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AB195264

Anti-PKN1 antibody [EPR18808]

5

(2 Reviews)

|

(2 Publications)

Rabbit Recombinant Monoclonal PKN1 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

PAK1, PKN, PRK1, PRKCL1, PKN1, Serine/threonine-protein kinase N1, Protease-activated kinase 1, Protein kinase C-like 1, Protein kinase C-like PKN, Protein kinase PKN-alpha, Protein-kinase C-related kinase 1, Serine-threonine protein kinase N, PAK-1

5 Images
Immunoprecipitation - Anti-PKN1 antibody [EPR18808] (AB195264)
  • IP

Supplier Data

Immunoprecipitation - Anti-PKN1 antibody [EPR18808] (AB195264)

PKN1 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with ab195264 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab195264 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Jurkat whole cell lysate, 10μg (Input).

Lane 2 : ab195264 IP in Jurkat whole cell lysate.

Lane 3 : Rabbit IgG,monoclonal-Isotype Control (ab172730) instead of ab195264 in Jurkat whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-PKN1 antibody [EPR18808] (ab195264)

Predicted band size: 104 kDa

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Western blot - Anti-PKN1 antibody [EPR18808] (AB195264)
  • WB

Lab

Western blot - Anti-PKN1 antibody [EPR18808] (AB195264)

Lane 1 : Wild-type HAP1 cell lysate (40 μg)
Lane 2 : PKN1 knockout HAP1 cell lysate (40 μg)
Lane 3 : MCF7 cell lysate (20 μg)
Lane 4 : K562 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab195264 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab195264 was shown to specifically react with PKN1 when PKN1 knockout samples were used. Wild-type and PKN1 knockout samples were subjected to SDS-PAGE. ab195264 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PKN1 antibody [EPR18808] (ab195264)

Predicted band size: 104 kDa

false

Western blot - Anti-PKN1 antibody [EPR18808] (AB195264)
  • WB

Supplier Data

Western blot - Anti-PKN1 antibody [EPR18808] (AB195264)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKN1 antibody [EPR18808] (ab195264) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 2:

LNCaP (Human prostate cancer cell line) whole cell lysate at 20 µg

Lane 3:

MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 4:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

Lane 5:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 6:

MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 104 kDa

Observed band size: 120 kDa

false

Exposure time: 3min

Western blot - Anti-PKN1 antibody [EPR18808] (AB195264)
  • WB

Supplier Data

Western blot - Anti-PKN1 antibody [EPR18808] (AB195264)

All lanes:

Western blot - Anti-PKN1 antibody [EPR18808] (ab195264) at 1/1000 dilution

Lane 1:

Rat brain lysate at 10 µg

Lane 2:

Rat spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 104 kDa

Observed band size: 120 kDa

false

Exposure time: 3min

Western blot - Anti-PKN1 antibody [EPR18808] (AB195264)
  • WB

Supplier Data

Western blot - Anti-PKN1 antibody [EPR18808] (AB195264)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKN1 antibody [EPR18808] (ab195264) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 104 kDa

Observed band size: 120 kDa

false

Exposure time: 3min

  • Carrier free

    Anti-PKN1 antibody [EPR18808] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18808

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.
See full target information PKN1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 10:3902 PubMed32127582

2020

Novel roles of PRK1 and PRK2 in cilia and cancer biology.

Applications

Unspecified application

Species

Unspecified reactive species

Hitesh Patel,Jun Li,Ana Herrero,Jakob Kroboth,Adam Byron,Alex Von Kriegsheim,Valerie Brunton,Neil Carragher,Toby Hurd,Margaret Frame

Nature communications 9:3688 PubMed30206226

2018

Exome-wide analysis identifies three low-frequency missense variants associated with pancreatic cancer risk in Chinese populations.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang Chang,Jianbo Tian,Ying Zhu,Rong Zhong,Kan Zhai,Jiaoyuan Li,Juntao Ke,QiangQiang Han,Jiao Lou,Wei Chen,Beibei Zhu,Na Shen,Yi Zhang,Yajie Gong,Yang Yang,Danyi Zou,Xiating Peng,Zhi Zhang,Xuemei Zhang,Kun Huang,Ming Yang,Li Wang,Chen Wu,Dongxin Lin,Xiaoping Miao
View all publications
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Product promise

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