in the literature (PMID: 9268380).

      Nocodazole treatment results in PKMYT1 phosphorylation and a shift of MW on the gel.
      Lanes 1-2: 81 seconds, Lane 3: 26 seconds.
      Exposure time:

      All lanes: Western blot - Anti-PKMYT1 antibody [EPR27155-51] (Anti-PKMYT1 antibody [EPR27155-51] ab307146) at 1/1000 dilution

      Lane 1: Untreated K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 20 μg

      Lane 2: K562 treated with /mL nocodazole for 18 hours whole cell lysate 13 μg

      Lane 3: Untreated CCRF-CEM (human peripheral blood T lymphoblast) whole cell lysate 20 μg

      Lane 4: CCRF-CEM treated with /mL nocodazole for 18 hours whole cell lysate 20 μg

      Lane 5: K562 whole cell lysate 20 μg

      Lane 6: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

      Observed band size: 60 kDa, 70 kDa

      Exposure time: 180s

      Blocking and diluting buffer and concentration: 5% NFDM/TBSTThe molecular weight observed is consistent with what has been described in the literature (PMID: 9268380).

      Nocodazole treatment results in PKMYT1 phosphorylation and a shift of MW on the gel.
      Exposure time: Lanes 1-2: 81 seconds, Lane 3: 26 seconds.

    • Western blot - Anti-PKMYT1 antibody [EPR27155-51] - BSA and Azide free (ab307147), expandable thumbnail

      Western blot - Anti-PKMYT1 antibody [EPR27155-51] - BSA and Azide free (ab307147)

      This data was developed using 307146, the same antibody clone in a different buffer formulation.
      Blocking and diluting buffer and concentration:
      62 seconds
      Exposure time:

      All lanes: Western blot - Anti-PKMYT1 antibody [EPR27155-51] (Anti-PKMYT1 antibody [EPR27155-51] ab307146) at 1/1000 dilution

      Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) transfected with scrambled siRNA control whole cell lysate 20 μg

      Lane 2: K-562 transfected with siRNA specifically targeti PKMYT1 whole cell lysate 20 μg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

      Observed band size: 60 kDa, 70 kDa

      Exposure time: 62s

      Blocking and diluting buffer and concentration: 5% NFDM/TBST
      Exposure time: 62 seconds

    • Immunocytochemistry/ Immunofluorescence - Anti-PKMYT1 antibody [EPR27155-51] - BSA and Azide free (ab307147), expandable thumbnail

      Immunocytochemistry/ Immunofluorescence - Anti-PKMYT1 antibody [EPR27155-51] - BSA and Azide free (ab307147)

      This data was developed using Anti-PKMYT1 antibody [EPR27155-51] ab307146, the same antibody clone in a different buffer formulation.
      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized K-562 (human chronic myelogenous leukemia lymphoblast) cells lABelling PKMYT1 with Anti-PKMYT1 antibody [EPR27155-51] ab307146 at 1/50 (10.42 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in K-562 cell line.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/ml dilution.

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