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AB247973

Anti-PKN3 (pT718) + PRK1 (pT774) + PKN2 (pT816) antibody [EPR5671] - BSA and Azide free

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Rabbit Recombinant Monoclonal PKN3 phospho T718 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

PKNBETA, PKN3, Serine/threonine-protein kinase N3, Protein kinase PKN-beta, Protein-kinase C-related kinase 3

2 Images
Flow Cytometry (Intracellular) - Anti-PKN3 (pT718) + PRK1 (pT774) + PKN2 (pT816) antibody [EPR5671] - BSA and Azide free (AB247973)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PKN3 (pT718) + PRK1 (pT774) + PKN2 (pT816) antibody [EPR5671] - BSA and Azide free (AB247973)

This data was developed using ab124709, the same antibody clone in a different buffer formulation.

ab124709, at 1/10, staining PKN1 (phospho T774) + PKN2 (phospho T816) + PKN3 (phospho T718) in permeabilized Hek 293 cells by intracellular flow cytometry. Green : untreated cells Red : Okadaic Acid + Calyculin A-treated cells Blue : untreated cells preincubated with the immunizing phosphopeptides Orange : Okadaic Acid + Calyculin A-treated cells preincubated with the immunizing phosphopeptides .

Western blot - Anti-PKN3 (pT718) + PRK1 (pT774) + PKN2 (pT816) antibody [EPR5671] - BSA and Azide free (AB247973)
  • WB

Unknown

Western blot - Anti-PKN3 (pT718) + PRK1 (pT774) + PKN2 (pT816) antibody [EPR5671] - BSA and Azide free (AB247973)

This data was developed using ab124709, the same antibody clone in a different buffer formulation.

Bottom blot was probed with an anti-PNK2 antibody.

All lanes:

Western blot - Anti-PKN3 (phospho T718) + PRK1 (phospho T774) + PKN2 (phospho T816) antibody [EPR5671] (<a href='/en-us/products/primary-antibodies/pkn3-phospho-t718-prk1-phospho-t774-pkn2-phospho-t816-antibody-epr5671-ab124709'>ab124709</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate untreated at 10 µg

Lane 2:

HeLa cell lysate treated with Okadaic acid and Calyculin A. at 10 µg

Predicted band size: 104 kDa,112 kDa

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  • Unconjugated

    Anti-PKN3 (phospho T718) + PRK1 (phospho T774) + PKN2 (phospho T816) antibody [EPR5671]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5671

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects PKN3 phosphorylated at Threonine 718, PKN2 phosphorylated at Threonine 816, and PKN1 phosphorylated at Threonine 774.

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Reactivity data

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Product details

ab247973 is the carrier-free version of ab124709.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Contributes to invasiveness in malignant prostate cancer.
See full target information PKN3 phospho T718

Additional targets

PKN2 phospho T816,PKN1 phospho T774
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com