Mouse Monoclonal PLA2R antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLA2R1 aa 1-700.
Images
![Western blot - Anti-PLA2R antibody [12-6-5] (AB211490), expandable thumbnail](https://content.abcam.com/products/images/pla2r-antibody-12-6-5-ab211490--western-blot-img61441.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PLA2R antibody [12-6-5] (AB211490)")
![Flow Cytometry - Anti-PLA2R antibody [12-6-5] (AB211490), expandable thumbnail](https://content.abcam.com/products/images/pla2r-antibody-12-6-5-ab211490--flow-cytometry-img39803.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-PLA2R antibody [12-6-5] (AB211490)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (AB211490), expandable thumbnail](https://content.abcam.com/products/images/pla2r-antibody-12-6-5-ab211490--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111185.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (AB211490)")
![Immunocytochemistry/ Immunofluorescence - Anti-PLA2R antibody [12-6-5] (AB211490), expandable thumbnail](https://content.abcam.com/products/images/pla2r-antibody-12-6-5-ab211490--immunocytochemistry-immunofluorescence-img89746.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PLA2R antibody [12-6-5] (AB211490)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (AB211490), expandable thumbnail](https://content.abcam.com/products/images/pla2r-antibody-12-6-5-ab211490--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102131.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (AB211490)")
Publications
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- Nephrology, dialysis, transplantation : official p :2020Seropositive PLA2R-associated membranous nephropathy but biopsy-negative PLA2R staining.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiao Luo et. al.PubMed 33236093
- American journal of physiology. Cell physiology 315:C398-C4082018Human soluble phospholipase A receptor is an inhibitor of the integrin-mediated cell migratory response to collagen-I.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazunori Watanabe et. al.PubMed 29791208
- Scientific reports 7:68762017PLAR binds to the annexin A2-S100A10 complex in human podocytes.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human PLA2R1 aa 1-700. The exact immunogen used to generate this antibody is proprietary information. Database link Q13018
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000.00000 - 1/10000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000.00000 - 1/20000.00000 | Notes Perform heat mediated antigen retrieval at pH 8 before commencing with IHC staining protocol. See attached document for protocol details. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/400 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phospholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in pro-inflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B (PubMed:15611272, PubMed:7721806). In podocytes, binding of sPLA2-IB/PLA2G1B can regulate podocyte survival and glomerular homeostasis (PubMed:25335547).
Alternative names
CLEC13C, PLA2R1, Secretory phospholipase A2 receptor, PLA2-R, PLA2R, 180 kDa secretory phospholipase A2 receptor, C-type lectin domain family 13 member C, M-type receptor
Recommended products
Mouse Monoclonal PLA2R antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PLA2R1 aa 1-700.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human PLA2R1 aa 1-700. The exact immunogen used to generate this antibody is proprietary information. Database link Q13018
- Clone number
- 12-6-5
- Purification technique
- Affinity purification Protein G
- Dissociation constant
- 5.4 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-PLA2R antibody [12-6-5] (ab211490), expandable thumbnail](https://content.abcam.com/products/images/pla2r-antibody-12-6-5-ab211490--western-blot-img61441.jpg "Western blot - Anti-PLA2R antibody [12-6-5] (ab211490)")
Western blot - Anti-PLA2R antibody [12-6-5] (ab211490)
All lanes: Western blot - Anti-PLA2R antibody [12-6-5] (ab211490) at 1/10000 dilution
Lane 1: Recombinant extracellular domain of human PLA2R (NC8) at 10 µg
Lane 2: Recombinant N-terminus fragment of human PLA2R (NC3) at 10 µg
Predicted band size: 169 kDa
![Flow Cytometry - Anti-PLA2R antibody [12-6-5] (ab211490), expandable thumbnail](https://content.abcam.com/products/images/pla2r-antibody-12-6-5-ab211490--flow-cytometry-img39803.jpg "Flow Cytometry - Anti-PLA2R antibody [12-6-5] (ab211490)")
Flow Cytometry - Anti-PLA2R antibody [12-6-5] (ab211490)
Flow cytometry analysis of wild type (dark blue line) and over-expressing PLA2R podocytes (low expressor, green line; high expressor, red line) labeling PLA2R using ab211490 at 1/200 dilution. Negative controls were unlabelled cells (turquoise line) and mouse IgG (grey line).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (ab211490), expandable thumbnail](https://content.abcam.com/products/images/pla2r-antibody-12-6-5-ab211490--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111185.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (ab211490)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (ab211490)
Immunohistochemical analysis of membranous nephropathy kidney tissue labeling PLA2R at 1/5000 dilution followed by DAB detection.
![Immunocytochemistry/ Immunofluorescence - Anti-PLA2R antibody [12-6-5] (ab211490), expandable thumbnail](https://content.abcam.com/products/images/pla2r-antibody-12-6-5-ab211490--immunocytochemistry-immunofluorescence-img89746.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PLA2R antibody [12-6-5] (ab211490)")
Immunocytochemistry/ Immunofluorescence - Anti-PLA2R antibody [12-6-5] (ab211490)
Immunofluorescent analysis of PLA2R over-expressing podocytes labeling PLA2R at 1/400 dilution (green).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (ab211490), expandable thumbnail](https://content.abcam.com/products/images/pla2r-antibody-12-6-5-ab211490--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102131.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (ab211490)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (ab211490)
Immunohistochemical analysis of normal human kidney tissue labeling PLA2R at 1/5000 dilution followed by DAB detection.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (ab211490), expandable thumbnail](https://content.abcam.com/products/images/pla2r-antibody-12-6-5-ab211490--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img97764.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (ab211490)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (ab211490)
Immunohistochemical analysis of normal human kidney tissue labeling PLA2R at 1/5000 dilution followed by DAB detection.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (ab211490), expandable thumbnail](https://content.abcam.com/products/images/pla2r-antibody-12-6-5-ab211490--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img97764.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [12-6-5] (ab211490)")
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