AB188028

Anti-PLA2R antibody [CL0474]

Name: Anti-PLA2R antibody [CL0474] (ab188028) | Abcam
Brand: Abcam Limited
SKU: AB188028
Price: 470 USD
Availability: InStock

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(4 Publications)

Anti-PLA2R antibody [CL0474] (ab188028) is a mouse monoclonal antibody detecting PLA2R in Western Blot, IHC-P. Suitable for Human.

View Alternative Names

CLEC13C, PLA2R1, Secretory phospholipase A2 receptor, PLA2-R, PLA2R, 180 kDa secretory phospholipase A2 receptor, C-type lectin domain family 13 member C, M-type receptor

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [CL0474] (AB188028)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [CL0474] (AB188028)

Immunohistochemical staining (using ab188028) of human kidney with membranous nephropathy shows strong membranous immunoreactivity in renal glomeruli.

Supplier Data

Immunocytochemistry - Anti-PLA2R antibody [CL0474] (AB188028)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PLA2R with ab188028 at 10 μg/mL, showing specific staining in the cytosol and plasma membrane in green. Nuclear probes are visualized in blue.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [CL0474] (AB188028)

Immunohistochemical staining (using ab188028) of human kidney shows only low background positivity.

Supplier Data

Western blot - Anti-PLA2R antibody [CL0474] (AB188028)

All lanes:

Western blot - Anti-PLA2R antibody [CL0474] (ab188028) at 1 µg/mL

All lanes:

Human kidney tissue lysate

Predicted band size: 169 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0474

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC

applications

Immunogen

Recombinant Fragment Protein within Human PLA2R1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13018

Epitope

Binds to an epitope located within the peptide sequence KIPVSFEWSN as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20000 - 1/50000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2-10 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-PLA2R antibody [CL0474] (ab188028) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of PLA2R?
Anti-PLA2R [CL0474] (ab188028) specifically detects a band for PLA2R (UniProt: Q13018) at a molecular weight of 169kDa.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phospholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in pro-inflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B (PubMed : 15611272, PubMed : 7721806). In podocytes, binding of sPLA2-IB/PLA2G1B can regulate podocyte survival and glomerular homeostasis (PubMed : 25335547).

See full target information PLA2R1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Renal failure 44:304-313 PubMed35333675

2022

Anti-phospholipase A2 receptor antibodies directly induced podocyte damage .

Applications

Unspecified application

Species

Unspecified reactive species

Yanfen Li,Juntao Yu,Miao Wang,Zhao Cui,Ming-Hui Zhao

Kidney international reports 6:1992-1995 PubMed34307995

2021

NELL1-Associated Membranous Glomerulopathy After Hematopoietic Stem Cell Transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Satoru Kudose,Miroslav Sekulic,Collette J Mehring,Dominick Santoriello,Ibrahim Batal,M Barry Stokes,Vivette D D'Agati,Glen S Markowitz

American journal of physiology. Cell physiology 315:C398-C408 PubMed29791208

2018

Human soluble phospholipase A receptor is an inhibitor of the integrin-mediated cell migratory response to collagen-I.

Applications

Unspecified application

Species

Unspecified reactive species

Kazunori Watanabe,Kazuhiro Watanabe,Yosuke Watanabe,Daisuke Fujioka,Takamitsu Nakamura,Kazuto Nakamura,Jun-Ei Obata,Kiyotaka Kugiyama

Molecular medicine reports 16:9347-9354 PubMed29039590

2017

MicroRNA‑218 promotes prostaglandin E2 to inhibit osteogenic differentiation in synovial mesenchymal stem cells by targeting 15‑hydroxyprostaglandin dehydrogenase [NAD(+)].

Applications

Unspecified application

Species

Unspecified reactive species

Ruijun Cong,Kun Tao,Peiliang Fu,Lieming Lou,Yuchang Zhu,Song Chen,Xinyu Cai,Lingzhou Mao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PLA2R antibody [CL0474]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [CL0474] (AB188028)

Immunocytochemistry - Anti-PLA2R antibody [CL0474] (AB188028)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [CL0474] (AB188028)

Western blot - Anti-PLA2R antibody [CL0474] (AB188028)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise