AB138514

Anti-PKN2 antibody [EPR5490]

RabMAb
Recombinant
KO Validated
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(4 Publications)

Rabbit Recombinant Monoclonal PKN2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

PRK2, PRKCL2, PKN2, Serine/threonine-protein kinase N2, PKN gamma, Protein kinase C-like 2, Protein-kinase C-related kinase 2

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-PKN2 antibody [EPR5490] (AB138514)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PKN2 antibody [EPR5490] (AB138514)

Immunofluorescent analysis of HeLa cells labelling PKN2 with ab138514 at 1/100 dilution.

Unknown

Western blot - Anti-PKN2 antibody [EPR5490] (AB138514)

Lanes 1-3 : Merged signal (red and green). Green - ab138514 observed at 112 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab138514 Anti-PKN2 antibody [EPR5490] was shown to specifically react with PKN2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264691 (knockout cell lysate ab258587) was used. Wild-type and PKN2 knockout samples were subjected to SDS-PAGE. ab138514 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PKN2 antibody [EPR5490] (ab138514) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PKN2 knockout HeLa cell lysate at 20 µg

Lane 3:

293T cell lysate at 20 µg

Predicted band size: 112 kDa

Observed band size: 112 kDa

false

Lab

Western blot - Anti-PKN2 antibody [EPR5490] (AB138514)

Lanes 1-3 : Merged signal (red and green). Green - ab138514 observed at 112 kDa. Red - loading control, ab8245 observed at 37 kDa.

All lanes:

Western blot - Anti-PKN2 antibody [EPR5490] (ab138514) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PKN2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PKN2 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-pkn2-knockout-hela-cell-line-ab264691'>ab264691</a>)

Lane 3:

HEK-293T cell lysate at 20 µg

Predicted band size: 112 kDa

Observed band size: 112 kDa

false

Lab

Western blot - Anti-PKN2 antibody [EPR5490] (AB138514)

Lanes 1-3 : Merged signal (red and green). Green - ab138514 observed at 125 kDa. Red - loading control ab8245 observed at 36 kDa.

ab138514 Anti-PKN2 antibody [EPR5490] (ab138514) was shown to specifically react with PKN2 in wild-type HCT cells. Loss of signal was observed when knockout cell line ab266894 (knockout cell lysate ab258588) was used. Wild-type and PKN2 knockout samples were subjected to SDS-PAGE. ab138514 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PKN2 antibody [EPR5490] (ab138514) at 1/1000 dilution

Lane 1:

Wild-type HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

PKN2 knockout HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human PKN2 knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-pkn2-knockout-hct116-cell-line-ab266894'>ab266894</a>)

Lane 3:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 112 kDa

Observed band size: 125 kDa

false

Unknown

Western blot - Anti-PKN2 antibody [EPR5490] (AB138514)

All lanes:

Western blot - Anti-PKN2 antibody [EPR5490] (ab138514) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 112 kDa

Observed band size: 112 kDa

false

Carrier free

Anti-PKN2 antibody [EPR5490] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5490

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Involved in the negative regulation of ciliogenesis (PubMed : 27104747).. (Microbial infection) Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication.

See full target information PKN2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 26:5713-5727 PubMed36308410

2022

Phosphorylation-mediated PI3K-Art signalling pathway as a therapeutic mechanism in the hydrogen-induced alleviation of brain injury in septic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Bai,Li Li,Beibei Dong,Wanjie Ma,Hongguang Chen,Yonghao Yu

International journal of endocrinology 2021:5499963 PubMed34745257

2021

Overexpression of PAX8-AS1 Inhibits Malignant Phenotypes of Papillary Thyroid Carcinoma Cells via miR-96-5p/PKN2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Zhou,Tongdao Xu,Hao Hu,Fei Hua

Cancer biology & therapy 22:311-323 PubMed33879018

2021

LINC00668 cooperated with HuR dependent upregulation of PKN2 to facilitate gastric cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jutang Li,Wei Dong,Qixia Jiang,Fenglian Zhang,Hui Dong

Cell cycle (Georgetown, Tex.) 15:678-88 PubMed27104747

2016

STAR syndrome-associated CDK10/Cyclin M regulates actin network architecture and ciliogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Vincent J Guen,Carly Gamble,Dahlia E Perez,Sylvie Bourassa,Hildegard Zappel,Jutta Gärtner,Jacqueline A Lees,Pierre Colas

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PKN2 antibody [EPR5490]

Immunocytochemistry/ Immunofluorescence - Anti-PKN2 antibody [EPR5490] (AB138514)

Western blot - Anti-PKN2 antibody [EPR5490] (AB138514)

Western blot - Anti-PKN2 antibody [EPR5490] (AB138514)

Western blot - Anti-PKN2 antibody [EPR5490] (AB138514)

Western blot - Anti-PKN2 antibody [EPR5490] (AB138514)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise