Goat Polyclonal PLA2R antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Secretory phospholipase A2 receptor aa 900-1000.
Images
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human Secretory phospholipase A2 receptor aa 900-1000. The exact immunogen used to generate this antibody is proprietary information. Database link Q13018
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Cow | Predicted |
| Orangutan | Predicted |
| Rabbit | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-3.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan, Rabbit | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phospholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in pro-inflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B (PubMed:15611272, PubMed:7721806). In podocytes, binding of sPLA2-IB/PLA2G1B can regulate podocyte survival and glomerular homeostasis (PubMed:25335547).
Alternative names
CLEC13C, PLA2R1, CLEC13C, Secretory phospholipase A2 receptor, PLA2-R, PLA2R, 180 kDa secretory phospholipase A2 receptor, C-type lectin domain family 13 member C, M-type receptor
Recommended products
Goat Polyclonal PLA2R antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Secretory phospholipase A2 receptor aa 900-1000.
Key facts
- Isotype
IgG
- Host species
Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human Secretory phospholipase A2 receptor aa 900-1000. The exact immunogen used to generate this antibody is proprietary information. Database link Q13018
- Purification technique
Affinity purification Immunogen
- Specificity
ab99466 is expected to recognize both isoforms (NP_031392.3; NP_001007268.1).
- Concentration
- Purification notes
ab99466 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-PLA2R antibody (ab99466)
Western Blot analysis showing approximate 150kDa band observed in Human Kidney and Pig Lung lysates.
All lanes: Western blot - Anti-PLA2R antibody (ab99466)
Predicted band size: 169 kDa
Observed band size: 150 kDa
Western blot - Anti-PLA2R antibody (ab99466)
All lanes: Western blot - Anti-PLA2R antibody (ab99466) at 1 µg/mL
All lanes: Human Ileum lysate at 35 µg
Predicted band size: 169 kDa
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