Skip to main content

Phospho PKR antibody pT446 [E120] ab32036 is a rabbit monoclonal antibody that is used in PKR western blotting. Suitable for human samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone E120 is the most widely used clone for PKR on the market and is cited in >130 publications


Images

Immunoprecipitation - Anti-PKR (phospho T446) antibody [E120] (AB32036), expandable thumbnail
  • Western blot - Anti-PKR (phospho T446) antibody [E120] (AB32036), expandable thumbnail
  • Western blot - Anti-PKR (phospho T446) antibody [E120] (AB32036), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Not recommended
Tested
Not recommended
Tested
Not recommended
Pig
Not recommended
Predicted
Not recommended
Predicted
Not recommended

Not recommended
Not recommended

Species

Pig, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10

Notes

-

Predicted
Predicted

Species

Pig

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Pig

Dilution info

-

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Human

Dilution info

-

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Predicted
Predicted

Species

Pig

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Pig, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

IFN-induced dsRNA-dependent serine/threonine-protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) and plays a key role in the innate immune response to viral infection (PubMed:18835251, PubMed:19189853, PubMed:19507191, PubMed:21072047, PubMed:21123651, PubMed:22381929, PubMed:22948139, PubMed:23229543). Inhibits viral replication via the integrated stress response (ISR): EIF2S1/eIF-2-alpha phosphorylation in response to viral infection converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, resulting to a shutdown of cellular and viral protein synthesis, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4 (PubMed:19189853, PubMed:21123651, PubMed:22948139, PubMed:23229543). Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1) (PubMed:11836380, PubMed:19189853, PubMed:19840259, PubMed:20171114, PubMed:21710204, PubMed:23115276, PubMed:23399035). Also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation: phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11 (PubMed:11836380, PubMed:19229320, PubMed:22214662). In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteasomal degradation (PubMed:20395957). Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding pro-inflammatory cytokines and IFNs (PubMed:22948139, PubMed:23084476, PubMed:23372823). Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6 (PubMed:10848580, PubMed:15121867, PubMed:15229216). Can act as both a positive and negative regulator of the insulin signaling pathway (ISP) (PubMed:20685959). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2) (PubMed:20685959). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes (PubMed:22801494). Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin (By similarity).

Alternative names

Recommended products

Phospho PKR antibody pT446 [E120] ab32036 is a rabbit monoclonal antibody that is used in PKR western blotting. Suitable for human samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone E120 is the most widely used clone for PKR on the market and is cited in >130 publications

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

E120

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunoprecipitation - Anti-PKR (phospho T446) antibody [E120] (ab32036), expandable thumbnail

    Immunoprecipitation - Anti-PKR (phospho T446) antibody [E120] (ab32036)

    PKR was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) treated with Calyculin A(100nM 30min) whole cell lysate 10 μg with ab32036 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) treated with Calyculin A(100nM 30min) whole cell lysate 10 μg

    Lane 2: ab32036 IP in HeLa treated with Calyculin A(100nM 30min) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab32036 in HeLa treated with Calyculin A(100nM 30min) whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Lower bands could be cleavage form. (PMID:28702377)

    All lanes: Immunoprecipitation - Anti-PKR (phospho T446) antibody [E120] (ab32036)

    Predicted band size: 62 kDa

    Observed band size: 68 kDa

  • Western blot - Anti-PKR (phospho T446) antibody [E120] (ab32036), expandable thumbnail

    Western blot - Anti-PKR (phospho T446) antibody [E120] (ab32036)

    Blocking/Diluting buffer: 5% NFDM/TBST

    Positive control: Rabbit monoclonal [Y117] to PKR (Anti-PKR antibody [Y117] ab32506).

    All lanes: Western blot - Anti-PKR (phospho T446) antibody [E120] (ab32036) at 1/2000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma) whole cell lysate at 15 µg

    Lane 2: HeLa (human cervix adenocarcinoma) treated with 100 ng/mL Calyculin A whole cell lysate at 15 µg

    Lane 3: HeLa (human cervix adenocarcinoma) treated with 100 ng/mL Calyculin A followed by 20 ng/mL TNF alpha whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 62 kDa

    Observed band size: 68 kDa

    Exposure time: 10s

    Blocking/Diluting buffer: 5% NFDM/TBST

    Positive control: Rabbit monoclonal [Y117] to PKR (Anti-PKR antibody [Y117] ab32506).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32036).

  • Western blot - Anti-PKR (phospho T446) antibody [E120] (ab32036), expandable thumbnail

    Western blot - Anti-PKR (phospho T446) antibody [E120] (ab32036)

    All lanes: Western blot - Anti-PKR (phospho T446) antibody [E120] (ab32036) at 1/1000 dilution

    Lane 1: Hela (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Lane 2: Hela cell lysate + IFN + CalyculinA

    Lane 3: Hela cell lysate + Pptase

    Predicted band size: 62 kDa

    Observed band size: 68 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com