AB124709

Anti-PKN3 (phospho T718) + PRK1 (phospho T774) + PKN2 (phospho T816) antibody [EPR5671]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal PKN3 phospho T718 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

PKNBETA, PKN3, Serine/threonine-protein kinase N3, Protein kinase PKN-beta, Protein-kinase C-related kinase 3, PAK1, PKN, PRK1, PRKCL1, PKN1, Serine/threonine-protein kinase N1, Protease-activated kinase 1, Protein kinase C-like 1, Protein kinase C-like PKN, Protein kinase PKN-alpha, Protein-kinase C-related kinase 1, Serine-threonine protein kinase N, PAK-1, PRK2, PRKCL2, PKN2, Serine/threonine-protein kinase N2, PKN gamma, Protein kinase C-like 2, Protein-kinase C-related kinase 2

2 Images

Flow Cytometry (Intracellular) - Anti-PKN3 (phospho T718) + PRK1 (phospho T774) + PKN2 (phospho T816) antibody [EPR5671] (AB124709)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PKN3 (phospho T718) + PRK1 (phospho T774) + PKN2 (phospho T816) antibody [EPR5671] (AB124709)

ab124709, at 1/10, staining PKN1 (phospho T774) + PKN2 (phospho T816) + PKN3 (phospho T718) in permeabilized Hek 293 cells by intracellular flow cytometry. Green : untreated cells Red : Okadaic Acid + Calyculin A-treated cells Blue : untreated cells preincubated with the immunizing phosphopeptides Orange : Okadaic Acid + Calyculin A-treated cells preincubated with the immunizing phosphopeptides.

Unknown

Western blot - Anti-PKN3 (phospho T718) + PRK1 (phospho T774) + PKN2 (phospho T816) antibody [EPR5671] (AB124709)

Bottom blot was probed with an anti-PNK2 antibody.

All lanes:

Western blot - Anti-PKN3 (phospho T718) + PRK1 (phospho T774) + PKN2 (phospho T816) antibody [EPR5671] (ab124709) at 1/1000 dilution

Lane 1:

HeLa cell lysate untreated at 10 µg

Lane 2:

HeLa cell lysate treated with Okadaic acid and Calyculin A. at 10 µg

Predicted band size: 104 kDa,112 kDa

false

Carrier free

Anti-PKN3 (pT718) + PRK1 (pT774) + PKN2 (pT816) antibody [EPR5671] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5671

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

This product was produced with the following immunogens:

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects PKN3 phosphorylated at Threonine 718, PKN2 phosphorylated at Threonine 816, and PKN1 phosphorylated at Threonine 774.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Contributes to invasiveness in malignant prostate cancer.

See full target information PKN3 pT718

Additional targets

PKN1 phospho T774,PKN2 phospho T816

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 26:5713-5727 PubMed36308410

2022

Phosphorylation-mediated PI3K-Art signalling pathway as a therapeutic mechanism in the hydrogen-induced alleviation of brain injury in septic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Bai,Li Li,Beibei Dong,Wanjie Ma,Hongguang Chen,Yonghao Yu

Science signaling 10: PubMed28851822

2017

Protein kinase N3 promotes bone resorption by osteoclasts in response to Wnt5a-Ror2 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shunsuke Uehara,Nobuyuki Udagawa,Hideyuki Mukai,Akihiro Ishihara,Kazuhiro Maeda,Teruhito Yamashita,Kohei Murakami,Michiru Nishita,Takashi Nakamura,Shigeaki Kato,Yasuhiro Minami,Naoyuki Takahashi,Yasuhiro Kobayashi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PKN3 (phospho T718) + PRK1 (phospho T774) + PKN2 (phospho T816) antibody [EPR5671]

Flow Cytometry (Intracellular) - Anti-PKN3 (phospho T718) + PRK1 (phospho T774) + PKN2 (phospho T816) antibody [EPR5671] (AB124709)

Western blot - Anti-PKN3 (phospho T718) + PRK1 (phospho T774) + PKN2 (phospho T816) antibody [EPR5671] (AB124709)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Additional targets

Publications (2)

Product promise